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  • Hindawi  (2)
  • Wiley-Blackwell  (1)
  • Wiley
  • 1980-1984  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 6 (1982), S. 121-125 
    ISSN: 0148-7280
    Keywords: histidine decarboxylase ; preimplantation embryo ; blastocyst ; compaction ; histamine ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The present study determines the effect of a specific and an irreversible inhibitor of histidine decarboxylase (HDC), α-fluoromethylhistidine (α-FMH) on the mouse preimplantation embryo development in vitro. The embryo culture technique was used to assess the effect of α-FMH. Embryos recovered at 0800-0900 hr (AM) on day 3 of pregnancy were 4-8 cells, whereas those recovered at 1600-1630 hr were mostly 8-cell compacted embryos. Of the day 3-AM embryos, 81.3 ± 4.3% developed to blastocysts within 48 hr when cultured in the medium alone, but addition of α-FMH (0.19 or 0.38 mM) drastically reduced the blastocyst formation to 26.6 ± 7 or 16.8 ± 4.3%. Most of them were arrested before the compaction stage. Addition of L-histidine, the substrate for HDC, did not alter the inhibition of blastocyst formation in the presence of α-FMH (37.2 ± 10.9%). Of the day 3-PM embryos, 99.3 ± 0.7% developed to blastocyst stage when cultured in the medium alone and addition of α-FMH (0.19 or 0.38 mM) did not affect the embryo development (92.1 ± 4.3 or 81.9 ± 9.9% developed to blastocysts). The birth of healthy young following transfer of these blastocysts into pseudopregnant mice indicates normal development of the embryos under this condition. The results suggest that histamine synthesis may be required for the process of compaction and thus the formation of blastocyst.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 1981-01-01
    Description: In the references [1, 2, 3] a perturbed iterative scheme (PIS) has been studied both theoretically and computationally to solve nonlinear equations. In this article a more general analysis of its convergence properties has been done
    Print ISSN: 0161-1712
    Electronic ISSN: 1687-0425
    Topics: Mathematics
    Published by Hindawi
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  • 3
    Publication Date: 1980-01-01
    Description: A numerical algorithm dealing with solutions of equations with one variable may not be extended to solve nonlinear systems withnunknowns. Even when such extensions are possible, properties of these two similar algorithms are, in general, different. In [2] a perturbed iterative scheme (PIS) has been developed to solve nonlinear equations with one variable. Its properties with regard to nonlinear systems were analyzed in [1]. Here these properties were extended ton-coupled nonlinear systems.
    Print ISSN: 0161-1712
    Electronic ISSN: 1687-0425
    Topics: Mathematics
    Published by Hindawi
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