ISSN:
1573-1561
Keywords:
Chemotaxis
;
bioassay
;
nematodes
;
Meloidogyne incognita
;
attractants
;
repellents
;
cucumber roots
;
gradients
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A simple, flexible, and quantifiable bioassay for the attraction or repulsion of plant parasitic nematodes to or from root fractions or pure substances is described. Accurate gradients of volatile and nonvolatile substances can be measured and established. The method entails placing the nematodes in narrow agarose tracks such that their movement is essentially linear and the distance a population has traveled away or toward a given substance can be monitored with time. Plastic plates, each containing 10 such tracks, are described. The method is illustrated with second-stage larvae ofMeloidogyne incognita and a volatile attractant and nonvolatile repellent fraction obtained from cucumber roots.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01014831
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