ISSN:
1432-2048
Keywords:
Chlamydomonas (mutants)
;
Nitrate reductase
;
Xanthine dehydrogenase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Wild-type Chlamydomonas reinhardii cells have xanthine dehydrogenase activity when grown with nitrate, nitrite, urea, or amino acid media. Mutant strains 102, 104, and 307 of Chlamydomonas, lacking both xanthine dehydrogenase and nitrate reductase activities, were incapable of restoring the NADPH-nitrate reductase activity of the mutant nit-1 of Neurospora crassa, whereas wild type cells and mutants 203 and 305 had xanthine dehydrogenase and were able to reconstitute the nitrate reductase activity of nit-1 of Neurospora. Therefore, it is concluded that in Chlamydomonas a common cofactor is shared by xanthine dehydrogenase and nitrate reductase. Xanthine dehydrogenase is repressed by ammonia and seems to be inessential for growth of Chlamydomonas.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00383895
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