ISSN:
1432-2048
Keywords:
Nicotiana
;
Pollen (culture, development)
;
Pollen maturation (in vitro)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct stages of development, including the microspore stage. In in-vitro cultures, fully functional, mature pollen was obtained. In a germination medium, this pollen produced pollen tubes. After application to stigmas in situ, the in-vitro-matured pollen fertilized ovules, and seeds were produced. Genetic tests with seedlings obtained from pollinations with in-vitro-matured pollen from a transgenic plant revealed normal Mendelian segregation of two marker genes, the neomycin-phosphotransferase II gene and the nopaline-synthase gene. These results are of interest with respect to the control of self-incompatibility, cytoplasmic male sterility and pollen-allergen formation, and it offers an alternative route for gene transfer in those plants which cannot be regenerated in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392439
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