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  • Life and Medical Sciences  (6)
  • Wiley-Blackwell  (6)
  • American Geophysical Union
  • Nature Publishing Group
  • Oxford University Press
  • Springer Nature
  • 1980-1984  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Fibronectin is apparently not involved in species-specific reaggregation of cells from the marine sponge geodia cydonium (1982)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 19 (1982), S. 395-404 
    Details
    ISSN: 0730-2312
    Keywords: fibronectin ; sponges ; Geodia cydonium ; aggregation ; cell recognition ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Experiments were carried out to test the hypothesis that fibronectin is involved in reaggregation of dissociated sponge cells. Cells from the siliceous sponge Geodia cydonium were extracted with urea to solubilize fibronectin from cells of higher multicellular organisms. The crude extract was further fractionated by DNA, heparin, and collagen affinity chromatography; they were termed Geodia fibronectinlike fractions. The fibronectinlike fractions contained a series of proteins with molecular weights different from that of the genuine fibronectin. The Geodia fibronectinlike fractions did not react with antiserum, produced against human fibronectin, under formation of a precipitin line. Using this antiserum the sponge cells could not be specifically labeled with FITC-anti-IgG antiserum. Radioimmunoprecipitation experiments revealed that the Geodia fractions contain - if at all - 0.1% fibronectin or fibronectinlike protein at the most. In the crucial experiments it was shown that the Geodia fibronectinlike fractions, human fibronectin, and antifibronectin antiserum exerted no influence on adhesion of Geodia cells either in the absence or in the presence of the soluble aggregation factor. Based on these findings, we conclude that fibronectin is apparently not present on Geodia cells and does not play a role in aggregation of this biological system.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240190408
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  • 2
    Electronic Resource
    Electronic Resource
    Proliferative characteristics of clonal endothelial cell strains (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 107 (1981), S. 123-137 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have utilized clonal strains of bovine fetal aortic endothelial cells to study cellular senescence in a differentiated cell type of physiological significance. Serial subcultivation of nine endothelial clones derived from three fetal calf aortas revealed proliferative life-spans in vitro of 53-125 population doublings (PDs), compared with 60 and 143 PDs for two lines of bovine fetal lung cells and 85 and 147 PDs for two lines of bovine vascular smooth muscle cells. Serial growth curves showed marked reductions associated with endothelial cellular senescence both in cellular growth rate and culture plateau density. Studies of the 24-hour [3H]-thymidine labeling index versus percentage of proliferative life-span completed indicated that clonal endothelial cultures contained a large proportion (greater than 90%) of rapidly cycling cells until about 75% of the life-spans were completed. Senescent endothelial cells showed evidence of large increases in cell area, cell volume, and protein content. In those clones examined, one specialized endothelial function, Factor VIII antigen expression, was retained qualitatively throughout the life-spans.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041070114
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  • 3
    Electronic Resource
    Electronic Resource
    Bovine endothelial cells transformed in vitro by benzo(a)pyrene (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 114 (1983), S. 328-338 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A cloned strain of bovine vascular endothelial cells with a finite in vitro lifespan was treated with benzo(a)pyrene (BP) after approximately 75% of its lifespan was completed. Untreated cultures of this strain senesced upon serial subcultivation and contained large, nondividing cells. In three out of seven trials, BP treatment produced transformed cells appeared in the cultures concomitant with the senescence of the parent cells. All transformed cell lines examined exhibited indefinite lifespans and altered karyotypes. Two of the lines retained most of the characteristics of normal endothelial cells, except that one became aneuploid and the other polyploid, Neither of these lines formed tumors when inoculated into nude mice. The remaining two lines retained mostly diploid kayotypes, but a high percentage of cells contained Robertsonian translocations. In one line cell volume was markedly reduced. In addition, these lines grew in multilayers, were anchorage independent, and proliferated in medium containing 0.5% serum. When 107 cells of these lines were injected into nude mice, tumors appeared within 1 week and were identified as malignant hemangioendotheliomas of bovine origin.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041140312
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  • 4
    Electronic Resource
    Electronic Resource
    Intra-acrosomally located acrosin-inhibitors: Evolution and developmental patterns in mammals (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 327-337 
    Details
    ISSN: 0148-7280
    Keywords: acrosin inhibitors ; acrosome ; evolution ; spermatogenesis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The acrosome of the mammalian spermatozoon contains proacrosin that is autocatalytically activated to become the proteolytic enzyme acrosin during the process of fertilization. Tschesche et al [1982] isolated specific acrosin inhibitors and suggested that they block prematurely activated acrosin. Antibodies against acrosin inhibitors purified from boar spermatozoa were used to demonstrate the evolutionaary relationship and the developmental pattern of the inhibitors in mammals. Using immunofluorescent techniques the following results were obtained: (1) The spermatozoa of man, boar, bull, ram, rat, rabbit, beaver, and mole stained positive in the acrosomal portion. (2) The round-headed spermatozoa of patients with globozoospermia and those in the ejaculates of fertile men lacked immunostaining for the inhibitors. (3) During spermatogenesis in all species, immunofluorescence for the acrosin inhibitors was first demonstrable in haploid spermatids and increased thereafter during spermatid differentiation. The stained area was found adjacent to the nuclear membrane, the position of the acrosome. (4) During teratogenesis of round-headed spermatozoa, the immunofluorescent staining for the inhibitors becomes separated from the nuclear membrane of the spermatids and is lost in late spermatids. Since identical results have been described for acrosin and acrosomal membrane proteins both in spermatozoa and spermatids of mammalian species and during spermiogenesis of patients with globozoospermia, our results are consistent with the localisation of the inhibitors in the acrosome. Immunostaining of spermatozoa of species belonging to five different mammalian orders with the antibody against boar acrosin-inhibitors is indicative of an evolutionary conservation of the inhibitors and their underlying genes.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100310
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  • 5
    Electronic Resource
    Electronic Resource
    Mikroskop (1982)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 12 (1982), S. 160-160 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19820120510
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  • 6
    Electronic Resource
    Electronic Resource
    Gene expression during gonadal differentiation in the rat: A two-dimensional gel electrophoresis investigation (1984)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 5 (1984), S. 27-42 
    Details
    ISSN: 0192-253X
    Keywords: gonad differentiation ; gene expression ; two-dimensional micro gel electrophoresis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Gonadal protein patterns were studied during development in the rat by two-dimensional micro-gel electrophoresis. Specific proteins were detected in both the male and the female sex at the morphologically indifferent state (two female- and one male-specific) and during differentiation. At the onset of gonadal differentiation (day 14) two additional sex-specific proteins were discovered in the male and two in the female. These proteins remained expressed during further development. One testicular protein was restricted to the cytosol of the tunica albuginea. The other one was absent from the tunica. In the female gonad, the two proteins were membrane-specific, one present in germ cells, the other in somatic cells. In the testis, one additional protein was discovered at postnatal day 1. Thus according to biochemical criteria there is no indifferent state of gonadal development. The testis and ovary express sex-specific genes both before and after the onset of gonadal differentiation.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020050103
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