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  • Articles  (128)
  • Chemistry  (125)
  • Glucose  (3)
  • 42.75
  • Atomic and molecular processes in external fields, including interactions with strong fields and short pulses
  • 1980-1984  (46)
  • 1970-1974  (82)
Collection
  • Articles  (128)
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Year
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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of the synthesis of methanol oxidizing enzymes inKloeckera sp. 2201 andHansenula polymorpha, a comparison (1982)
    Springer
    Archives of microbiology 131 (1982), S. 174-175 
    Details
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Kloeckera sp. 2201 ; Methanol ; Glucose ; Methanol dissimilating enzymes ; Regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparative study was made of the regulation of the synthesis of methanol dissimilating enzymes inkloeckera sp. 2201 andHansenula polymorpha using chemostat and batch growth conditions and methanol or glucose as carbon sources. During growth in methanol-limited chemostat cultures similar enzyme patterns for alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase in the two yeasts were found. When growing in batch culture with glucoseH. polymorpha, but notKloeckera sp. 2201, was found to produce ethanol which might affect the synthesis of these enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01054002
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  • 2
    Electronic Resource
    Electronic Resource
    Mixed substrate growth of methylotrophic yeasts in chemostat culture: Influence of the dilution rate on the utilisation of a mixture of glucose and methanol (1982)
    Springer
    Archives of microbiology 131 (1982), S. 8-13 
    Details
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Kloeckera sp. 2201 ; Mixed substrate utilisation ; Chemostat ; Induction ; Repression ; Methanol ; Glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of Hansenula polymorpha and Kloeckera sp. 2201 with a mixture of glucose and methanol (38.8%/61.2%, w/w) and the regulation of the methanol dissimilating enzymes alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase were studied in chemostat culture, as a function of the dilution rate. Both organisms utilized and assimilated glucose and methanol simultaneously up to dilution rates of 0.30 h-1 (H. polymorpha) and 0.26h-1, respectively (Kloeckera sp. 2201) which significantly exceeded μmax found for the two yeasts with methanol as the only source of carbon. At higher dilution rates methanol utilisation ceased and only glucose was assimilated. Over the whole range of mixed-substrate growth both carbon sources were assimilated with the same efficiency as during growth with glucose or methanol alone. In cultures of H. polymorpha, however, the growth yield for glucose was lowered by the unmetabolized methanol at high dilution rates. During growth on both carbon sources the repression of the synthesis of all catabolic methanol enzymes which is normally caused by glucose was overcome by the inductive effect of the simultaneously fed methanol. In both organisms the synthesis of alcohol oxidase was found to be regulated differently as compared to catalase, formaldehyde and formate dehydrogenase. Whereas increasing repression of the synthesis of alcohol oxidase was found with increasing dilution rates as indicated by gradually decreasing specific activities of this enzyme in cell-free extracts, the specific activities of this enzyme in cell-free extracts, the specific activities of catalase and the dehydrogenases increased with increasing growth rates until repression started. The results indicate similar patterns of the regulation of the synthesis of methanol dissimilating enzymes in different methylotrophic yeasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00451491
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  • 3
    Electronic Resource
    Electronic Resource
    Regulatory flexibility of methylotrophic yeasts in chemostat cultures: Simultaneous assimilation of glucose and methanol at a fixed dilution rate (1982)
    Springer
    Archives of microbiology 131 (1982), S. 1-7 
    Details
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Kloeckera sp. 2201 ; Chemostat ; Mixed substrates ; Glucose ; Methanol ; Growth yields ; Enzyme regulation ; Dissimilatory enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of the composition of methanol/glucose-mixtures as only sources of carbon and energy on growth and regulation of the synthesis of enzymes involved in methanol-dissimilation was studied under chemostat conditions at a fixed dilution rate with the methylotrophic yeasts Hansenula polymorpha and Kloeckera sp. 2201. Both carbon sources were found to be utilized completely independently of the composition of the C1/C6 mixture. Using mixtures of 14C-labelled methanol and glucose the growth yield for glucose was found to be constant for all C1/C6-mixtures tested and both yeasts. The growth yield for methanol, however, was reduced by up to 25% when the proportion of methanol in the inflowing medium was lower than 20% (w/w with respect to glucose) for H. polymorpha and 50% (w/w with respect to glucose) for Kloeckera sp. 2201 respectively. During growth with C1/C6-mixtures containing higher C1-proportions of methanol regular growth yields for methanol were recorded which corresponded to the growth yields found with methanol as the only carbon source. The regulation of the synthesis of the enzymes of the dissimilatory pathway for methanol was found to be under multiple control. Although glucose was present in the medium methanol had a positive effect on the synthesis of these enzymes. Thus, in addition to derepression induction by methanol was also observed. This inductive effect was found to increase with increasing proportions of methanol in the mixture. Depending on the enzyme, 10–40% methanol in the mixture resulted in a maximal induction with enzyme specific activities equal to those found in cells grown with methanol as the only carbon source. No further enhancements in enzyme specific activities were observed during growth on mixtures containing more than 40% methanol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00451490
