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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of monovalent sexual binding components from Chlamydomonas eugametos flagellar membranes (1980)
    Springer
    Archives of microbiology 128 (1980), S. 120-125 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Sex ; Isoagglutination ; Membrane vesicles ; Glycoproteins ; Cell interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several treatments were tested to extract the sexual binding site from membrane vesicles derived from the flagellar surface of Chlamydomonas eugametos. Extraction with detergents, chaotropic and hydrogen bond-disrupting agents, as well as sonication, was effective in reducing the isoagglutination activity of these membrane vesicles. Complementary with this reduction, a sex-specific biological activity related to isoagglutination, called “twitch activity” appeared in the extract. This was only observed with vesicles derived from minus mating type (mt-) gametes. After fractionation of the extract, one high-molecular weight glycoprotein fraction appeared to be responsible for this activity. When extracts were treated with cross-linking agents, a pelletable fraction was obtained with isoagglutinative activity. We conclude that the mt- factor, responsible for twitch activity, causes isoagglutination when it is rendered multivalent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422315
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  • 2
    Electronic Resource
    Electronic Resource
    Sex-specific glycoproteins in Chlamydomonas flagella an immunological study (1980)
    Springer
    Archives of microbiology 126 (1980), S. 77-81 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Sex ; Flagella ; Agglutination ; Glycoproteins ; Immunochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To identify mating type-specific glycoproteins associated with the flagellar membrane of Chlamydomonas eugametos, which could be involved in sexual agglutination, antibodies were raised in rabbits against purified gamete flagella of either mating type. The immunoglobulin (Ig) fractions exhibited partial mating-type specificity in agglutinating gametes, in the indirect immunofluorescence test and in the crossed immunoelectrophoresis test. This specificity was strongly enhanced by absorbing the fractions with flagella of the opposite mating type. Absorbed Ig fractions produced a single precipitation line with Triton extracts of gamete flagella in the crossed immunoelectrophoresis technique. On polyacrylamide gel electrophoresis this line appeared to contain two flagellar glycoprotein fractions, PAS 1 and PAS 4. Polyacrylamide gels of flagellar extracts incubated with these Ig fractions, followed by staining with peroxidase-anti-rabbit Ig resulted in the staining of only the PAS 1 and PAS 4 bands, which confirms that these components of the flagellar membrane are mating type-specific antigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421894
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    Paper (German National Licenses)
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