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  • 1
    Electronic Resource
    Electronic Resource
    I. Photoaffinity probes provide a general method to prepare synthetic peptide-conjugates (1984)
    Springer
    The protein journal 3 (1984), S. 465-478 
    Details
    ISSN: 1573-4943
    Keywords: photoaffinity probes ; synthetic peptide-conjugates ; solid-phase peptide synthesis ; benzophenone ; antibodies to synthetic peptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A general synthetic strategy is described for the preparation of peptide-conjugates where the peptides contain the NH2 terminal, COOH terminal, or internal regions of the protein sequence. Glycoprotein D of herpes simplex virus type 1 is used as a representative protein. Ten-residue peptide fragments of the native sequence were synthesized using standard solid-phase methodology. Photoprobes stable to conditions of synthesis and HF cleavage were coupled directly to the protected-peptide resin during synthesis. This one-step procedure eliminates the potential modification of functional groups in the sequence of interest that can occur when using chemically labile bifunctional reagents. Since the photoprobe is inert until photolysis, the synthetic peptide-probe can be readily purified by high-performance liquid chromatography before cross-linking to the carrier molecule. The following photoprobe derivatives were investigated: thep-azidobenzoyl,p-nitrophenylalanyl, andp-benzoylbenzoyl groups. The benzophenone photoprobes were shown to give the highest incorporation of peptide-probe with the protein carrier over a wide range ofpH and solvent conditions. For solid-phase synthesis three benzophenone photoprobes can be used: benzoylbenzoic acid, benzoylbenzoylglycine, andN e-(4-benzoylbenzoyl)-N α-t-butyloxycarbonyl-lysine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01025065
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  • 2
    Electronic Resource
    Electronic Resource
    II. Photoaffinity probes provide a general method to prepare peptide-conjugates from native protein fragments (1984)
    Springer
    The protein journal 3 (1984), S. 479-489 
    Details
    ISSN: 1573-4943
    Keywords: photoaffinity probes ; peptide-conjugates from native protein fragments ; benzophenone probes ; p-azidobenzoyl probes ; antibodies to protein fragments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The advantages of using amino-directed photoprobes to couple native fragments, obtained by enzymatic digestion with trypsin, to protein carriers to prepare peptide-conjugates is described. The following photoprobe reagents were investigated:N-hydroxysuccinimidylp-azidobenzoate,N-hydroxysuccinimidyl ester ofp-azidobenzoylglycine,N-hydroxysuccinimidylp-benzoylbenzoate, and pentachlorophenyl ester ofp-benzoylbenzoyl glycine or the symmetric anhydride ofp-benzoylbenzoylglycine. These reagents modify only the NH2-terminal amino group and/or COOH-terminal ε-amino group of lysine of the tryptic fragments. Since the photoprobe is inert until photolysis, the probe-modified native fragment can be readily purified by high-performance liquid chromatography before cross-linking to the carrier molecule. The benzophenone photoprobes were shown to give the highest incorporation of peptide onto the protein carrier.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01025066
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  • 3
    Electronic Resource
    Electronic Resource
    Phonoangiography: Qualitative and quantitative (1984)
    Springer
    Annals of biomedical engineering 12 (1984), S. 55-62 
    Details
    ISSN: 1573-9686
    Keywords: Carotid stenosis ; Bruit analysis ; Sound spectral analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Bruit analysis (phonoangiography) has been performed for many years as a method of characterizing arterial disease. Time displays of arterial bruits, particularly at the carotid bifurcation, have been used in an attempt to quantitate arterial narrowing. Despite the generalization that longer bruits and bruits which look and sound higher in frequency are often associated with severe disease, prospective studies have shown no useful predictive value for qualitative phonoangiography. In marked contrast, spectral bruit analysis or quantitative phonoangiography has been quite accurate in predicting the location and extent of carotid stenosis, and in distinguishing intrinsic from transmitted bruits. With this method, the peak systolic portion of the bruit is subjected to fast Fourier transform analysis. The peak frequency, beyond which amplitude drops as frequency increases further, is directly related to the residual lumen diameter of the stenotic common or internal carotid artery. Several blinded trials of this method have given results accurate to within 1 mm of angiographic values in 83–93% of cases studied. When used in conjunction with duplex doppler ultrasound scanning, 95% accuracy in diagnosis of patients with and without bruits may be achieved. This completely noninvasive method deserves more widespread use and may also be applicable to other cardiovascular sounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02410291
