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  • American Physical Society  (20)
  • Wiley-Blackwell  (17)
  • American Geophysical Union  (12)
  • 1980-1984  (49)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Mitochondrial movement during the ascidian sperm reaction (1983)
    New York, NY : Wiley-Blackwell
    Gamete Research 8 (1983), S. 295-307 
    Details
    ISSN: 0148-7280
    Keywords: sprem ; mitochondrion ; calcium ; calmodulin ; actin ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The ascidian sperm reaction, Which involves swelling, migration, and loss of the single large mitochondrion, can be triggered in vitro by raising the seawater pH to 9.3 or lowering Na+ to 20 mM, but only if the sperm are allowed to attach to a suitable Substate. Mitochondrial translocation does not usually occur in the absence of sperm attachment. Extracellular Ca2+ is necessary for triggering the reaction with low Na+ but not high pH; however, the intrecellular Ca2+ blocker, TMB-8, inhibits high pH-induced mitochondrial movement in the absence of extracellular Ca2+. After swelling, the mitochondrion fluoresces in the presence of chlortetracycline, suggesting that Ca2+ becomes membranebound after activation. Elevated cAMP and theophylline both inhibit mitochondrial move ment but not sperm motility. The antiactin drug cytochalasin B(10μM) and the calmodulinblocking drugs TFP (1 μM) and W-13 (10 μM) block mitochondrial movement, suggesting roles for actin and calmodulin in mitochondrial movement. A model is proposed relating intracellular alkalinization, Ca2+ influx, actin, myosin, and calmodulin in mitochondrial translocation.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120080309
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  • 2
    Electronic Resource
    Electronic Resource
    An outdoor biophotolytic system using the cyanobacterium Anabaena cylindrica B629 (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 213-220 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The cyanobacterium Anabaena cylindrica B629 was suspended in small glass beds and incubated in a gas-tight glass vessel outdoors under a gas atmosphere comprising carbon monoxide (0.2%), acetylene (5%), oxygen (6.5%), and nitrogen. The solution phase initially contained sodium bicarbonate (10mM) at pH 7. Under these conditions the organism continuously produced hydrogen gas for over three weeks. The temperature of the culture was maintained below 30°C and minimum night temperatures were recorded. The vessel was covered by a shadecloth, which reduced the natural illumination by approximately 70%. The system is an alternative to those requiring the strict absence of oxygen and little nitrogen, and requires virtually no attention during the incubation period.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260230114
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  • 3
    Electronic Resource
    Electronic Resource
    Simultaneous melting and freezing in the impingement region of a liquid jet (1980)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 26 (1980), S. 743-751 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An experimental investigation of an impinging water jet freezing on a melting solid surface has been carried out. Attention was focused on the stagnation region of an axisymmetric jet. In the experiment, a water jet was directed upward against the lower end of a meltable rod, having a diameter about twice that of the jet orifice. Solid octane (m.p. - 56.5°C) and solid mercury (m.p. - 38.9°C) served as the meltable materials. A laminar-axisymmetric flow model was developed to describe melting heat transfer in the presence of jet solidification within the impingement region. Measurements of the melting rate and conditions for the onset of jet solidification were found to agree quite well with the values predicted with this model.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690260507
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  • 4
    Electronic Resource
    Electronic Resource
    Experimental design in the clinical evaluation of amalgam restorations (1980)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 14 (1980), S. 777-788 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: In a clinical evaluation of marginal deterioration, 475 amalgram restorations were equally distributed in a 6 × 4 × 2 × 3 factorial design, based on alloy, operator, tooth, and number of restored surfaces. All factors except the last had a statistically significant effect after 6, 12 and 18 months. There were no consistent two-way interactions over time.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820140609
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  • 5
    Electronic Resource
    Electronic Resource
    Multistage carcinogenesis involves multiple genes and multiple mechanisms (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 121 (1984), S. 127-137 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041210416
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  • 6
    Electronic Resource
    Electronic Resource
    Temperature dependence of capacitation in bat sperm monitored by zona-free hamster ova (1981)
    New York, NY : Wiley-Blackwell
    Gamete Research 4 (1981), S. 525-533 
    Details
    ISSN: 0148-7280
    Keywords: capacitation ; fertilization ; spermatozoa ; in vitro ; Little Brown Bat ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The temperature dependence of capacitation in bat sperm (Myotis lucifugus lucifugus) was studied by monitoring fertilizations rates of zona-free hamster ova at different temperatures. Spermatozoa were cultured in BWW medium at temperatures 4°C, 24°C, 32°C, 42°C, and 55°C from 0-24 hr. Activation of sperm could be determined visually due to the change in movement seen through light microscopy. Activation was later confirmed by higher rates of fertilization. Preincubation of the bat sperm was found to have a direct effect on the success of penetration of the zona-free hamster ova. Holding bat spermatozoa at low temperature for long intervals allowed them to remain motile but unable to fertilize. Sperm are not irreversibly damaged, however, and activation, when the temperature is increased to 32°C, is faster than when sperm are intitially put at 32°C, resulting in good fertilization rates.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120040606
