ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
It is demonstrated that photoacoustic spectroscopy is a superior tool in the evaluation of electropherograms. The locla distribution of proteins in ultrathin layers of polyacrylamide gels used for isoelectric focusing is mapped with a resolution equal to or better than a laster densitomer. Even unstained samples of colored proteins can be mapped with excellent sensitivity and resolution due to the fact that the photoacoustic effect is caused only by the absorbed fraction of the incident light; scapttered light therefore doesse not cause a signal. in situ photoacosutic spectra of previously localzed zones allow the identification of the prosthetic groups of these proteins.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150040209
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