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  • 4
    Electronic Resource
    Electronic Resource
    State of the art in fermentation air filtration (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 844-847 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article presents several characteristics of a state-of-the-art fermentation air filter. The filter medium is composed solely of PTFE and has an absolute pore size rating of 0.2 μm. Quantitative bacteria and bacteriophage retention is shown based on live organism challenge tests. A nondestructive filter test, correlated to the microorganism challenge tests and called the Forward Flow Integrity Test, is described. This test has a sensitivity of one part in 1012 and can be performed in situ.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260805
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  • 5
    Electronic Resource
    Electronic Resource
    Kinetics of growth of the hydrogen-oxidizing bacterium Alcaligenes eutrophus (ATCC 17707) in chemostat culture (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 764-770 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The hydrogen-oxidizing bacterium, Alcaligenes eutrophus (ATCC 17707), was grown in chemostat culture with gas-phase (hydrogen, oxygen, and carbon dioxide) and liquid-phase (mineral nutrients) feedstreams; data were used to generate an analytical form for the specific growth rate equation. Model parameters obtained include Monod rate parameters for dissolved hydrogen and oxygen gases, yield coefficients, and specific maintenance rates under conditions of hydrogen or oxygen limitations. These values are similar to some obtained previously by Ohi et al. for another hydrogen bacterium. The observed increase in specific maintenance rates under hydrogen-versus-oxygen-limited culture may be associated with hydrogenase deactivation by oxygen.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260721
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  • 6
    Electronic Resource
    Electronic Resource
    Sex differences in blood protein patterns: Two dimensional electrophoretic analysis of the acidic proteins of rat plasma (1982)
    Weinheim : Wiley-Blackwell
    Electrophoresis 3 (1982), S. 52-58 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Plasma from normal male and female rats was analyzed, after removal of albumin by affinity chromatography, by two-dimensional electrophoresis. The results demonstrated gender differences in two plasma proteins with apparent molecular weights of 70 000 and 84 000 and isoelectric points ranging from 6.2 to 6.4, and 5.1 to 5.5, respectively. Both proteins were found to be more concentrated in the plasma minus albumin (P-alb) fraction of females than males in both Wistar and Sprague-Dawley rats. The 70 000 protein is serum albumin which was more completely removed from male plasma than female plasma. A possible mechanism responsible for this difference is discussed. The results support the existence of “maleness” and “femaleness” in blood protein patterns of rats.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150030110
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  • 7
    Electronic Resource
    Electronic Resource
    Demonstration of proteins in electrophoresis gels using radioactive stains (1984)
    Weinheim : Wiley-Blackwell
    Electrophoresis 5 (1984), S. 354-357 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We describe the use of 35S-labelled stains for the detection of submicrogram amounts of protein in SDS polyacrylamide gels. Many stains used to detect protein can be rendered radioactive, and proteins can be detected by radiofluorography due to stain binding in the absence of visible color. Of the stains tested. [35S]Ponceau Red S and [35S]Serva Blue R gave the best results. The potential utility of protein detection with radioactive stains is discussed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150050606
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  • 8
    Electronic Resource
    Electronic Resource
    The application of relatively large sample volumes to thin-layer polyacrylamide gels (1982)
    Weinheim : Wiley-Blackwell
    Electrophoresis 3 (1982), S. 231-232 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150030410
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