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  • 1
    Electronic Resource
    Electronic Resource
    I. Photoaffinity probes provide a general method to prepare synthetic peptide-conjugates (1984)
    Springer
    The protein journal 3 (1984), S. 465-478 
    Details
    ISSN: 1573-4943
    Keywords: photoaffinity probes ; synthetic peptide-conjugates ; solid-phase peptide synthesis ; benzophenone ; antibodies to synthetic peptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A general synthetic strategy is described for the preparation of peptide-conjugates where the peptides contain the NH2 terminal, COOH terminal, or internal regions of the protein sequence. Glycoprotein D of herpes simplex virus type 1 is used as a representative protein. Ten-residue peptide fragments of the native sequence were synthesized using standard solid-phase methodology. Photoprobes stable to conditions of synthesis and HF cleavage were coupled directly to the protected-peptide resin during synthesis. This one-step procedure eliminates the potential modification of functional groups in the sequence of interest that can occur when using chemically labile bifunctional reagents. Since the photoprobe is inert until photolysis, the synthetic peptide-probe can be readily purified by high-performance liquid chromatography before cross-linking to the carrier molecule. The following photoprobe derivatives were investigated: thep-azidobenzoyl,p-nitrophenylalanyl, andp-benzoylbenzoyl groups. The benzophenone photoprobes were shown to give the highest incorporation of peptide-probe with the protein carrier over a wide range ofpH and solvent conditions. For solid-phase synthesis three benzophenone photoprobes can be used: benzoylbenzoic acid, benzoylbenzoylglycine, andN e-(4-benzoylbenzoyl)-N α-t-butyloxycarbonyl-lysine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01025065
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  • 2
    Electronic Resource
    Electronic Resource
    II. Photoaffinity probes provide a general method to prepare peptide-conjugates from native protein fragments (1984)
    Springer
    The protein journal 3 (1984), S. 479-489 
    Details
    ISSN: 1573-4943
    Keywords: photoaffinity probes ; peptide-conjugates from native protein fragments ; benzophenone probes ; p-azidobenzoyl probes ; antibodies to protein fragments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The advantages of using amino-directed photoprobes to couple native fragments, obtained by enzymatic digestion with trypsin, to protein carriers to prepare peptide-conjugates is described. The following photoprobe reagents were investigated:N-hydroxysuccinimidylp-azidobenzoate,N-hydroxysuccinimidyl ester ofp-azidobenzoylglycine,N-hydroxysuccinimidylp-benzoylbenzoate, and pentachlorophenyl ester ofp-benzoylbenzoyl glycine or the symmetric anhydride ofp-benzoylbenzoylglycine. These reagents modify only the NH2-terminal amino group and/or COOH-terminal ε-amino group of lysine of the tryptic fragments. Since the photoprobe is inert until photolysis, the probe-modified native fragment can be readily purified by high-performance liquid chromatography before cross-linking to the carrier molecule. The benzophenone photoprobes were shown to give the highest incorporation of peptide onto the protein carrier.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01025066
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  • 3
    Electronic Resource
    Electronic Resource
    Repeated DNA sequences and species relatedness in the genusEquisetum (1983)
    Springer
    Plant systematics and evolution 143 (1983), S. 47-52 
    Details
    ISSN: 1615-6110
    Keywords: Equisetaceae ; Equisetum ; DNA reassociation kinetics ; DNA base composition ; C0t curves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The kinetics with which DNA reassociates and the thermal stability of rapidly reassociating (repetitive) DNA sequences have been monitored for six species in the genusEquisetum. Using the kinetic complexity for each of two repeated DNA sequence components as the basis for comparison, species in the subgenusEquisetum (E. arvense, E. fluviatile andE. telmateia) form one group and species in the subgenusHippochaete (E. hyemale, E. laevigatum andE. scirpoides) form another group. Using the difference in thermal stability between native and reassociated repetitive DNA for comparison, the same grouping of species is obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984111
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  • 4
    Electronic Resource
    Electronic Resource
    Phonoangiography: Qualitative and quantitative (1984)
    Springer
    Annals of biomedical engineering 12 (1984), S. 55-62 
    Details
    ISSN: 1573-9686
    Keywords: Carotid stenosis ; Bruit analysis ; Sound spectral analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Bruit analysis (phonoangiography) has been performed for many years as a method of characterizing arterial disease. Time displays of arterial bruits, particularly at the carotid bifurcation, have been used in an attempt to quantitate arterial narrowing. Despite the generalization that longer bruits and bruits which look and sound higher in frequency are often associated with severe disease, prospective studies have shown no useful predictive value for qualitative phonoangiography. In marked contrast, spectral bruit analysis or quantitative phonoangiography has been quite accurate in predicting the location and extent of carotid stenosis, and in distinguishing intrinsic from transmitted bruits. With this method, the peak systolic portion of the bruit is subjected to fast Fourier transform analysis. The peak frequency, beyond which amplitude drops as frequency increases further, is directly related to the residual lumen diameter of the stenotic common or internal carotid artery. Several blinded trials of this method have given results accurate to within 1 mm of angiographic values in 83–93% of cases studied. When used in conjunction with duplex doppler ultrasound scanning, 95% accuracy in diagnosis of patients with and without bruits may be achieved. This completely noninvasive method deserves more widespread use and may also be applicable to other cardiovascular sounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02410291
