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  • Articles  (4)
  • oocyte  (2)
  • Alginate  (1)
  • Alnus  (1)
  • Springer  (4)
  • De Gruyter
  • 1980-1984  (4)
  • 1925-1929
Collection
  • Articles  (4)
Publisher
  • Springer  (4)
  • De Gruyter
Years
  • 1980-1984  (4)
  • 1925-1929
Year
  • 1
    ISSN: 1432-1424
    Keywords: Na−K ATPase ; ouabain ; Xenopus ; oocyte ; maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary During progesterone-stimulated maturation of defolliculated full-grownXenopus oocytes, the activities of the transport systems forl-alanine, thymidine, chloride, phosphate, and alkali ions decrease. Differences of the extent and time course of these changes suggest that they are controlled by at least partially independent mechanisms. A closer investigation of the Na-K ATPase has shown that in unstimulated oocytes, ouabain produces maximal inhibition when 8–12×109 molecules are bound per cell. This number is bound during the first phase of a diphasic uptake process. Since this phase can be suppressed by increasing the concentration of external K+ to 45 mmol/liter or more, it is concluded that it refers to binding to the Na−K pump in the plasma membrane. Ouabain bound prior to progesterone-induced germinal vesicle breakdown (GVBD) remains bound after the breakdown, although the Na−K pump loses the capacity to bind ouabain after GVBD in oocytes that had not been exposed to ouabain preceding GVBD. In the presence of Mg++ membranes isolated before regulatory inhibition of pumping and ouabain binding show a Na+-dependent incorporation of32P from γ-[32P]-ATP that can be reversed by the addition of K+. The phosphorylation site migrates on LiDS-polyacrylamide gel electropherograms at about 98,000 daltons and can be identified as a Commassie blue-stainable band.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1424
    Keywords: Xenopus ; oocyte ; cotransport ; alanine flux ; sodium flux ; sodium current
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The sodium-dependentl-alanine transport across the plasma membrane of oocytes ofXenopus laevis was studied by means of [14C]-l-alanine,22Na+ and electrophysiological measurements. At fixed sodium concentrations, the dependence of alanine transport on alanine concentration follows Michaelis-Menten kinetics; at fixed alanine concentrations, the transport varies with sodium concentration with a Hill coefficient of 2. In the presence of sodium the uptake of alanine is accompanied by a depolarization of the membrane. Under voltage-clamp conditions this depolarization can be compensated by an inward-directed current. Assuming that this current is carried by sodium we arrive at a 2∶1 stoichiometry for the sodium-alanine cotransport. The assumption was confirmed by direct measurements of both sodium and alanine fluxes at saturating concentrations of the two substrates, which also yielded a stoichiometry close to 2∶1. The sodium-l-alanine cotransport is neither inhibited by furosemide (0.5 mmol/liter) nor by N-methyl amino isobutyric acid (5 mmol/liter). A 20-fold excess ofd-alanine overl-alanine caused about 60% inhibition.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 65 (1982), S. 219-231 
    ISSN: 1573-5036
    Keywords: Alginate ; Carob gum ; France ; Inoculant ; Nodulation ; Polyacrylamide ; Polymer ; Rhizobium japonicum ; Senegal ; Soja ; Xanthan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field and cylinder experiments conducted in France and in Senegal showed that polyacrylamide, previously proposed as an entrapping gel for preparing Rhizobium inoculants, could be replaced by alginate (AER inoculant) or a mixture of xanthan and carob gum (XER inoculant). Semi-dried or dried AER and XER were used successfully provided that their storage time was less than 90 days. In soil inoculation trails, no marked differences were observed among semi-dried XER, dried AER, and dried XER. A number of seed inoculation experiments indicated that dried XER significantly outranked AER. Seeds preinoculated by up to 48 days with XER yielded plants which were comparable in nodulation and growth parameters to those derived from plant receiving peat inoculation at the time of planting.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 78 (1984), S. 235-243 
    ISSN: 1573-5036
    Keywords: Alnus ; Frankia ; N2-fixation ; Mixed stands ; Populus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Alders have an important role to play in biomass producing stands because of their N2-fixing ability and their capacity to withstand soils having an excess of moisture. The objectives of preliminary trials were (1) to find if there is any alder-genotype xFrankia-strain interaction when the effect of inoculating the bacteria was compared to no inoculation in seed beds of different species and provenances of alder, (2) to measure the possible effect of black alders interplanted in poplars compared to pure poplar plots. Two trials were laid out to study the alder-Frankia interaction. Both produced interaction. In the first one the inoculation had a favorable effect onAlnus glutinosa at age 2 years andA. cordata at age 1 and 2 and no effect onA. rubra. In the second one the inoculation had a depressive effect at age 1 on 2 of 3 provenances ofA. rubra and no effect on 1A. rubra, 3A.glutinosa and 3A. cordata provenances. A closely spaced field trial associating one black alder provenance and the poplar clone UNAL gives no superiority of mixed plots compared to pure plots. The results suggest that the N2-fixation of alders is not profitable to poplars at age 3 with a 1.5×2 m spacing.
    Type of Medium: Electronic Resource
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