ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
In chemical ionization mass spectrometry, the use of a noble reagent gas mixed with a proton transfer reagent gas is known to produce mass spectra which contain both protonated molecules and fragment ions. We have used a mixture of isobutane and argon to improve the mass spectra of peptides which have been derivatized to O-TMS-polyamino alcohols. The molecular weight is deduced from a prominent [M+H]+ ion; the sequence of amino acids is deduced from the usual A- and Z-series fragment ions. This technique enhances the sequence ions for lysine and glycine-containing peptides, and retains the ability to distinguish C-terminal leucine and isoleucine.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200101007
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