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  • 1
    Electronic Resource
    Electronic Resource
    Acquisition of embryogenic potential in carrot cell-suspension cultures (1988)
    Springer
    Planta 176 (1988), S. 196-204 
    Details
    ISSN: 1432-2048
    Keywords: Cell suspension culture ; Daucus ; Embryogenic potential ; Excreted cell factor ; Gene expression mRNA (in vitro translation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenic suspension cultures of domesticated carrot (Daucus carota L.) are characterized by the presence of proembryogenic masses (PEMs) from which somatic embryos develop under conditions of low cell density in the absence of phytohormones. A culture system, referred to as starting cultures, was developed that allowed analysis of the emergence of PEMs in newly initiated hypocotyl-derived suspension cultures. Embryogenic potential, reflected by the number of FEMs present, slowly increased in starting cultures over a period of six weeks. Addition of excreted, high-molecular-weight, heat-labile cell factors from an established embryogenic culture considerably accelerated the acquisition of embryogenic potential in starting cultures. Analysis of [35S]methionine-labeled proteins excreted into the medium revealed distinct changes concomitant with the acquisition of embryogenic potential in these cultures. Analysis of the pattern of gene expression by in-vitro translation of total cellular mRNA from starting cultures with different embryogenic potential and subsequent separation of the [35S]methionine-labeled products by two-dimensional polyacrylamide gel electrophoresis demonstrated a small number of abundant in-vitro-translation products to be present in somatic embryos and in embryogenic cells but absent in nonembryogenic cells. Several other in-vitro-translation products were present in explants, non-embryogenic and embryogenic cells but were absent in somatic embryos. Hybridization of an embryoregulated complementary-DNA sequence, Dc3, to RNA extracted from starting cultures showed that the corresponding gene is expressed in somatic embryos and PEMs but not in non-embryogenic cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392445
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  • 2
    Electronic Resource
    Electronic Resource
    Synthese von 1,2,4-Benzotriazin-3-ylessigsäureestern und-amidenXI. Mitt. der Reihe: Oxidative Kupplung CH-acider Verbindungen mit p-Phenylendiaminen. (1985)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 327 (1985), S. 297-309 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Synthesis of 1,2,4-Benzotriazine-3-acetates and -acetamidesSubstituted 1,2,4-benzotriazine-3-acetates 6 are prepared by the reduction of (2-nitrophenyl)hydrazonoethers 4 or (2-nitrophenyl)amidrazones 5. The 1,2,4-benzotriazin-3-ylacet-anilid 9g forms a yellow azomethine dye 10 by oxidative coupling with N,N-diethyl-p-phenylenediamine.
    Additional Material: 8 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19853270215
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  • 3
    Electronic Resource
    Electronic Resource
    Oxidative Kupplung von 5-Amino-pyrazolen mit p-PhenylendiaminenXII. Mitt. der Reihe „Oxidative Kupplung CH-acider Verbindungen mit p-Phenylendiaminen“, XI. Mitt. siehe [1]. (1986)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 328 (1986), S. 342-348 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Oxidative Coupling of 5-Aminopyrazoles with p-PhenylenediaminesBy oxidative coupling of 5-amino-3-methyl-1-phenyl-pyrazole 4 with N,N-diethyl-p-phenylenediamine the 4-(4-N,N-diethylamino-phenylimino)-3-methyl-1-phenyl-2-pyrazolin-5-imin 5 is formed. 5 is oxidized very easily to the 1H-pyrazolo[3,4-b]quinoxaline 6a. The Z/E-isomerism of the azomethine dye is investigated by dynamic n.m.r.-spectroscopy. 1H-Pyrazolo[3,4-b]quinoxalines (6a-d) are also formed by reaction of 1,3-disubstituted 5-aminopyrazoles with p-nitroso-dialkylanilines.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19863280308
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