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular size and gel formation in branched poly(methyl methacrylate) copolymers (1980)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 181 (1980), S. 869-890 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Branched copolymers of methyl methacrylate with ethylene dimethacrylate or pentaerythritol tetramethacrylate with high weight average molecular weights Mw were prepared by free radical polymerization. The double bond conversion of these polymers (β) was measured dilatometrically and the gel point (βc) was located by extrapolation of bulk viscosity measurements. The polymers were studied by light-scattering, 1H NMR and viscometry. Hence it was possible to investigate the relationship between the molecular weight Mw and the reduced conversion quantity (1 - β/βc)-1 and also the molecular weight dependence of the z-average mean square radius of gyration 〈S2〉z. Considerable deviations from the classical Stockmayer theory but also from percolation theory were observed. 1H NMR showed a relative small extent of ring formation (10 to 24%) and a reduced apparent reactivity of the pendant double bonds, while the delay in the gel formation appeared to be caused by a much higher ring formation (80 to 90%). This apparent inconsistency is explained by a size-dependent ring formation, where the extent of ring formation is increased with the molecular dimensions; simultaneously, the pendant double bonds become progressively less accessible to cross-linking by growing radicals. The molecular weight dependence of 〈S2〉z is found to be in agreement with this heterogeneous reaction model.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1980.021810409
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  • 6
    Electronic Resource
    Electronic Resource
    State of the art in fermentation air filtration (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 844-847 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article presents several characteristics of a state-of-the-art fermentation air filter. The filter medium is composed solely of PTFE and has an absolute pore size rating of 0.2 μm. Quantitative bacteria and bacteriophage retention is shown based on live organism challenge tests. A nondestructive filter test, correlated to the microorganism challenge tests and called the Forward Flow Integrity Test, is described. This test has a sensitivity of one part in 1012 and can be performed in situ.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260805
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  • 7
    Electronic Resource
    Electronic Resource
    Kinetics of growth of the hydrogen-oxidizing bacterium Alcaligenes eutrophus (ATCC 17707) in chemostat culture (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 764-770 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The hydrogen-oxidizing bacterium, Alcaligenes eutrophus (ATCC 17707), was grown in chemostat culture with gas-phase (hydrogen, oxygen, and carbon dioxide) and liquid-phase (mineral nutrients) feedstreams; data were used to generate an analytical form for the specific growth rate equation. Model parameters obtained include Monod rate parameters for dissolved hydrogen and oxygen gases, yield coefficients, and specific maintenance rates under conditions of hydrogen or oxygen limitations. These values are similar to some obtained previously by Ohi et al. for another hydrogen bacterium. The observed increase in specific maintenance rates under hydrogen-versus-oxygen-limited culture may be associated with hydrogenase deactivation by oxygen.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260721
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  • 8
    Electronic Resource
    Electronic Resource
    Fast atom bombardment mass spectrometry of bradykinin and related oligopeptides (1981)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 8 (1981), S. 337-342 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The new technique of fast atom bombardment mass spectrometry has been applied to bradykinin, lysyl-bradykinin and methionyl-lysyl-bradykinin. High sensitivity was obtained at [M + H]+ in the positive ion spectra with a comparable response at [M - H]- in the opposite polarity. All spectra contained an abundance of structurally significant fragment ions. Schemes for relating the observed fragments to the known amino acid sequences are reported for all three molecules. Mass spectrometric investigations on bradykinin were followed by quantitative bioassays which showed that atom bombardment for ten minutes had no measurable effect on the biological activity of microgram samples.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200080804
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  • 9
    Electronic Resource
    Electronic Resource
    Fast atom bombardment mass spectrometry of cobalamines (1981)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 8 (1981), S. 492-495 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fast atom bombardment, a new method of ionizing molecules for mass spectrometry, has been found to be particularly applicable to molecules of an involatile nature. The mass spectra of vitamin B12 and related cobalamines are reported together with the mass spectrum of the coenzyme of vitamin B12. Fully resolved spectra, containing both molecular species and structurally significant fragmentation are reported for the first time.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200081005
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  • 10
    Electronic Resource
    Electronic Resource
    A new criterion of phase separation: The effect of diamine chain extenders on the properties of polyurethaneureas (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 27 (1982), S. 2167-2177 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A new criterion of phase separation of segmented polyurethaneureas. is found by comparing the relative crystallinity of the soft segment of polyurethaneurea. This criterion is more sensitive than the conventional thermal transition temperature measurements. The difference of crystallinity can be distinguished even if the thermal transition temperatures are the same. The polymers are synthesized by coupling a prepolymer consisting of poly(tetramethylene oxide) (PTMO) and 4,4′-diphenylmethane diisocyanate (MDI) with different chain extenders, ethylenediamine (ED), 1.6-hexanediamine (HD), and 4,4′-methylenedianiline (MDA). The degree of phase separation is found to relate to the structure of the chain extender and the hard-segment content as indicated by the relative crystallinity and initial modulus. Several explanations are proposed to discuss the relationship between these factors.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1982.070270627
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