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  • Life and Medical Sciences  (5)
  • OCEANOGRAPHY  (5)
  • 1980-1984  (10)
  • 1950-1954
  • 1
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Journal of Morphology 163 (1980), S. 203-215 
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: As part of a study of ulcer formation and healing, regeneration of colonic mucosa in rats was studied following placement of a surgical lesion. Alterations in mucosubstances and connective tissue were examined and their possible significance discussed.The sequence of events in healing was: (1) The mucosa adjacent to the lesion tipped into the lesioned area. The crypts in this mucosa became lined with cells which contained no mucus and had no striated borders. Later in the experimental period, these undifferentiated cells gave rise to cells containing carboxymucins. Cells containing sulfomucin, neutral mucin, or having striated borders arose from the carboxymucin cells. (2) An epithelial ledge of undifferentiated cells migrated onto a sulfated glycosaminoglycan, fibrous interface between necrotic and living tissue in the lesion. (3) Crypt formation began with the appearance of intraepithelial anlagen. (4) Crypts lengthened by a process of epithelial-connective tissue proliferation from the base of the crypt upwards. Following completion of connective tissue regeneration, crypts formed by invading the reestablished lamina propria. (5) The first mucous cells in the ledge contained carboxymucins. As crypt formation occurred, these cells gave rise to typical columnar absorptive cells, to cells containing sulfomucins, and to cells containing neutral mucins. (6) Lengthening of crypts ceased following the appearance of a sulfated acid glycosaminoglycan - collagenous layer deep in the submucosa.
    Zusätzliches Material: 16 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 0730-2312
    Schlagwort(e): phosphomannosyl receptor ; pinocytosis ; fibroblast secretions ; glycopeptides ; acid hydrolases ; lysosomotropic amines ; β-hexosaminidase B ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: In a previous report we demonstrated that phosphorylated oligosaccharides isolated from acid hydrolases were subject to pinocytosis by phosphomannosyl receptors present on the cell surface of human fibroblasts [9]. However, limiting quantities of oligosaccharides precluded detailed comparison of the kinetics of pinocytosis of these phosphorylated oligosaccharides to those of the acid hydrolases from which they were derived. In this report we present studies comparing the kinetics of pinocytosis of acid hydrolases from NH4Cl-induced fibroblast secretions with those of concanavalin A-binding glycopeptides prepared from them by pronase digestion. The uptake of both secretion acid hydrolases and 125I-labeled glycopeptides was linear for at least 3 hr, saturable, inhibited competitively by mannose 6-phosphate, and destroyed by prior treatment of the ligand with alkaline phosphatase. The inhibition constants of excess unlabeled glycopeptide for the uptake of 125I-labeled glycopeptides (Ki of 1.5 × 10-6 M) and for the uptake of secretion acid hydrolases (Ki of 2.2 × 10-6 M) were remarkably similar. Furthermore, the Ki for mannose 6-phosphate inhibition of pinocytosis of glycopeptide uptake (3 × 10-5 M) compares closely to that previously determined for the pinocytosis of intact “high-uptake” acid hydrolases (3-6 × 10-5 M).“High-uptake” fractions of both ligands were prepared and quantified by affinity chromatography on immobilized phosphomannosyl receptors purified from bovine liver. Only 10% of the concanavalin A-binding glycopeptides bound to the immobilized phosphomannosyl receptors, while 80% of the acid hydrolases from which they were prepared bound and were eluted with 10 mM mannose 6-phosphate. However, the fraction of each type of ligand that binds to the immobilized phosphomannosyl receptors accounts for all the uptake activity of that ligand. The pinocytosis rates (% of added ligand internalized/mg protein/hr) of the “high-uptake” fraction of both intact acid hydrolase (12%/mg/hr) and glycopeptide (6%/mg/hr) differed by only twofold. The apparent Kuprake for both ligands was of the same order of magnitude. The similarity in the kinetics of pinocytosis of the secreted acid hydrolases and of the phosphomannose-bearing glycopeptides prepared from them suggests that the structural information which confers high-affinity binding to the phosphomannosyl receptor is contained in the glycopeptide units themselves. No additional information from the intact protein backbone appears essential for phosphomannosyl receptor-mediated pinocytosis.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Journal of Morphology 163 (1980), S. 191-201 
