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  • ASTROPHYSICS  (589)
  • ENERGY PRODUCTION AND CONVERSION  (379)
  • Cell & Developmental Biology  (283)
  • INSTRUMENTATION AND PHOTOGRAPHY  (277)
  • 1980-1984  (1,528)
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  • 1
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this study, the intracellular concentrations of six elements (mmole/kg dry weight) were directly measured in the muscle fibers of pectoralis major muscles of eight week old, genetically dystrophic and normal chickens by the X-ray microanalysis technique. The extent of muscle degeneration was evaluated by morphometric measurements of muscle fiber diameter and other histological changes. A significant increase in the concentration of intracellular sodium and chlorine was evident in dystrophic muscles. The concentration of intracellular sodium was 127.0 ± 35.0 in the muscle fibers of dystrophic chicks compared to 65.7 ± 16.5 in normal controls. The concentration of chlorine was 90.5 ± 27.5 and 54.1 ± 5.5 in the muscle fibers of dystrophic and normal chicks respectively. The intracellular concentrations of potassium, magnesium, phosphorous, and sulfur remained unchanged in the dystrophic condition. Morphometric studies revealed that the dystrophic pectoralis muscles contain fewer but thicker fibers per unit area compared to normal pectoralis muscles. The importance of these findings are discussed in relation to the results of earlier investigations.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2011-08-18
    Description: A lidar instrument based on pulsed frequency-doubled carbon-dioxide lasers has been used at 4.88 microns for remote sensing of atmospheric carbon dioxide. A tunable-diode laser spectrometer provided the high-resolution spectroscopic data on carbon-dioxide line strength and line broadening needed for an accurate differential absorption measurement. Initial field measurements are presented, and instrument improvements necessary for accurate carbon dioxide measurement are discussed.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Applied Optics (ISSN 0003-6935); 22; Sept. 1
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  • 3
    Publication Date: 2014-09-13
    Description: A three factor (spectral, spatial, and radiometric resolution), two level (TM and MSS) analysis of variance (ANOVA) approach allowed evaluation of the effects of each factor individually and in all possible combinations. Digital classification accuracy was used as the figure of merit. Nine study sites in Washington, D.C. each of approximately 256 x 256 TM pixels, were randomly selected from the full scene for analysis. These results strongly suggest that the quantization level improvements and the addition of new spectral bands in the visible and middle IR regions (both afforded by the TM sensor design) can result in improved capabilities to accurately delineate land cover categories using a per point Gaussian maximum likelihood classifier. On the other hand, results indicate that the increase in spatial resolution to 30m does not significantly enhance classification accuracy. The spatial result points to an inherent limitation of a per point classifier and to the need to improve data analysis techniques to handle high spatial resolution data.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: LANDSAT-4 Sci. Invest. Summ., Including Dec. 1983 Workshop Results, Vol. 2; p 93-97
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 307-315 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The Ca2+ activation mechanism of the longitudinal body wall muscles of Parastichopus californicus (sea cucumber) was studied using skinned muscle fiber bundles. Reversible phosphorylation of the myosin light chains correlated with Ca2+-activated tension and relaxation. Pretreatment of the skinned fibers with ATPγS and high Ca2+ (10-5M) resulted in irreversible thiophosphorylation of the myosin light chains and activation of a Ca2+ insensitive tension. In contrast, pretreatment with low Ca2+ (10-8M) and ATPγS results in no thiophosphorylation of the myosin light chains or irreversible activation of tension. These results are consistent with a Ca2+-sensitive myosin light chain kinase/phosphatase system being responsible for the activation of the muscle. Other agents known to have an effect upon the Ca2+-activated tension in skinned vertebrate smooth muscle fibers (trifluoperazine, catalytic subunit of the cyclic AMP-dependent protein kinase, and calmodulin) did not have an effect on myosin light chain phosphorylation or Ca2+-activated tension. These results suggest a different type of myosin light chain kinase than is found in vertebrate smooth muscle is responsible for the activation of parastichopus longitudinal body wall muscle.
