ISSN:
1432-0983
Keywords:
Alcoholic fermentation
;
Deletion mutant
;
Pyruvate decarboxylase
;
Yeast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We deleted most of the pyruvate decarboxylase structural gene PDC1 from the genome of Saccharomyces cerevisiae. Surprisingly, mutants carrying this deletion allele showed a completely different phenotype than previously described point mutations. They were able to ferment glucose and their specific pyruvate decarboxylase activity was only reduced to 45% of the wild type level. Northern blot analysis revealed that a sequence in the yeast genome homologous to PDC1 and formerly designated as a possible pseudogene is expressed and may code for a different but closely related pyruvate decarboxylase. The products of the two PDC genes seem to form hybrid oligomers, however both homooligomers have enzyme activity. Thus, the product of the PDC1 gene is not absolutely neccessary for glucose fermentation in yeast.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00435452
Permalink