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  • 7
    Electronic Resource
    Electronic Resource
    Possible involvement of a sialylated component of the sperm plasma membrane in sperm-zona interaction in the mouse (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 153-163 
    Details
    ISSN: 0148-7280
    Keywords: mouse ; sperm ; egg ; sperm plasma membrane ; in vitro ; binding ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have studied the molecular mechanisms of gamete interaction in vitro in the laboratory mouse, Mus musculus. In particular, we were interested in whether this interaction is similar to a lectin-hapten-mediated process. Inhibition of sperm-zona binding was examined using various concentrations (.25 mM to 50 mM) of different sugars (sialic acid α-methylmannose, glucose, fucose, galactose, and N-acetyl-glucosamine). Sperm-zona binding was significantly decreased when eggs were pretreated with 10 mM of sialic acid or α-methylmannose but not by other sugars tested. Furthermore, treatment of capacitated sperm with neuraminidase destroyed their ability to bind and fertilize eggs. We have also used a specific lectin for sialic acid from the horseshoe crab (Limulus polyphemus) to agglutinate mouse sperm. The lectin (.120 ng/ml) mediated agglutination of mouse sperm (105 sperm/ml) was routinely observed to increase from a 10% agglutination immediately following their isolation from the epididymis to 100% agglutination 90 minutes later. Collectively, these results suggest the appearance of specific sugar moieties on the surface of the sperm plasma membrane which, in this particular species of mouse, are sialylated glycoproteins acting as ligands for specific receptors on the surface of the egg. These are the first data to indicate that sperm-egg recognition and attachment is a lectin-hapten-mediated process in the mouse.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100207
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  • 8
    Electronic Resource
    Electronic Resource
    Double inversion of the secondary nitrogens in cis-Diaziridinocyclopentane (1983)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 21 (1983), S. 706-710 
    Details
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 1α,2α,4α,6α,-3,7-Diazatricyclo[4.1.0.02,4]heptane (cis-diaziridinocyclopentane) (1) has been prepared from the analogous cis-diepoxycyclopentane. Ring opening of the diepoxide with sodium azide produced a pair of regioisomeric azido alcohols. Tosylation and treatment with lithium aluminum hydride produced 1. The dibenzoyl derivative possessed the di-exo stereochemistry for the tertiary aziridine nitrogens. The 1H spectrum of 1 was temperature dependent. Both the CH and NH resonances underwent decoalescence as the temperature was lowered. Because the rate was independent of concentration, the mechanism is probably inversion of the secondary nitrogen, the first such example to occur by the interchange of two diastereotopic, secondary (NH) amine nitrogens within the same molecule. The free energy of activation at coalescence (0 °C) was measured to be 12.8 kcal mol-1. The unsymmetrical slow exchange of 1 is clearly consistent with the exo,endo stereochemistry for the secondary aziridine nitrogens, possibly stabilized by intramolecular attraction.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/omr.1270211114
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  • 9
    Electronic Resource
    Electronic Resource
    The conformational energy difference for δ-valerolactone (1984)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 22 (1984), S. 613-615 
    Details
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The temperatuure dependence of the vicinal OCH2CH2 coupling constant in the six-membered ring δ-valerolactone suggests the presence of at least two conformers of unequal energy. Analysis of this temperature dependence by the Wood-Fickett-Kirkwood method in six solvents produces free energy differences in the narrow range 0.8-1.4 kcal mol-1, with a mean of 1.0 kcal mol-1 and no apparent dependence on solvent polarity. Thus the two conformers, probably the half-chair and the classic boat, have similar polarities. The analogous four- and five-membered lactones show little or no temperature dependence for their analogous coupling constants, in agreement with the presence of a single conformation for these rings.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1270221003
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  • 10
    Electronic Resource
    Electronic Resource
    Dependence of the γ carbon-13 shielding effect on the dihedral angle (1981)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 17 (1981), S. 265-269 
    Details
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The γ effects on 13C shielding have been examined for a series of rigid molecules containing the substituents CH3, OH, NH2, F, Cl, Br or I. Plots of the γ effect vs the C—C-α—C-β—X dihedral angle are well scattered if the plots do not distinguish modes of substitution. For the series in which the resonating carbon is CH2, however, fairly linear correlations are observed for X=CH3, OH, NH2, Cl and Br. It is concluded that within a structurally defined and substitutionally homogeneous series, there is a reasonably linear stereochemical dependence. Although substitution at C-α does not seriously alter the linear relationship, hybridization of the intermediate carbons may be important. The slopes of the plots for the γ effect vs dihedral angle are remarkably similar. Neither a strictly steric (van der Waals) nor a strictly polar source can be the cause of the linear relationship, since our series of substituents ranges widely with respect to both size and electronegativity. The only two factors that are constant within the series are the removal of the hydrogen atom (C—C—C—H to C—C—C—X) and the addition of the bonding and nonbonding orbitals on X that were not present on H.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1270170409
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