Publication Date:
1980-11-21
Description:
A continuous hybrid cell line was derived that secretes monoclonal antibody capable of inhibiting the biological activity of mouse beta-nerve growth factor (beta-NGF). Results obtained with monovalent fragments indicate that the monoclonal antibody inhibits activity by interfering with the direct interaction between beta-NGF and the cell membrane receptor rather than by precipitating the dimeric form of beta-NGF. This monoclonal antibody binds to an antigenic determinant common to mouse beta-NGF, snake venom (Naja naja) beta-NGF, and human beta-NGF. These antibodies should provide specific molecular probes for a variety of studies of nerve growth factor including its tissue distribution and mechanism of action.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Warren, S L -- Fanger, M -- Neet, K E -- A110148/PHS HHS/ -- CA27915/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1980 Nov 21;210(4472):910-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6159686" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigen-Antibody Reactions
;
Clone Cells/immunology
;
Epitopes
;
Hybrid Cells
;
Immunoglobulin Fab Fragments
;
Male
;
Mice
;
Nerve Growth Factors/*antagonists & inhibitors/immunology
;
Receptors, Cell Surface/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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