ISSN:
1573-0603
Keywords:
cell dissociation
;
clonal culture
;
retinal pigmented
;
cell differentiation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The original procedure for the dissociation of chick retinal pigmented epithelium (RPE) has been modified in an attempt to increase the yield and plating efficiency of RPE cells and to obtain a more uniform and reproducible pattern of growth and differentiation in clonal culture. The new procedure allows efficient dissociation of very small amounts of. RPE into single cells and the establishment of clonal cultures from the RPE of individual chick embryos or from individual RPE clones. The dissociated cells attach and spread within a few hours after plating and form typical clones with highly pigmented centres within 2 to 3 wk. Individual clones started from the same material show little variation in growth pattern and provide an excellent model system for the study of the expression of cell differentiation in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01797778
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