Publication Date:
1980-04-18
Description:
Extracellularly applied N-succinimidyl [2,3-3H]propionate was used in vivo to covalently label intra-axonal proteins in the rat sciatic nerve. This technique permitted a unique view of axonal transport of proteins independent of biosynthesis. The proteins detected in slow anterograde transport (1 to 2 millimeters per day) correspond to cytoskeletal proteins described in previous papers. The slowly retrogradely transported component (3 to 6 millimeters per day) was composed primarily of a single protein with a molecular weight of 68,000.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fink, D J -- Gainer, H -- New York, N.Y. -- Science. 1980 Apr 18;208(4441):303-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6154312" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Autoradiography
;
Axonal Transport
;
Axons/metabolism
;
Cytoskeleton/metabolism
;
Female
;
Histological Techniques
;
Molecular Weight
;
Nerve Tissue Proteins/*metabolism
;
Propionates
;
Rats
;
Sciatic Nerve/*metabolism
;
Succinimides
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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