ISSN:
1432-072X
Keywords:
Bacillus thuringiensis
;
Plasmid pC194
;
Transformation
;
Plasmid transfer
;
Protoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Staphylococcus aureus plasmid pC194 which codes for resistance to chloramphenicol was introduced into six Bacillus thuringiensis strains representing five varieties by protoplast transformation. Six other varieties could not be transformed. pC194 could be identified in transformed strains as autonomous plasmid. The transformed clones contained in addition a new extrachromosomal element of somewhat lower electrophoretic mobility hybridizing with pC194, and pC194 in multimeric forms. pC194 was also transferred from one B. thuringiensis variety to another and from Bacillus thuringiensis to Bacillus subtilis and vice versa by a conjugation-like process, requiring close cell-to-cell contact.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00402002
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