ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Immature green peas were harvested from plants grown in water culture on Johnson's nutrient solution with radioactive chloride. Twenty five g of lyophilized peas were extracted with butanol, the extract chromatographed, first on Sephadex LH 20 and then on silica gel. The radioactive hormone was followed by scintillation counting without the use of any scintillant. The radioactive fractions were taken up in a few μl of chloroform and injected into a gas chromatograph interfaced with a mass spectrometer. Derivatization was not necessary. The electron impact mass spectrum showed the typical peaks of a chloroindolyl-3-acetic acid methyl ester: the molecular ion cluster at m/e 223, 225 and the characteristic indole fragment ions at m/e 164, 166 (M-COOCH3)+. The chlorine atom was assigned to the 4 position of the indole ring after thin layer chromatography of radioactive extract and standards of four different chlorinated indolyl-3-acetic acid methyl esters.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1978.tb04097.x
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