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  • Springer  (315)
  • 1980-1984  (241)
  • 1965-1969  (74)
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  • 1
    ISSN: 1432-2048
    Keywords: Chlorophyll fluorescence ; Chloroplast development ; Thylakoid protein kinase ; Triticum (chloroplast biogenesis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The activity of thylakoid protein kinase and the regulation of excitation energy distribution between photosystems I and II was examined during chloroplast biogenesis in light-grown Triticum aestivum (wheat) leaves. The specific activity of the thylakoid protein kinase decreased some six-fold during development from the young plastids at the base of the 7-d-old leaf to the mature chloroplasts at the leaf tip. Appreciable activity was also detected in plastids isolated from etiolated leaves. In mature chloroplasts the majority of phosphate was incorporated into the Mr=26,000 apo-proteins of the light-harvesting chlorophyll a/b-protein complex (LHCP). However, at early stages of chloroplast development and in the etioplast, the phosphate was predominantly incorporated into a polypeptide of Mr=9,000 dalton. Immature thylakoids, isolated from the base of the leaf, had relatively low concentrations of LHCP and could perform a State 1-State 2 transition, as demonstrated by ATP-induced quenching of photosystem II fluorescence. Analyses of photosystem I and photosystem II fluorescence-induction curves from intact leaf tissue demonstrated that this transition occurs in vivo at early stages of leaf development and, therefore, may play an important role in regulating energy transduction during chloroplast biogenesis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-072X
    Keywords: Poteriochromonas ; Euglena ; Tetrahymena ; Crithidia ; Vitamin excretion ; Vitamin B12 ; Biotin ; Thiamin ; Folates ; Vitamin B6 ; Riboflavin ; Nicotinic acid ; Pantothenate ; Vitamin C ; Bioterin ; Phytoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Vitamins B6 and B12, biotin, folates, riboflavin, nicotinate, pantothenate, biopterin, and vitamin C (l-ascorbate) were assayed in Poteriochromonas stipitata, Euglena gracilis, and Tetrahymena thermophila cells grown in defined media and in spent culture supernatants. P. stipitata and E. gracilis synthesized, stored and excreted folates (mainly as 5-methyltetrahydrofolate), B6, riboflavin, pantothenate, nicotinate, biopterin, and ascorbate. E. gracilis synthesized and stored biotin. T. thermophila did not synthesize the above vitamins except for B12, biopterin, and ascorbate; it excreted biopterin and stored B12 and ascorbate. Thiamin was left of consideration because all 3 organisms are thiamin auxotrophs. Possible ecological implications of these findings are considered.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 83 (1981), S. 295-301 
    ISSN: 1573-5117
    Keywords: seasonal ; phytoplankton ; Mississippi River
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Species composition and seasonal succession of the phytoplankton were investigated on the upper Mississippi River at Prairie Island, Minnesota, U.S.A. Both the numbers and volume of individual species were enumerated based on cell counts with an inverted microscope. A succession similar to algal succession in the local lakes occurred. The diatoms were dominant during the spring and fall and blue-green algae were dominant during the summer. The algal concentrations have increased up to 40 fold the concentrations of the 1920's, since the installation of locks and dams. The maximum freshweight standing crop was 4 mg · l−1 in 1928 (Reinhard 1931), 13 mg · l−1 in 1975 a wet year, and 47 mg · l−1 in 1976, a relatively dry year with minimal current discharge. The diatoms varied from 36–99%, the blue-green algae from 0–44% and the cryptómonads from 0–50% of the total standing crop. The green algae were always present but never above 21% of the biomass. The dominant diatoms in recent years were centric -Stephanodiscus andCyclotella spp. (maximum 50,000 ml−1). The dominant blue-green algae wereAphanizomenon flos-aquae (L.) Ralfsex Born.et Flahault andOscillatoria agardhii Gomont (maximum 800 ml−1). These algal species are also present in local lakes. Shannon diversity values indicated greatest diversity of algae during the summer months.
