ALBERT

Notes: Desorption isotherms at 25°C and 43°C and adsorption isotherms at 25°C were determined for salted and unsalted female roe of mullet (Mugil cephalus). Isotherms for ground white muscle of mullet were also determined for desorption at 25°C, 43°C, and 54.5°C, and for adsorption at 25°C. Adsorption-desorption hysteresis was evident for the sigmoid shaped isotherms. At 45°C and 15% RH, and also at 45°C and 25% RH, mullet fillets dry almost four times faster than mullet roe. An air temperature lower than 45°C should be used to obtain an acceptable color for the roe.〈section xml:id="abs1-2"〉〈title type="main"〉SUMMARYDESORPTION AND ADSORPTION isothems at several temperatures were determined for salted and unsalted female roe of mullet, and ground white muscle of mullet. All isotherms are sigmoid shaped, typical of isotherms for food products. Adsorption-desorption hysteresis was also evident for salted and unsalted roe, and for ground white muscle. At any given temperature and relative humidity, the equilibrium moisture content for unsalted roe was always lower than that for salted roe.Under the conditions tested, mullet fillets dried almost four times faster than roe. Roe placed in an air stream did not dry faster than roe in still air at the same temperature and relative humidity. This indicated that the restriction to moisture removal was in the moisture migration within the roe rather than at the roe surface. Air temperature should be lower than 45°C to prevent excessive browning of the row. Browning began on the tips and continued toward the center of the roe.

Notes: The effects of ultraviolet radiation (λ= 254 nm) on the kinetics of encystment of the hypotrichous ciliate Laurentiella acuminata and the structure of resting cysts obtained from irradiated precystic cells are reported. High doses of UV-radiation caused a delay of encystment with a linear increase in the average time for obtaining 50% of encystment (EN50). Resting cysts with abnormal cyst walls were obtained when precystic cells were irradiated in the exposure range 720 to 960 J/m2. The cystic layer (mesocyst) was approximately twice as thick (6.5 μ m) as normal (3.7 μ m). Microscopical observations of abnormal cysts revealed the presence of two complete mesocysts, and the absence of the spines characteristic of the ectocyst. The UV-dependent effects on the cyst wall were gradually corrected in successive generations of the irradiated cells.

Notes: Morphogenetic events during division, physiological reorganization, and postraumatical regeneration, the last being induced both chemically and microsurgically, were studied by light microscopy on protargol-impregnated specimens of the hypotrichous ciliate, Laurentiella acuminata. Parakinetal stomatogenesis, from transverse cirrus-1 during division and reorganization, changes during regeneration to a parakinetal one which characterizes more primitive members of Hypotrichida in the S. O. Stichotrichina, but solely when the AZM is damaged. These morphogenetic events a) confirm the previous inclusion of L. acuminata among the Oxytrichidae on the basis of its morphological characters and indicate that it is a primitive species of this family related with the Stichotrichina through genera Pleurotricha and Paraurostyla; b) suggest a synthetic model that explains both the positioning and timing of cortical morphogenesis in the cell cycle. The key point of this model is the attribution to the AZM of a repressive capacity on the stomatogenic area, the last one being positioned according to the system of gradients of morphogenetic activity proposed by Jerka-Dziadosz to explain location of primordia in urostylids. This repression is manifested not as a gradient, as indicated by De Terra, but as a long-term repression limited to a certain distance. Simultaneous repression and stimulation occurring in a growing cortex with the AZM remaining constant in size could explain the critical ratio, buccal cortex/somatic cortex, at which stomatogenesis is triggered as indicated by De Terra.

Notes: The encystment of Laurenliella acuminata was divided into five stages: stage A (precystic semitransparent cell with dark-globules), stage B (precystic transparent cell), stage C (precystic pigmented cell), stage D (spherical shape without cyst wall) and stage E (young resting cyst), on the basis of observations of changes in morphology and pigmentation during encystment. The duration of these stages was also established. Observations by electron microscopy confirmed that the cyst wall, composed of four layers, is derived from different kinds of precursors which are synthesized “de novo.” The ectocyst precursors are composed of stacks of between 5 and 12 small thin plates or discs; these stacks are about 0.9 μm in length and 0.06 μm in height. The mesocyst precursors are fibrillar bodies of variable shapes, about 2.4 μm in maximum length and 0.12–0.16 μm in diameter. These precursors appear in the cytoplasm of the precystic cell during the first precystic stage (stage A). The endocyst precursors are rounded bodies surrounded by a fine membrane, and their contents appeared similar to the endocyst. The granular layer precursors are spherical bodies about 0.1–0.2 μm in diameter, surrounded by a double membrane presenting ribosomes adhering to its outer membrane. Both endocyst and granular layer precursors are observed in the precystic cytoplasm from stage B. On the basis of ultrastructural studies, a formation and growth model of the cyst wall of the hypotrichous ciliate Laurentiella acuminata is proposed.

