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  • Electron microscopy  (2)
  • 21.10.Re
  • Springer  (2)
  • American Institute of Physics
  • Nature Publishing Group
  • Springer Nature
  • 1980-1984  (2)
Collection
Keywords
Publisher
  • Springer  (2)
  • American Institute of Physics
  • Nature Publishing Group
  • Springer Nature
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Freeze fracture studies of reaction centers from Rhodospirillum rubrum in chromatophores and liposomes (1981)
    Springer
    Archives of microbiology 130 (1981), S. 125-128 
    Details
    ISSN: 1432-072X
    Keywords: Rhodospirillum rubrum ; Chromatophores ; Reaction centers ; Liposomes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In freeze-fractures of chromatophores of Rhodospirillum rubrum the reaction centers are seen as hexagonal arranged particles of 13 nm diameter with a density of around 5,500 particles per μm2. Similar regions on the cytoplasmic membrane suggest that these parts are the prospective invagination sites. Isolated reaction centers are easily incorporated into liposomes. In freeze fractures of liposomes particles similar in shape and size, although less dense as in chromatophores are observed. In negative staining much smaller units of only 5 nm in diameter are found indicating that reaction centers occur in the membrane as tri- or tetramers. There is a strong correlation between particle density in chromatophores and titratable reaction centers remaining in these membranes after extraction of reaction centers by detergents; both values are in good agreement with the yield of reaction centers at a given detergent concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411063
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  • 2
    Electronic Resource
    Electronic Resource
    Ruthenium red staining and tannic acid fixation of dental basement membrane (1981)
    Springer
    Cell & tissue research 220 (1981), S. 589-597 
    Details
    ISSN: 1432-0878
    Keywords: Tooth basement membrane ; Ruthenium red ; Tannic acid ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ruthenium red staining and tannic acid fixation were used to analyse the fine structure of embryonic mouse dental basement membrane in intact first mandibular molars or in EDTA-isolated dental papillae. Preameloblasts are separated from extracellular matrix proper by a basal lamina that contains regularly arranged proteoglycan granules of about 10 nm in diameter. This distribution pattern is particularly evident in the inner and outer lamina rara of the basal lamina associated with EDTA-isolated dental papillae. The plasmalemma of preameloblasts demonstrates electron dense plaques on the inner leaflet. Ruthenium red positive granules (50 nm in diameter) coat non-striated and striated fibrils of the matrix. Hyaluronidase treatment digested the ruthenium red positive granules. Tannic acid fixation allowed the demonstration of filaments within the lamina rara interna, connecting the lamina densa with plasmalemma of preameloblasts. These observations are discussed in the context of the terminal differentiation of odontoblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216762
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    Paper (German National Licenses)
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