    Type of Medium: Electronic Resource
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  • 4
  • 5
    Publication Date: 1981-09-01
    Print ISSN: 0018-8158
    Electronic ISSN: 1573-5117
    Topics: Biology
    Published by Springer
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  • 6
    Publication Date: 1983-10-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Mathematische Zeitschrift 104 (1968), S. 231-246 
    ISSN: 1432-1823
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Mathematische Zeitschrift 104 (1968), S. 252-256 
    ISSN: 1432-1823
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Mathematische Zeitschrift 178 (1981), S. 509-525 
    ISSN: 1432-1823
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 68 (1982), S. 107-140 
    ISSN: 1432-1424
    Keywords: exocytosis ; calcium ; adrenal medulla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary By subjecting isolated adrenal medullary cells to intense electric fields of brief duration it is possible to gain access to the cell interior without impairing the ability of the cell to undergo exocytosis. After a single exposure to a field of 2 kV/cm, τ=200 μsec, adrenal medullary cells behave as if their plasma membrane contains two pores of effective radius 2 nm. At 37°C these ‘equivalent pores’ remain patent for up to 1 hr. The formation and stability of these ‘pores’ is not affected by the Ca content of the bathing solution. The ‘pores’ permit externally applied catecholamine and Ca-EGTA to equilibrate rapidly with the cell water. Cells rendered ‘leaky’ in K glutamate medium containing 5mm Mg-ATP and EGTA to give an ionized Ca close to 10−8 m release less than 1% of their total catecholamine. These same cells can release up to 30% of their catecholamine when exposed to 10−5 m Ca. This Ca-dependent release is unaffected by Ca-channel blockers such as D600. Catecholamine release in response to a calcium challenge only seems to occur during the first few minutes whilst the Ca concentration is changing, and the extent of release depends on the final Ca concentration achieved. Half-maximal release occurs at about 1 μm Ca, and this value is independent of the EGTA concentration used to buffer the ionized Ca. The relation between ionized Ca and catecholamine release is best fitted by a requirement for 2 Ca ions. Calcium-evoked release of catecholamine is associated with the release of dopamine-β-hydroxylase (DβH) but not lactate dehydrogenase. The ratio DβH/catecholamine released is the same as that in stimulated intact cells and perfused glands. The time course of appearance in the external medium of DβH and catecholamine is identical. Transmission electron microscopy of ‘leaky’ cells exposed to 10−8 m Ca reveals no marked differences from unstimulated intact cells. The cytoplasm of ‘leaky’ cells exposed to 10−5 m Ca contains large membrane-bounded vacuoles. When secretion is caused to take place in the presence of horseradish peroxidase, this marker is found within the vacuoles. Ca-dependent release of both catecholamine and DβH requires Mg-ATP. Cells equilibrated with Ca in the absence of Mg-ATP can be triggered to undergo exocytosis by the addition of Mg-ATP. In the absence of Mg, ATP alone is ineffective. Of a variety of other nucleotides tested, none is as effective as ATP. Mg-ATP affects the extent of exocytosis and not its apparent affinity for Ca. Replacement of glutamate as the major anion by chloride results in a marked reduction in Ca-dependent release of both catecholamine and DβH. Chloride causes a small increase in Ca-independent release of catecholamine, a large reduction in the extent of exocytosis, and a decrease in the apparent affinity of exocytosis for Ca. Of a variety of anions examined, their order of effectiveness at supporting Ca-dependent exocytosis is glutamate−〉acetate−〉Cl−〉Br−〉SCN−. Exocytosis is not obviously affected by replacing K by Na or sucrose or by altering the pH over the range pH 6.6 to 7.8. Raising the free Mg concentration reduces the extent of Ca-dependent exocytosis and also its apparent affinity for calcium. Calcium-dependent exocytosis in ‘leaky’ cells is largely unaffected by (i) a variety of agonists and antagonists of the nicotinic receptor; (ii) agents that disrupt microtubules and microfilaments; (iii) phalloidin; (iv) vanadate; (v) inhibitors of anion permeability; (vi) protease inhibitors; and (vii) agents that dissipate the vesicle pH gradient and potential. It is partially inhibited by (i) certain antipsychotic drugs; (ii) a rise in osmotic pressure, (iii) lowering the temperature below 20°C, and (iv) N-ethyl maleimide.
    Type of Medium: Electronic Resource
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