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  • 1
    Electronic Resource
    Electronic Resource
    Photoaffinity labeling of the DDT1 MF-2 cell α1-adrenergic receptor (1985)
    Springer
    Molecular and cellular biochemistry 67 (1985), S. 47-53 
    Details
    ISSN: 1573-4919
    Keywords: α1-adrenergic receptor ; membrane ; photoaffinity ; smooth muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary In this study, we have used an α1-adrenergic receptor photoaffinity ligand, 2-[4-(4-azido-3-iodo-benzoyl)-piperazin-1-yl]-4-amino-6, 7-dimethoxyquinazoline (125I-APD), to label covalently the α1-adrenergic receptor in a smooth muscle cell line. Our results indicate that in the absence of light, (125I)APD binds reversibly to a site in the DDT1 MF-2 cell membranes having pharmacological characteristics of an α1-adrenergic receptor. Following incorporation of (125I)ADP into partially purified membranes a single labeled band of protein with a Mr of 81 000 was visualized by autoradiography following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Incorporation of (125I)-APD into this band was affected by adrenergic agonists and antagonists in a manner consistent with an α1-adrenergic interaction. Prazosin (α1-selective) blocked incorporation of the label into the Mr = 81 000 protein while yohimbine (α2-selective) did not. Of the adrenergic agonists, (−)-epinephrine and (−)-norepinephrine but not (−)-isoproterenol blocked labeling of the Mr − 81 000 protein. We conclude that the ligand binding site of the DDT1 MF-2 cell α1-adrenergic receptor resides in a Mr = 81 000 protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220985
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  • 2
    Electronic Resource
    Electronic Resource
    Glucocorticoid induction of β-adrenergic receptors in the DDT1 MF-2 smooth muscle cell line involves synthesis of new receptor (1987)
    Springer
    Molecular and cellular biochemistry 74 (1987), S. 21-27 
    Details
    ISSN: 1573-4919
    Keywords: glucocorticoid receptor ; β-adrenergic receptor regulation ; smooth muscle ; adenylate cyclase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have shown that glucocorticoids induce the appearance of β2-adrenergic receptors in membranes of the ductus deferens smooth muscle cell line (DDT1 MF-2). A concomitant increase in isoproterenol stimulated adenylate cyclase activity in the absence of exogenously applied GTP was observed as was a significantly increased (p 〈 0.05) sensitivity of the adenylate cyclase system to exogenously applied GTP. However, no significant difference in the maximal velocity of adenylate cyclase between control and steroid treatment was measurable in the presence of sodium fluoride. Induction of β2-adrenergic receptors in DDT1 MF-2 cells is correlated with the presence of steroid receptors (androgen and glucocorticoid) in the cells since estrogens and progesterones had no effect on receptor levels. Finally, utilizing dense amino acid labeling of cells to measure old versus newly synthesized receptor sites by a density shift method, we have documented that glucocorticoid induction of β2-adrenergic receptors involves synthesis of new receptor protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221909
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  • 3
    Electronic Resource
    Electronic Resource
    Nylon 6,6 copolyamides of bis(2-carboxyethyl) methylphosphine oxide (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 35 (1988), S. 215-227 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Copolyamides of nylon 6,6 with bis(2-carboxyethyl)methylphosphine oxide (CEMPO) were prepared by melt polycondensation of nylon 6,6 salt with CEMPO and hexamethylene diamine. The effect of CEMPO upon melting point, moisture regain, boiling water shrinkage, water wicking, tensile properties, thermal stability, static dissipation, and flammability of nylon 6,6 fibers and fabrics was determined. The fiber properties were greatly affected by the high water absorption and solubility characteristics of the phosphine oxide linkage. However, crystallinity and orientation were not greatly disturbed by up to 30-40M% CEMPO substitution in nylon 6,6. The copolyamides were of improved flame resistance compared to nylon 6,6 and were also found to give improved flame resistance in blends of the copolymer with various commercial plastics. In addition, CEMPO and the corresponding diamine, bis(3-aminopropyl)methylphosphine oxide were used to prepare a series of cycloaliphatic and aromatic ring-containing polyamides, but problems were encountered with moisture sensitivity and low melting points with some of these polymers.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1988.070350116
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  • 4
    Electronic Resource
    Electronic Resource
    Biocompatibility of Delrin 150: A creep-resistant polymer for total joint prostheses (1985)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 19 (1985), S. 519-533 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: A thermoplastic polymer, Delrin 150 (polyoxymethylene homopolymer), with creep resistance ten times that of ultrahigh-molecular-weight polyethylene, is used as a material for total joint protheses. A study was made of the local and systemic host response to this polymer when implanted in three different mammalian species. 316 LC stainless steel was used as a control. The materials were implanted into muscle and bone as solid cylinders. A total of 446 samples were implanted into 74 animals. The duration of implantation ranged from 2 weeks to 2 years. A semiquantitative evaluation of local tissue reaction was performed. For each implant, 16 histological criteria were graded for severity of host tissue reaction. The liver, spleen, kidneys, pancreas, and lungs from each animal were also studied for evidence of systemic toxicity. The polymer implants exhibited a mild tissue reaction with the same characteristics as the control. Local tumor formation, bone osteolysis, and surrounding muscle necrosis were not seen. No pathological changes compatible with systemic toxicity by Delrin 150 were observed in the study of the organs. Delrin 150 in solid form did not exhibit local or systemic toxicity and is therefore biocompatible by this study. Powder implantation studies should be performed to simulate tissue response to wear particles.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820190505
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