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  • Microtubule  (2)
  • SOLAR PHYSICS  (2)
  • Female
  • 1985-1989  (4)
  • 1
    ISSN: 1615-6102
    Keywords: Immunofluorescence ; Maize leaf ; Microtubule ; Protoplast culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Maize (Zea mays) leaf protoplasts were isolated from various leaves of two-week (4-leaf) seedlings and from sections of the third leaf blades. Microtubules (MTs) were visualized using immunofluorescence microscopy. Only freshly isolated protoplasts from the third and fourth leaf blades contained MTs, with protoplasts from the fourth leaf containing the most i.e. 13% of fourth-leaf protoplasts contained MTs. In general, protoplasts with fewer and smaller chloroplasts had more MTs. Initially 90–95% of protoplasts from basal portions of leaves had MTs but the percentage decreased slightly during culture particularly after 10 days. The antioxidant n-propyl gallate was beneficial in maintaining MT content. Few protoplasts from older sections intitially contained MTs but in all sections at least some protoplasts regained a significant MT content during culture (e.g., 10% of protoplast from the tip section possessed microtubules after 7 days of culture). Far fewer MTs were observed in individual leaf protoplasts than those isolated from suspension culture.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 150 (1989), S. 110-116 
    ISSN: 1615-6102
    Keywords: Microtubule ; Nuclear number ; Preprophase band ; Soybean protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Multinucleate cells derived from soybean protoplasts were used to investigate the effect of increased nuclear number on the development and frequency of preprophase bands (PPBs) of microtubules (MTs). The results do not support the assumption that one nucleus establishes one PPB because the majority of multinucleate cells had only one large PPB. However, nuclear number or ploidy level has some influence on PPB development since double PPBs occurred more often in multinucleate than uninucleate cells. Double (divergent) PPBs were present at early and late stages of PPB development, suggesting that they are not a transient stage. PPBs in multinucleate cells developed in a similar fashion to those in uninucleate cells. In multinucleate cells, each dividing nucleus had its own spindle and phragmoplast. Subsequent phragmoplast development was frequently uncoupled from PPB distribution. Most multinucleates contained a single large PPB but at telophase, multiple phragmoplasts oriented in different planes.
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  • 3
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    In:  Other Sources
    Publication Date: 2019-07-12
    Description: The proper motion of magnetic elements on the quiet sun has been measured by means of local correlation tracking. The existence of a pattern in the intranetwork (IN) flow is confirmed. This velocity field is consistent with the direct Doppler measurement of the horizontal component of the supergranular velocity field. The IN elements generally move toward the network boundaries. By tracking test points, it is confirmed that the magnetic elements converge in areas corresponding to the magnetic network. But because the IN elements are of random polarity, they cannot contribute to the growth or maintenance of the magnetic network. By calculating the cross correlation between the magnetogram and Dopplergram, it is confirmed that the supergranule boundaries and the magnetic network are roughly correlated.
    Keywords: SOLAR PHYSICS
    Type: Solar Physics (ISSN 0038-0938); 115; 2 19
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  • 4
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    In:  Other Sources
    Publication Date: 2019-07-12
    Description: The contrast of solar faculae near the limb is measured on direct video images made with the 65 cm vacuum reflector at the Big Bear Solar Observatory. Six broad band filters with different wavelengths from red to violet are used. About 300 images were measured from observations made during the summers of 1983 and 1985. Over 20,000 faculae were measured. By averaging the contrast of faculae and plotting them vs heliocentric angle, it is found that contrast increases monotonically toward the limb for the shorter wavelengths; for longer wavelengths, contrast has a tendency to peak around mu = 0.15, and then decrease toward the extreme limb. The contrast increases as wavelength decreases.
    Keywords: SOLAR PHYSICS
    Type: Solar Physics (ISSN 0038-0938); 110; 2 19
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