Notes: RESUME. Chacun des 45–80 organelles adoraux de Bursaria truncatella O. F. Müller est constitué de 3 rangées de cinétosomes et l'aire buccale droite est couverte de nombreuses doubles rangées de cinétosomes. La stomatogenèse débute par la désorganisation et la résorption des organelles buccaux postérieurs. Puis, il y a désorganisation des rangées parorales de cinétosomes et multiplication des cinétosomes sur l'aire orale droite, en měme temps que sont rompues, selon une ligne oblique, un certain nombre de cinéties somatiques. La prolifération des cinétosomes aux extrémités des cinéties. de part et d'autre de la ligne de rupture, aboutit, d'une part, à la formation d'un champ anarchique qui est le primordium oral droit de l'opisthe, d'autre part, à la formation de nombreux doublets qui constituent chacun le primordium de chaque organelle adoral. Après la séparation des tomites, les cinétosomes de l'aire droite s'ordonnent en doubles rangées et les organelles adoraux se complètent par addition d'une 3ème rangée de cinétosomes. Les cinétosomes somatiques sont jumelés, reliés par 2 desmoses. Les fibres transverses postérieures et les fibres postciliaires forment de longs rubans de microtubules dirigés vers l'arrière et juxtaposés dans les crětes intercinétiennes. Les doubles rangées droites de cinétosomes buccaux sont assimilables à des stichodyades. Les organelles des cinétosomes adoraux portent des rideaux de fibres postciliaires convergents ou divergents. La rangée postérieure de chaque organelle est non ciliée. Par son type de stomatogenèse, par sa structure corticale, par l'ultrastructure des organelles adoraux, Bursaria appartient aux Colpodidea, ce qui suggère des remarques de plusieurs types.SYNOPSIS. In Bursaria truncatella O. F. Müller, each of the 45–80 adoral organelles is composed of 3 rows of kinetosomes, and the right buccal area is covered by many double rows of kinetosomes. Stomatogenesis begins by disorganization and disappearance of the posterior buccal organelles. Next, there is disorganization of the paroral rows of kinetosomes and multiplication of kinetosomes in the right oral area; at the same time, some somatic kineties are disrupted along an oblique line. Multiplication of kinetosomes at the extremities of the kineties, on both sides of the disruption, leads to the formation of an anarchic field which is the right oral primordium of the opisthe and the formation of doublets each of which constitutes an adoral organelle. After the separation of the tomites. the kinetosomes in the right buccal area position themselves, and the adoral organelles are completed by the addition of a 3rd row of kinetosomes. Somatic kineties are formed by successive pairs of ciliated kinetosomes united by 2 desmoses. the long posterior transverse ribbons and the postciliary ribbons extend posteriad, overlapping in the pellicular ridges. Oral rows of kinetosomes on the right can be compared with stichodyads. the adoral kinetosomes have convergent or divergent postciliary ribbons. the posterior row of kinetosomes in each organelle is not ciliated. By the type of stomatogenesis, the cortical ultrastructure, the ultrastructure adoral of its organelles, Bursaria belongs to the Colpodidea.

