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Contamination of thin sections: Some observations on the cause and elimination of “embedding pepper” (1987)

Mollenhauer, Hilton H.

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 5 (1987), S. 59-63

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ISSN: 0741-0581

Keywords: Electron microscopy ; Postsection staining ; Section contamination ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Natural Sciences in General

Notes: A method is described for eliminating a form of contamination called embedding pepper that appears in, or on, thin sections following poststaining in lead citrate. The pepper is present most often in the matrixes of mitochondria, peroxisomes, and red blood cells. Formation of pepper can be prevented by treating the section with 0.5 N HCl or 1.0% ethylenediaminete-traacetic acid (EDTA) for 1-5 min before staining in uranyl acetate or lead citrate.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jemt.1060050106

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