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  • Senescence  (2)
  • Thermofraktographie  (2)
  • 1980-1984  (2)
  • 1970-1974  (2)
  • 1955-1959
  • 1950-1954
  • 1945-1949
  • 1
    Electronic Resource
    Electronic Resource
    Transformation to senescence with plasmid like DNA in the ascomycete Podospora anserina (1980)
    Springer
    Current genetics 2 (1980), S. 181-184 
    Details
    ISSN: 1432-0983
    Keywords: Transformation ; Senescence ; Podospora anserina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the ascomycete Podospora anserina senescence through strain aging is under nucleo-cytoplasmic control and inducible in juvenile mycelia by an ‘infective principle’ transferred after cytoplasmic contact via anastomoses. A specific DNA called plasmid-like (pl) DNA, present exclusively in aging mycelia, was found to be identical with this ‘infective principle’, since it was possible to transform juvenile protoplasts to senescence by using purified p1DNA. Therefore a specific function may be attributed to this ccc DNA. Its direct involvement in a genetically programed senescence is confirmed and its development as a vector for transfer of genetic information in eukaryotes can be undertaken.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435683
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  • 2
    Electronic Resource
    Electronic Resource
    Development of a eukaryotic cloning system in Podospora anserina (1982)
    Springer
    Current genetics 6 (1982), S. 219-222 
    Details
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Eukaryotic cloning system ; Senescence ; Long-lived mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In developing a system for molecular cloning with the Podospora anserina plasmid (p1DNA) it is necessary to find recipient strains which are resistant to p1DNA mediated senescence. Three long lived double mutants which fail to exhibit spontaneous aging were genetically and biochemically analysed. All mutants were infected with p1DNA. The mutant ca viv became irreversibly senescent and therefore was not further tested. The second mutant, gr viv showed some symptoms of aging but never died. The third strain i viv remained resistant to aging from p1DNA infection and has thus proven to be the best host strain available for molecular cloning in this system. A DNA analysis of the latter two strains revealed: 1. There is no difference from the wild strain with respect to the structure of mtDNA and the integration site of the p1DNA. 2. Of the two strains, only i viv contains free p1DNA in its mitochondria but in low amounts if compared to the wild strain. These experimental results are interpreted as follows: 1. The gr viv strain does not liberate spontaneously the p1DNA from mtDNA, but following infection is able to replicate and express this plasmid and therefore is a potential host for transformation. 2. The i viv strain liberates the mitochondrial plasmid but does not express senescence even when infected with p1DNA. Therefore, this strain is an ideal recipient for transformation provided a marker other than senescence is cloned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390341
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  • 3
    Electronic Resource
    Electronic Resource
    Thermofraktographie (1972)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 261 (1972), S. 11-21 
    Details
    ISSN: 1618-2650
    Keywords: Thermofraktographie ; Apparatur und Analysenbeispiele. Chromatographie, Dünnschicht ; Kopplungsverfahren. Thermoextraktion ; flüchtige Substanzen, Ultramikrobereich. TAS-Verfahren ; thermisches Mikro-AbtrennTransfer-Auftrageverfahren. Thermolyse ; Pyrolyse, Polymermaterial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Weiterentwicklung des thermischen Mikro-Abtrenn-Transfer- und Auftrageverfahrens (TAS) führte zur Thermofraktographie (TFG). Die hierzu notwendigen apparativen Vorrichtungen (TASOMAT und Steuerteil) sind beschrieben. Wenige Milligramme einer Probe werden bei der TFG im linearen Temperaturanstieg z.B. von 20 auf 450° C erhitzt. Die flüchtigen und kondensierbaren Bestandteile, sowie die Thermolyseprodukte von Polymermaterial fängt man dabei kontinuierlich und fraktioniert als Startband auf einer DC-Platte auf. Nach der Chromatographie erhält man auf diesem Wege auch aus komplexen Proben bislang schwer analysierbarer Gemische, wie z.B. Kaugummi, Lignine, gefärbte Mischgewebe, Fette mit Zusätzen usw., schnelle Informationen über die Zusammensetzung.
    Notes: Abstract The further development of the Thermo-Micro-Extraction, Transfer and Application Procedure (TAS) resulted in Thermofractography (TFG). The necessary apparatus for this (TASOMAT and regulating mechanisms) is desribed. In TFG a few milligrams of a sample are heated with linear rise of temperature from, e.g., 20° C–450° C. The volatile and condensable components, as well as the products of thermolysis of polymeric material, are caught continually and fractioned as the starting line of a TLC-plate. After chromatography, rapid information can thus be obtained about the components of a complex sample of mixtures which were hithertoo difficult to analyse, like e.g., chewing gum, lignins, coloured mixed textiles and fats with additives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00430782
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  • 4
    Electronic Resource
    Electronic Resource
    Thermofraktographie zur Charakterisierung natürlicher Polyphenole, Gerbstoffdrogen und Leder (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1973), S. 81-92 
    Details
    ISSN: 1618-2650
    Keywords: Charakterisierung von Phenolen ; Polyphenolen ; Gerbstoffen ; Gallotanninen ; Catechinen ; Leder ; Chromatographie ; Dünnschicht ; Thermofraktographie ; Thermolyse ; Pyrolyse ; Polyphenole ; Gerbstoffe ; Leder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Unter den Bedingungen der Thermofraktographie (TFG) wurde das Verhalten einfacher Phenole, definierter Gerbstoffe und deren Bausteine (Modellsubstanzen) sowie von Gerbstoffdrogen und Ledern im linearen Temperaturanstieg von 50–450° C untersucht. Zuvor mußte die DC der zu erwartenden Phenole optimiert werden. Die Thermofraktogramme (TFG) der Modellsubstanzen zeigten die verschiedenartige Fragmentierung je nach ihren Bausteinen und deren Verknüpfung. Anhand der Thermolysebereiche und der auftretenden Phenole im TFG ist nun die Zuordnung zu einer der Gerbstoffklassen möglich geworden. Dies gelingt auch, wenn die Gerbstoffe, wie im Leder, in fester H-Brückenbindung an Kollagen vorliegen. Zusätzlich erhält man Hinweise auf die bei der Zurichtung und Färbung des Leders verwendeten Stoffe. F:ur einige wichtige natürliche und synthetische Gerbstoffe sind die TFG abgebildet und besprochen.
    Notes: Abstract In this report the behaviour of a number of phenolic substances of natural and synthetic origin-e.g. monomeric phenols, defined tanning agents and their constitutional units (model substances), tanning drugs and leather-was investigated under the conditions of thermofractography (TFG), with linear increase of temperature in the range 50–450° C. The TLC conditions had to be optimized first in order to achieve the best possible identification of the phenols expected to originate during TFG. The TFG of the model substances showed varying fragmentations according to their constitutional units and their types of linkage. Regarding the thermal range of fragmentation and the phenols which appear in the thermofractograms (TFG), the assignment to the types of tanning agents in plant material has become possible. The same assignment can be made for the tanning agents which are bound in collagen (leather) by hydrogen bonds. Further, TFG gives hints about substances which have been used for finishing and dyeing leather. The figures of the thermofractograms of some natural and synthetic tanning agents are given and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423959
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