Notes: RESUME.Après résorption des structures buccales du protomonte, la stomatogenèse ne commence que sur les produits de l'avant-dernière division du tomonte. Elle se poursuit et se complète sur les tomites individualisés de la dernière division. Au niveau des extrémités antérieures des cinéties somatiques intercalaires et des extrémités rompues de cinéties bipolaires, une lère vague de proliferation des cinétosomes produit de courtes lignes, obliques, de cinétosomes isolés. Elles se transforment en lignes d'une double rangèe de cinétosomes, à la suite d'une 2ème vague de multiplication de cinétosomes. Un alignement de ces segments, procédant de gauche à droite et d'avant en arrière, constitue successivement les 3 membranelles longitudinales en doublets (M1 puis M2 et M3) ainsi que la membrane parorale, également en doublet. Une 3ème vague de proliferation cinétosomienne juxtapose une rangée de cinétosomes à droite des doublets (sauf à l'extrémité postérieure de M1 et au niveau de la parorale) transformant les promembranelles en triplets. Cette proliferation cinétosomienne se prolonge par addition d'une 4ème rangée de cinétosomes au trajet médian et postérieur de M2 et peut-ětre à M3 et par juxtapositions successives de nouvelles rangées supplémentaires à l'extrémité postérieure de M2 (flamme). Les cinétosomes des rangées droites de promembranelles portent de larges rideaux de nombreuses fibres postciliaires. Les cinétosomes des autres rangées de M2, au moins, ont également des fibres postciliaires. Entre les cils de promembranelles il n'y a pas de couche alvéolaire, ni d'épiplasme. Une résorption des cinétosomes commence à se manifester par disparition des cinétosomes de la rangée gauche de la parorale dont subsistent les cinétosomes droits porteurs de fibres postciliaires. A vec le raccourcissement de l'aire buccale la résorption s'étend aux cinétosomes postérieurs des 2 rangées droites de M1, et des extrémités antérieures des 2 (ou 3?) rangées droites dc M3. Une invagination de la dépression buccale entraǐne vers la gauche les organelles buccaux et enfonce les cinéties vestibulaires en remontant en avant et à gauche leurs extrémités postérieures tronquées. Il y a régression postéro-antérieure totale des cinétosomes de la parorale. M1 reste constituée au départ de 3 rangées ciliaires; M2 est également formée de 3 rangées ciliaires doublées postérieurement de nombreuses rangées constituant la flamme; M3 n'est finalement constituée que d'une seule rangée ciliaire. Une ultime proliferation cinétosomienne aux extrémités antérieures de cinéties vestibulaires serait peut-ětre à l'origine d'un champ allongé de nouveaux cinétosomes vestibulaires.〈section xml:id="abs1-1"〉〈title type="main"〉ABSTRACTAfter resorption of the buccal structures of the protomont, stomatogenesis begins only in the products of the penultimate division of the tomont. It continues to completion in the individualized tomites of the last division. At the anterior ends of the intercalary somatic kineties and the broken ends of bipolar kineties, a first wave of kinetosome proliferation produces short streak lines of isolated kinetosomes. These develop into lines formed of double rows of kinetosomes following a second wave of kinetosome multiplication. An alignment of these segments, proceeding from left to right and from front to rear, constitutes successively the three longitudinal membranelles in doublets (M1 then M2 and M3), and the paroral membrane, also a doublet. A third wave of kinetosome proliferation juxtaposes a row of kinetosomes to the right of the doublets (except at the posterior end of M1 and at the level of the paroral membrane) to give triplets. This proliferation is extended by addition of a fourth row of kinetosomes on the median and posterior path of M2 and perhaps M3, and by successive juxtaposition of further rows at the posterior end of M2 (flare). The kinetosomes of the right hand rows of promembranelles bear wide ribbons of numerous postciliary fibers. There is no alveolar layer nor epiplasm between the cilia of the promembranelles. Resorption of kinetosomes begins by disappearance of the kinetosomes of the left hand row of the paroral membrane; the right hand kinetosomes carrying postciliary fibers remain. With shortening of the buccal zone, resorption extends to the kinetosomes of the two posterior rows of M1 and the anterior ends of the two (or three?) left hand rows of M3. An invagination of the buccal cavity draws the buccal organelles to the left and pushes in the vestibular kineties while raising forward and to the left their truncated posterior ends. Total postero-anterior regression of the kinetosomes of the paroral membrane occurs. Membranelle 1 remains composed of three rows of cilia; M2 is also composed of three rows of cilia edged posteriorly by numerous rows constituting the flare; M3 is composed of a single row of cilia. A final kinetosome proliferation at the anterior ends of the vestibular kineties might be responsible for the extended field of new vestibular kinetosomes.

Notes: In five species of fish from the Family Sciaenidae, collected from marine, brackish and fresh-water environments, the following parameters were studied: haemoglobin concentration, haematocrit, number of red blood cells, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, blood pH, oxygen affinity of blood and stripped haemolysate, Root effect, the number of haemoglobins separated by polyacrilamide electrophoresis, iron concentration and osmotic pressure of the serum. Plagioscion squamosissimus, the only freshwater species studied, clearly separated from the other four species; it exhibited the highest haemoglobin number, haemotocrit, number erythrocytes, of oxygen affinity of the haemolysate, and the lowest oxygen affinity of the blood, iron concentration, pH and osmotic pressure.

Notes: Alien H-2k-like antigens were found to be expressed by a methylcholanthrene induced tumour of BALB/c (H-2d) origin. H-2 specificities of the k haplotype were detected on this tumour by a variety of serological techiques, including 51Cr-release cytotoxicity, microradioassay and absorption. The antisera employed were conventional polyspecific alloantisera, typing sera with restricted specificty and monoclonal hybridoma-derived anti-H-2k antibodies.The tumour has a low expression of the private specificty 31, which characterizes Kd molecules, and does not seem to express the private specificities of Dd, Kk and Dk molecules. It appears to express predominantly alien H-2-like antigens which are very similar to but not identical with normal H-2k molecules.

Notes: Abstract The antifungal antibiotic papulacandin β inhibited B(1,3)glucan-synthase activity, in vitro, from Schizosaccharomyces pombe. Levels of β(1,3)glucan-synthase from antibiotic-treated cultures were lower than the control cultures whereas mannan-synthase and β(1,3)glucanase activities were almost unaffected. The presence of an osmotic stabilizer reduced the inhibition of growth caused by the antibiotic. Addition of papulacandin β to a culture of S. pombe specifically inhibited incorporation of glucose into the β-glucan cell wall fraction. The fatty acids as well as the hydroxyl groups on the phenol residue of the papulacandin β molecule were essential for the inhibitory activity.