ISSN:
1432-2048
Keywords:
Crassulacean acid metabolism induction
;
Kalanchoe
;
Phosphoenolpyruvate carboxylase (isoforms)
;
Photoperiodism
;
Sedum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Plants of Kalanchoe blossfeldiana v. Poelln. Tom Thumb and Sedum morganianum E. Walth. were grown under controlled photoperiodic conditions under either short or long days. Gaz exchange measurements confirmed that in K. blossfeldiana Crassulacean acid metabolism (CAM) was photoperiodically inducible and that S. morganianum performed CAM independently of photoperiod. With K. blossfeldiana, a comparison of catalytic and regulatory properties of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) from short-day and long-day grown plants showed differences, but not with S. morganianum. Ouchterlony double diffusion tests and immunotitration experiments (using a S. morganianum PEPC antibody) established that CAM is induced in K. blossfeldiana—but not in S. morganianum—through the synthesis of a new PEPC isoform; this form shows an immunological behavior different from that prevailing under non-inductive conditions and can be considered as specific for CAM performance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00393910
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