ISSN:
1432-1017
Keywords:
Chloroplasts
;
dielectrophoresis
;
surface charge
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Dielectrophoretic coefficients of chloroplasts, untreated and treated with cationized ferritin, have been measured in axisymmetric ac electric fields at different frequencies. The treated chloroplasts have surface charge density 2.4 times smaller than the untreated ones. The dielectrophoretic coefficients are in the range 10-25 F · m2 to 7x10-25 F · m2 for frequencies from 6,000 Hz to 1 MHz. Dielectrophoretic effects have not been observed for frequencies from 1 to 6,000 Hz and from 1 MHz and 10 MHz. The surface charge decrease leads to an increase of 2–3 times in the dielectrophoretic coefficients and slight shift of the dielectrophoretic mobility of lower frequencies. These results may be qualitatively explained on the basis of the existing theories for cell and vesicle dielectrophoresis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00256358
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