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  • 1985-1989  (3)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Relative changes in incorporation rates of leucine and methionine during starvation survival of two bacteria isolated from marine waters (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 38 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Two copiotrophic Gram-negative bacteria isolated from marine waters, S14 and Vibrio sp. DW1, were examined for changes in the rate of protein synthesis in the initial phase of energy and nutrient deprivation. The incorporation of [3H] leucine into the trichloroacetic acid (TCA)-insoluble material was examined as a method for estimating rates of protein synthesis. The incorporation of methionine was measured and compared with the results of leucine incorporation. Protein synthesis was demonstrated throughout a period of 120 h of starvation. The incorporation rate was related to the time of starvation and decreased subsequent to an initial increase during the first few hours of dormancy. Control experiments with proteinase K and chloramphenicol demonstrated that the labelled amino acids were preferentially incorporated into proteins. It was also demonstrated that the uptake of amino acids was not a rate-limiting step. During the first hours of starvation the ratio of the protein to the dry weight of the S14 cells increased parallel to the increase in the amino acid incorporation rate. The increased activity of the protein-synthesising system during the first hours of nutrient and energy depletion indicates the presence of an active cellular response to the downshift conditions. Furthermore, these findings are consistent with the increased respiratory activity during the first hours of starvation, which has previously been observed for the bacteria examined in this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01739.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Uptake of leucine by a marine Gram-negative heterotrophic bacterium during exposure to starvation conditions (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 45 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The uptake of leucine by S14, an unidentified marine Gram-negative bacterium, was studied during a starvation period of 96 h. The S14 cells displayed two separate uptake systems with different affinities for leucine. The Km values of these systems were 0.76 μM and 20 μM, respectively. The time of exposure to starvation had a marked effect on both uptake systems, not by changing the affinity for leucine, but rather by altering the velocity of uptake (Vmax). A marked increase in the uptake capacity was noted for the high-affinity system, whereas the uptake velocity decreased for the low-affinity system. An osmotic shock treatment resulted in an almost complete loss of substrate binding activity. A gradual recovery of the leucine uptake subsequent to the osmotic shock was observed during a 72-h period of starvation. Separation of the osmotic shock supernatant by gel filtration revealed two proteins, 37 and 44 kDa in size, with leucine binding activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02361.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of cadmium on starved heterotrophic bacteria isolated from marine waters (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 45 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02350.x
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