    Additional Material: 9 Ill.
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  • 5
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
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  • 6
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    In:  CASI
    Publication Date: 2014-09-16
    Description: Observations at the 2.6 mm line of CO reveal the presence of a large number of molecular clouds at high galactic latitude. If the velocity dispersion of the clouds is a measure of their scale height, the mean distance of the ensemble detected is 100 pc. The clouds are unusual in that either they are not gravitationally bound or they are very deficient in CO relative to molecular hydrogen. These clouds represent a heretofore unrecognized component of the local interstellar medium. If they are pervasive in the Milky Way, they probably represent the small molecular cloud component of the interstellar medium.
    Keywords: ASTROPHYSICS
    Type: NASA. Goddard Space Flight Center Local Interstellar Medium, No. 81; p 231-234
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  • 7
    Publication Date: 2019-06-28
    Description: Morphological evidence is presented (in the form of 3-A-bandpass Fabry-Perot images made with a CCD camera) that the two bright emission-line systems seen toward the galaxy NGC 1275 arise from a high-velocity impact of a foreground galaxy upon the accretion flow of gas cooling in the center of the Perseus cluster. The uniquely high optical-line luminosity of NGC 1275, in comparison with other central galaxies in clusters observed to have cooling flows, may be explained by energy deposited during the collision. Using additional information from 21-cm and extinction measurements, a rough model of the interaction is developed. Problems remain with this model - such as the likelihood of a gas-rich system penetrating to the cluster center. The kinematic structure of the optically emitting gas shows additional complex structure near the nucleus of NGC 1275.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 2 - Letters to the Editor (ISSN 0004-637X); 275; L27-L31
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  • 8
    Publication Date: 2019-06-28
    Description: The Einstein Imaging Particle Counter observed a major X-ray flare in its entirety during a 5-hr period of simultaneous observations, with the IUE, of the dM5e flare star Proxima Centauri in August, 1980. The detailed X-ray light curve, temperature determinations during various intervals, and UV line fluxes obtained before, during, and after the flare indirectly indicate a 'two-ribbon flare' prominence eruption. The calculated ratio of coronal to bolometric luminosity for the event is about 100 times the solar ratio. The Proxima Cen corona is analyzed in the context of static loop models, in light of which it is concluded that less than 6% of the stellar surface seems to be covered by X-ray emitting active regions.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal; vol. 267
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  • 9
    Publication Date: 2019-06-28
    Description: The 1.24-sec pulsations of Her X-1 in the energy range 13-75 keV have been analyzed in data obtained from the UCSD/MIT experiment on HEAO 1 during observations of the source on three dates in February 1978 and three in August 1978. Observational results are (1) the main pulse broadens somewhat with increasing energy; (2) the pulsation light curve undergoes pronounced changes at the leading edge of the main pulse from day to day; (3) spectral hardening within the main pulse is confirmed; (4) a 40-60 keV spectral feature in the spectrum is confirmed; (5) this feature is resolved, the pulse width broadening is greater than 20%, and its centroid varies with pulsation phase; and (6) the 13-75 keV spectrum does not noticeably vary from day to day, except for an overall intensity factor. Some implications of these results for the prevailing models of Her X-1 and the HZ Her-Her X-1 system are briefly discussed.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal; vol. 240
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  • 10
    Publication Date: 2019-06-28
    Description: An enclosure provides a containerless environment in which a sample specimen is positioned. The specimen is heated in the containerless environment, and the specimen melt is dropped through the tube in which it cools by radiation. The tube is alternatively backfilled with an inert gas whereby the specimen melt cools by both radiation and convection during its free fall. During the free fall, the sample is in a containerless, low-gravity environment which enhances supercooling in the sample and prevents sedimentation and thermal convection influences. The sample continues to supercool until nucleation occurs which is detected by silicon photovoltaic detectors. The sample solidifies after nucleation and becomes completely solid before entering the detachable catcher. The amount of supercooling of the specimen can be measured by knowing the cooling ratio and determining the time for nucleation to occur.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Format: application/pdf
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