ISSN:
1432-072X
Keywords:
Ectothiorhodospira shaposhnikovii
;
Bacterial ferredoxin
;
High potential iron sulfur protein (HIPIP)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In addition to several cytochromes three iron sulfur proteins were detected in mixotrophically grown cells of Ectothiorhodospira shaposhnikovii, a member of the Chromatiaceae. They were identified as a bacterial ferredoxin and two high potential iron sulfur proteins (HIPIPs). The two HIPIPs were purified and characterized. They were named according to their differing retention times on a DEAE-cellulose column using a continuous NaCl gradient: “early” and “late” HIPIP. The HIPIPs contain 4 mol of non-heme iron and 4 mol of acid labile sulfur per mol protein. Under the conditions of purification the “early” HIPIP (E m, 7+270 mV) was present in a semi-reduced state. Using ion-exchange chromatography the “early” HIPIP could be split into a reduced green-brown (pI=3.7) and an oxidized red-brown (pI=3.9) fraction. The “late” HIPIP (pI=3.8) showed a midpoint potential of only+155 mV, the lowest redox potential of a HIPIP described so far.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00414556
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