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  • 1
    Electronic Resource
    Electronic Resource
    Membrane glycoproteins of Chlamydomonas eugametos flagella (1979)
    Springer
    Planta 145 (1979), S. 417-425 
    Details
    ISSN: 1432-2048
    Keywords: Chlamydomonas ; Flagella ; Glyco-proteins ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The flagellar glycoproteins exposed on Chlamydomonas eugametos gametes were labeled by means of lactoperoxidase, diiodosulfanilic acid and chloramine T, and characterised in SDS-electrophoresis gels. The medium from gamete cultures contains particles (isoagglutinins) that agglutinate gametes of the opposite mating type. When crude preparations of these particles were subjected to isopycnic centrifugation in a caesium chloride gradient, two bands of particles were found. The lighter, active band consisted of membrane vesicles. The denser, inactive band consisted of cell wall material. The active band had the same glycoprotein composition as membrane vesicles artificially made from isolated flagella. Preparations of glagella were also separated on a caesium chloride cushion into pure flagella and cell wall material. The flagella, but not the cell wall material, isoagglutinated opposite gametes. Again the glycoprotein composition of pure flagella was similar to that of pure isoagglutinin vesicles. No difference was detected between the protein and glycoprotein compositions of flagella and isoagglutinins from both mating types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380095
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  • 2
    Electronic Resource
    Electronic Resource
    An antiserum against a glycoprotein functional in flagellar adhesion between Chlamydomonas eugametos gametes (1982)
    Springer
    Archives of microbiology 131 (1982), S. 241-246 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Agglutination ; Flagella ; Glycoproteins ; Anti-PAS-1.2 serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlamydomonas eugametos gametes agglutinate sexually by their flagellar surfaces. The agglutination factor on mating type minus (mt-) gametes is thought to be a glycoprotein named PAS-1.2. To test this idea, an antiserum was raised against purified PAS-1.2., which reacted with isolated PAS-1.2 (immunoprecipitation tests) and blocked the ability of isolated PAS-1.2 to induce sexual twitching in mt + gametes. When tested with living cells, the antiserum specifically agglutinated mt - gametes and induced a reaction resembling twitching. Mt + flagella were shown to bind the antiserum (indirect immunofluorescence) but much less than mt - gametes. Mt - gametes pretreated with Fab fragments of the antiserum were unable to reproduce sexually, while treated mt + gametes were unaffected. This effect presumably results from the ability of the serum to block mt - sexual agglutination, for mt - isoagglutinin was completely inactivated by the serum, while mt + isoagglutinin was unaffected. It is therefore argued that PAS-1.2 is the in vivo mt - agglutination factor. However it is shown that the antiserum was able to react in vitro not only with PAS-1.2 but with several other proteins in both mt - and mt + flagella.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405886
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation of an antigenic determinant from flagellar glycoproteins of Chlamydomonas eugametos (1983)
    Springer
    Archives of microbiology 135 (1983), S. 311-314 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Sexual reproduction ; Agglutination ; Flagella ; Antigenic determinant ; Glycoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A labeled oligosaccharide fraction, obtained by reductive β-elimination of an extract of minus mating-type flagella of Chlamydomonas eugametos, is shown to contain a disaccharide fraction which is bound by an antiserum raised against the sexual agglutination factor present at the flagellar surface of that species. The fraction contains arabinose and galactose. It is argued that galactose and/or arabinose may be a functional constituent of the agglutination factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413487
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  • 4
    Electronic Resource
    Electronic Resource
    Sex-specific glycoproteins in Chlamydomonas flagella an immunological study (1980)
    Springer
    Archives of microbiology 126 (1980), S. 77-81 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Sex ; Flagella ; Agglutination ; Glycoproteins ; Immunochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To identify mating type-specific glycoproteins associated with the flagellar membrane of Chlamydomonas eugametos, which could be involved in sexual agglutination, antibodies were raised in rabbits against purified gamete flagella of either mating type. The immunoglobulin (Ig) fractions exhibited partial mating-type specificity in agglutinating gametes, in the indirect immunofluorescence test and in the crossed immunoelectrophoresis test. This specificity was strongly enhanced by absorbing the fractions with flagella of the opposite mating type. Absorbed Ig fractions produced a single precipitation line with Triton extracts of gamete flagella in the crossed immunoelectrophoresis technique. On polyacrylamide gel electrophoresis this line appeared to contain two flagellar glycoprotein fractions, PAS 1 and PAS 4. Polyacrylamide gels of flagellar extracts incubated with these Ig fractions, followed by staining with peroxidase-anti-rabbit Ig resulted in the staining of only the PAS 1 and PAS 4 bands, which confirms that these components of the flagellar membrane are mating type-specific antigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421894
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  • 5
    Electronic Resource
    Electronic Resource
    Sexual agglutination factor from Chlamydomonas eugametos (1981)
    Springer
    Planta 153 (1981), S. 362-369 
    Details
    ISSN: 1432-2048
    Keywords: Agglutination (sexual) ; Chlamydomonas ; Flagella ; Glycoprotein and sexual agglutination ; Membrane ; Sexual reproduction (Chlamydomonas)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlamydomonas eugametos gametes can sexually agglutinate via their flagellar surfaces whereas vegetative cells cannot. Therefore, flagellar glycoproteins, present in gamete cells but absent from vegetative cells, were investigated as prospective mt -agglutination factors. They were identified as periodic acid Schiff (PAS) stained bands separated in sodium dodecyl sulphate-polyacrylamide electrophoresis gels. Gamete-specific bands were determined by comparison with equivalent gels of vegetative flagella and by immunological techniques using antisera raised against isolated mt - gamete flagella. Four high molecular weight flagellar glycoproteins proved to be gamete specific (PAS-1.2, PAS-1.3, PAS-3 and PAS-4). They were extracted from flagella by 3 M guanidine thiocyanate, separated in a column of Sepharose 2B, and tested for in vitro agglutination activity on mt + gametes. A single peak of activity was found to be correlated with the presence of the PAS-1.2 band. It is shown that mt - agglutination activity is related to the concentration of this glycoprotein in flagellar membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384255
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