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Colonic organogenesis in rats was studied using light microscopic techniques for the demonstration of mucosubstances, glycogen, and connective tissue fibers.Crypts began as intraepithelial spaces which were in continuity with the colonic lumen. The cells forming the floors of these spaces invaded the nonsulfated acid glycosaminoglycan-rich mesenchyme as the basement membrane became discontinuous. As the diameter of the colon increased, the crypts lengthened and the lamina propria thickened until a layer of collagen and sulfated acid glycosaminoglycans formed at the bases of the crypts and the basement membrane was reestablished. The circular layer of the muscularis externa developed first, then the longitudinal layer, and finally the muscularis mucosae.Three types of mucous cells arose in these newly formed crypts. The initial epithelial cell type contained glycogen and gave rise to cells with apical coats of nonsulfated acid glycoproteins. This cell type was followed by the appearance of cells at the bases of the crypts containing nonsulfated acid glycoproteins. As the crypts lengthened, the goblet cells near the base contained nonsulfated and/or sulfated acid glycoproteins. Closer to and on the surface, the cells contained sulfated acid glycoproteins, a mixture of sulfated acid and neutral glycoproteins, or just neutral glycoproteins. Striated-border cells appeared intermingled with the mucous cells close to the bases of the crypts and continued onto the surface.A comparison was made between regeneration following placement of a surgical lesion in adult rats and events in organogenesis of the colon.
    Zusätzliches Material: 16 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 121 (1984), S. 284-290 
    ISSN: 0021-9541
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Influx of the K+ analogue Rb+ was measured through the ouabain-sensitive Na+/K+ pump and the ouabain-insensitive “leak” pathways in Cl- or NO-3 in mature red cells from adult pigs and in reticulocytes naturally occurring in 7-day-old piglets. In reticulocytes, Rb+ influxes by the two pathways were of about equal magnitude in Cl- (13 and 10 mmoles/liter cells × hr) and at least 25-fold larger than in mature red cells (0.5 and 0.4 mmoles/liter cells × hr). In Na + media, a portion of the ouabain-insensitive “leak” flux of Rb+ was Cl- dependent (Rb+Cl- transport) as NO-3 replacement reduced Rb+ influx by 90% in reticulocytes and by 40% in mature red cells. The sulfhydryl reagent N-ethylmaleimide (NEM) stimulated Rb+Cl- transport about twofold in reticulocytes and up to 13-fold in mature red cells. When reticulocytes matured to erythrocytes during in vitro incubation, about 90% of both ouabain-sensitive Rb+ pump and ouabain-insensitive Rb+Cl- influx were lost. In contrast, the NEM-stimulated Rb+Cl- transport changed much less throughout this period, suggesting an entity operationally but not necessarily structrually distinct from the basal Rb+Cl- transport. Although the experimental variability precluded a full assessment of significant changes in the small Na+/K+(Rb+) pump and Rb+Cl- fluxes in mature pig red cells kept for the same time period in vitro, Rb+ flux changes in reticulocytes appear to be maturational in nature, reflecting parallel activity transitions of Na+/K+ pump and Cl--dependent K+ fluxes in vivo.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 360-366 
    ISSN: 0021-9541
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Naturally occurring reticulocytes of week old piglets were used to characterize the maturation process under in vitro conditions. When the reticulocytes were suspended in tissue culture medium fortified with metabolic substrates, nearly all cells were viable after 24 hours incubation and usually more than 85% of the initial cell population survived after an 80 hour period. In cells maintained as long as a week in incubation, an adequate level of total adenine nucleotide with a large accumulation of IMP was found. In most cases, reticulocytes lose their reticular materials within two days and assume normal erythrocyte configuration. Concomitant with the morphological change, the cell volume decreases toward normal erythrocyte size, the extent of which can be accounted for by the intracellular loss of salt and accompanying water. As in the in vivo reticulocyte maturation process, reticulocytes undergoing in vitro maturation lose their membrane permeability to glucose. These findings suggest that the process of reticulocyte maturation occurring in cell culture approaches that which naturally occurs in vivo. Thus, these cells may be used to delineate the mechanism of the loss of membrane transport of glucose which normally occurs in the adult pig cells.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Publikationsdatum: 2011-08-17
    Beschreibung: Chlorophyll gradient maps of large ocean areas were generated from U-2/OCS data obtained over test sites in the Pacific and the Atlantic Oceans. The delineation of oceanic features using the upward radiant intensity relies on an analysis method which presupposes that radiation backscattered from the atmosphere and the ocean surface can be properly modeled by using a measurement made at 778 nm. The calculation of atmospheric radiance was performed by using a method developed by Dave. An estimation of the chlorophyll concentration is performed by properly ratioing radiances measured at 472 and 548 nm after removing the atmospheric effects. The correlation between the remotely sensed data and the in situ surface chlorophyll measurements has been validated in two sets of data. The results show that the correlation between the in situ measured chlorophyll and the derived quantity is a negative exponential function, and the correlation coefficient was calculated to be -0.965.
