Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
The @journal of eukaryotic microbiology
30 (1983), S. 0
ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
The heliozoan Echinosphaerium nucleofilum produced about 75 times 103 floating cells per 19-cm culture dish per day when fed the green flagellate Chlorogonium elongatum. This method yields enough cells for usable quantities of subcellular fractions. Heliozoa were lysed in a detergent mixture containing stabilizing reagents, and axonemal bundles of axopodial microtubules were isolated from the lysate by differential centrifugation. Polyacrylamide gel electrophoresis in sodium dodecylsulfate showed two prominent bands tentatively designated alpha- and beta-tubulin. Apparent molecular weights were 51.8 times 103 and 48.1 times 103, respectively. As assayed by electron microscopy of negatively stained whole mounts, the microtubule bundles splintered readily, although glycerol tended to inhibit this fraying. Intermicrotubule bridges could be observed in some axonemal splinters.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.1983.tb02933.x
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