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  • carbon  (1)
  • osmium impregnation  (1)
  • 1985-1989  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Electron microscopy of endothelial cells in culture: III. Freeze-fracturing (1986)
    Springer
    Methods in cell science 10 (1986), S. 37-39 
    Details
    ISSN: 1573-0603
    Keywords: freeze fracturing ; freeze etching ; endothelial monolayers ; platinum ; carbon ; replicas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Endothelial cells can be examined by freeze-fracturing and replication techniques performed on pellets of endothelial cells scraped off their culture substratum or, alternatively, cells grown on pieces of cover glass can be fractured as monolayers. Deep etching can be performed on either preparation provided that the cryoprotectant glycerol is omitted and water, which sublimes away during the etching process, is substituted. The cleaning and handling of the replicas for subsequent viewing in the electron microscope must be performed with care and are described in detail.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404589
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  • 2
    Electronic Resource
    Electronic Resource
    Electron microscopy of endothelial cells in culture: II. Scanning electron microscopy and OTOTO impregnation method (1986)
    Springer
    Methods in cell science 10 (1986), S. 35-36 
    Details
    ISSN: 1573-0603
    Keywords: scanning electron microscopy ; endothelial cells ; osmium impregnation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method is described whereby endothelial cells are treated with several cycles of osmium and thiocarbohydrazide then critical point dried. Cells grown on Formvar-coated gold grids can be examined directly by transmission electron microscopy, cells grown on glass strips are mounted on stubs for scanning electron microscopy and are scanned without further coating with gold or palladium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404588
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    Paper (German National Licenses)
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