    Schlagwort(e): OCEANOGRAPHY
    Materialart: Journal of Geophysical Research; 85; July 20
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Publikationsdatum: 2011-08-18
    Beschreibung: The Ocean Color Experiment was one of the six remote-sensing experiments which for the first time were launched and successfully operated on board of the second flight of the Space Shuttle during November 1981. The experiment consists of a multispectral image scanner dedicated to the measurement of water color and its interpretation in terms of major water constituents and circulation patterns. The objectives of the experiment, the test site selection, and associated activities are described. The actual mission and results of an initial data analysis is discussed.
    Schlagwort(e): OCEANOGRAPHY
    Materialart: (ISSN 0196-2892)
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    facet.materialart.
    Unbekannt
    In:  Other Sources
    Publikationsdatum: 2011-08-18
    Beschreibung: NASA's Ocean Color Experiment (OCE) was designed to map ocean features with an eight-channel scanning radiometer installed on the second flight of the Space Shuttle Columbia. Digital computer enhancement and band-ratio techniques were applied to radiometrically corrected spectral data in order to emphasize chlorophyll distribution patterns. Such a pattern was evident in the Yellow Sea between Korea and China, and the effects of discharge from the Yangtze and other rivers were also observed. Two scenes, from orbits 30 and 32, revealed the movement of plankton patches in the Gulf of Cadiz. Geometric correction of the images permitted ocean current velocities to be deduced, and water depth variability over the Grand Bahama Bank was estimated by means of OCE's blue-green channel. Bottom-reflected sunlight produced a sensor signal that was inversely related to water depth.
    Schlagwort(e): OCEANOGRAPHY
    Materialart: Science; 218; Dec. 3
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Publikationsdatum: 2019-06-28
    Beschreibung: An ocean atmosphere radiative transfer process computation method which is suitable for determining lower boundary ocean albedo and other radiation components from spectral measurements of upwelling radiance taken from a high altitude platform is described. The method was applied to a set of color scanner data taken from slope water of the South Atlantic Bight to determine the influence of cholorophyll-a pigments in the sea on the ratio of upwelling radiance to down welling irradiance as a function of wavelength. The resulting chlorophyll concentrations are compared with measurements made by ships stationed along the flight path.
    Schlagwort(e): OCEANOGRAPHY
    Materialart: NASA-TM-84961 , NAS 1.15:84961
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    facet.materialart.
    Unbekannt
    In:  Other Sources
    Publikationsdatum: 2019-07-13
    Beschreibung: Ocean Color Experiment (OCE) on the OSTA-1 mission acquired ocean images at several widely separated locations on the earth. Digital computer enhancement and band ratioing techniques were used to emphasize patterns of chlorophyll and sediment distribution in the Yellow Sea and, in one case, of bottom topography in the Great Bahama Bank. Two scenes in the Gulf of Cadiz from orbits 30 and 32 were geometrically corrected to show the movement of plankton patches. This technique enabled ocean current velocities to be deduced. A duplicate of the OCE instrument mounted on a DFVLR aircraft was flown over an area of the Atlantic Ocean off the coast of Portugal during the Shuttle mission.
    Schlagwort(e): OCEANOGRAPHY
    Materialart: AIAA PAPER 83-0415 , American Institute of Aeronautics and Astronautics, Aerospace Sciences Meeting; Jan 10, 1983 - Jan 13, 1983; Reno, NV
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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