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  • Pisum  (2)
  • ASTRONOMY  (1)
  • Enzyme degradation  (1)
  • 1H-13C COSY NMR
  • PIC
  • 1980-1984  (4)
  • 1
    Electronic Resource
    Electronic Resource
    The initiation of legumin synthesis in immature embryos of Pisum sativum L. grown in vivo and in vitro (1980)
    Springer
    Planta 149 (1980), S. 454-460 
    Details
    ISSN: 1432-2048
    Keywords: Embryo culture ; Enzyme-linked immunosorbent assay (ELISA) ; Legumin ; Pisum ; Protein (storage) ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly sensitive immunoassay has been used for the detection of a major storage protein, legumin, in embryos of Pisum sativum L.; with this technique nanogram quantities could be measured. In the two varieties tested, legumin could be detected in embryos in vivo, when they had attained a fresh weight of 2·10-3 g and 3·10-3 g, respectively. Contrary to earlier claims, embryos cultured in vitro were shown to be capable of initiating legumin synthesis. This capacity to initiate legumin synthesis was confirmed by two-dimensional isoelectric focusing-electrophoresis and fluorography; embryos harvested before initiation of legumin synthesis and cultured in radioactive medium were shown to have synthesized legumin subunits. The amounts of legumin and total protein synthesized per unit fresh weight were consistently greater in vitro than in equivalent embryos grown in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385747
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The molecular basis of the selectivity of protein degradation in stressed senescent barley (Hordeum vulgare cv. Proctor) leaves (1983)
    Springer
    Planta 158 (1983), S. 550-559 
    Details
    ISSN: 1432-2048
    Keywords: Enzyme degradation ; Hordeum (protein degradation) ; Protein degradation ; Senescence ; Stress and protein degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The molecular basis for the selectivity of protein degradation has been examined in osmotically stressed and senescent barley leaves. Relatively weak correlations between the in-vivo rate of loss of enzyme activity, and the charge and molecular weight of the enzymes ahve been detected. We interpret these correlations as supporting the view that the selectivity of enzyme degradation is the result of the physical properties of the enzymes being degraded. The weakness of the correlates is taken to mean that a number of properties which contribute to the selectivity are independent of one another. Under in-vitro conditions (autolysis at 0° C), the loss of enzyme activity was weakly correlated with the charge of the enzymes. However, there was a general similarity between the in-vivo pattern of loss of enzyme activity and the in-vitro patterns under a number of conditions. Furthermore double-isotope experiments demonstrated that the in-vivo degradation of soluble protein was reflected by in-vitro degradation under a number of conditions. Consequently we conclude that the selectivity of protein degradation is largely independent of the nature of the proteolytic system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397246
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Pollen irradiation and the transfer of maternal genes in Pisum sativum (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 245-248 
    Details
    ISSN: 1432-2242
    Keywords: Pollen ; Irradiation ; Gene transfer ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pollen of Pisum sativum was exposed to doses of 900 to 6,000 r of X-rays prior to pollinating a multiply marked genotype. The first generation progeny closely resembled that produced with unirradiated pollen. In the second generation, five loci were monitored, and the results showed that irradiation enhanced the proportion of maternal information transmitted to the progeny; the practical implications of the data, as well as the mechanism underlying the effect are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317046
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  • 4
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    Direct imaging of planetary systems around nearby stars (1980)
    In:  Other Sources
    Details
    Publication Date: 2019-06-27
    Description: It is shown that by utilizing a space-borne telescope it may be possible to directly image planetary systems around the nearest stars. Direct imaging, while limited to the nearest stars, would provide a great deal of information on the planet, over and above the planet's orbital elements - estimates of the planet's size and rotation rate, and the presence or absence of an atmosphere are all possible if light from the planet can be separated from light from the star. It is shown that a Jupiter-like planet would be detectable around several of the nearest stars.
    Keywords: ASTRONOMY
    Type: Icarus; 42; Apr. 198
    Format: text
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    NASA TECHNICAL REPORTS
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