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  • 4
    Electronic Resource
    Electronic Resource
    An electron microscopic method for studying nucleic acid-protein complexes. Visualization of RNA polymerase bound to the DNA of bacteriophages T7 and T3Dedicated to the memory of Dr. Jean-Pierre Tranzer. (1974)
    New York : Wiley-Blackwell
    Biopolymers 13 (1974), S. 995-1009 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Double-stranded DNA can be readily adsorbed on mica or directly on carbon coated grids from the surface of solutions containing ethidium bromide, actinomine, or propidium diiodide. The DNA molecules are unfolded, well separated, and show a length distribution similar to molecules prepared by protein monolayer techniques.Since the intercalating dyes tested do not lead to an increased apparent diameter of the nucleic acid the method is useful for the study of nucleic acid-protein complexes. As a model, the binding of E. coli RNA polymerase to phage T7 and T3 DNA was examined under different conditions. The enzyme can easily be identified and its position along the DNA molecule can be mapped.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1974.360130514
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  • 5
    Electronic Resource
    Electronic Resource
    Zellphysiologie des Stoffwechsels. Von D. Pette. Bd. 13 der Konstanzer Universitätsreden. Herausgeg. von G. Hess. Konstanzer Universitätsverlag 1970. 50 S., brosch. DM 4,80 (1970)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 4 (1970), S. 196-196 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19700040613
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  • 6
    Electronic Resource
    Electronic Resource
    Wasser. Lösungsmittel, Lebensraum und Ökofaktor. Von R. Wittig. Akademische Verlagsgesellschaft, Wiesbaden 1979. VIII, 184 S., Zeichn. u. Tab., kart. DM 24, - (1980)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 14 (1980), S. 103-103 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19800140306
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  • 7
    Electronic Resource
    Electronic Resource
    Physikalische Methoden in der Chemie: UV-Photoelektronenspektroskopie (1971)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 5 (1971), S. 65-77 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 19 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19710050302
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  • 8
    Electronic Resource
    Electronic Resource
    Notiz über ein einfaches Verfahren zur Darstellung von 3,4-Bismethylencyclobuten (1973)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 106 (1973), S. 3461-3462 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19731061031
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  • 9
    Electronic Resource
    Electronic Resource
    [2.2]Paracyclophane durch Addition von Acetylenderivaten an 1,2,4,5-Hexatetraen (1974)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 107 (1974), S. 1891-1902 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Preparation of [2.2]Paracyclophanes by Addition of Acetylene Derivatives to 1,2,4,5-HexatetraeneThe tetrasubstituted [2.2]paracyclophanes 4a - e are formed by cycloaddition of the symmetrical acetylene derivatives 2a - e to 1,2,4,5-hexatetraene (1), and their constitution and configuration is established by spectroscopic methods, degradation and pyrolysis experiments. The tetramethyl ester 4a is reduced to the tetraalcohol 4g, and the tetracarboxylic acid 4c is converted to the bisanhydride 4f and [2.2] paracyclophanes (4h), respectively. All possible disubstituted [2.2] paracyclophanes 6 are obtained when methyl propiolate (5a) is added to. 1, whereas further symmetrical and unsymmetrical acetylene derivatives do not react with 1.
    Notes: Die tetrasubstituierten [2.2]Paracyclophane 4a - e werden durch Cycloaddition der symmetrischen Acetylenderivate 2a - e an 1,2,4,5-Hexatetraen (1) dargestellt und ihre Konstitution und Konfiguration durch spektroskopische Methoden. Abbau- und Pyrolyseversuche bewiesen. Der Tetramethylester 4a wird zum Tetraalkohol 4g reduziert und die Tetracarbonsäure 4c in das Bisanhydrid 4f bzw. [2.2] Paracyclophan (4h) übergeführt. Bei der Anlagerung von Propiolsäure-methylester (5a) an 1 werden alle isomeren disubstituierten [2.2]paracyclophanes 6 erhalten. Weitere symmetrische und unsymmetrische Acetylenderivate reagieren nicht mit 1.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19741070613
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  • 10
    Electronic Resource
    Electronic Resource
    Die Genehmigungsverfahren nach dem Bundes-Immissionsschutzgesetz. Von M. Pütz und K. H. Buchholz. Erich Schmidt Verlag. Berlin-Bielefeld-München 1982. 1. Aufl., 445 S., zahlr. Abb. und Tab., geb., DM 128,- (1983)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 55 (1983), S. 515-515 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330550704
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