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  • Trifolium repens  (2)
  • Springer  (2)
  • Cambridge University Press
  • National Academy of Sciences
  • Oxford University Press
  • Periodicals Archive Online (PAO)
  • 1980-1984  (2)
Collection
Keywords
Publisher
  • Springer  (2)
  • Cambridge University Press
  • National Academy of Sciences
  • Oxford University Press
  • Periodicals Archive Online (PAO)
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Biochemical characterisation of the Li locus, which controls the activity of the cyanogenic β-glucosidase in Trifolium repens L. (1982)
    Springer
    Plant molecular biology 1 (1982), S. 169-181 
    Details
    ISSN: 1573-5028
    Keywords: cyanogenesis ; β-glucosidase ; linamarase ; Trifolium repens ; null loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cyanogenic β-glucosidase (linamarase) was purified from white clover leaf tissue. The enzyme is a homodimer with a molecular weight of 105 300–103 400 daltons estimated from molecular exclusion chromatography. The effect of buffer ions on the pH optimum and charge properties of the enzyme are presented. A combination of molecular exclusion chromatography and CM cellulose ion exchange chromatography purified linamarase 16 fold to a single 62 000 dalton polypeptide on SDS polyacrylamide gel electrophoresis. This polypeptide represented about 5% of the total soluble leaf protein and can be seen as a prominent band in SDS polyacrylamide gel electrophoresis of crude leaf extracts from Li Li plants. Screening backcross progeny showed that extracts from li li plants, which have no linamarase activity, lack this 62 000 dalton polypeptide. Linamarase is the major glycoprotein in white clover leaf extracts which binds to Concanavalin A-Sepharose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021030
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A study of dominance at the locus controlling cyanoglucoside production in Trifolium repens L. (1982)
    Springer
    Euphytica 31 (1982), S. 477-483 
    Details
    ISSN: 1573-5060
    Keywords: Trifolium repens ; white clover ; cyanogenesis ; cyanoglucosides ; linamarin ; lotaustralin ; dominance ; gene dosage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Ac allele for cyanoglucoside production in white clover has been shown to be incompletely dominant and a dosage effect is indicated at this locus. The recessive allele, ac, when homozygous gives rise to absence of cyanoglucosides in the leaf tissue, whereas in heterozygous plants it has been shown to reduce the level of cyanoglucoside, to a level less than half the value found in Ac Ac plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021666
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    Paper (German National Licenses)
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