ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The photosynthetic N2-fixing cyanobacterium Anabaena ATCC 27893 was immobilized by entrapment in beads of calcium alginate gel and used in a three-phase fluidised bed reactor for NH 4 + production from air, light and water. NH 4 + release from the beads was induced by adding L-methionine-DL-sulphoximine (MSX) which inhibits glutamine synthetase, the primary NH 4 + assimilating enzyme. The morphological features of the beads and entrapped cyanobacterium have been examined by light and scanning electron microscopy before, during and after the period of NH 4 + production. The cyanobacterium appeared morphologically normal throughout the 792 h experimental period with its filamentous form and heterocyst content being sustained throught. Our results indicate that Anabaena ATCC 27893 is well able to withstand prolonged entrapment and varying experimental conditions while entrapped.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00499865
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