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  • Chromosome identification  (1)
  • Life and Medical Sciences  (1)
  • Monocotyledonous plants  (1)
  • 1985-1989  (3)
Collection
Keywords
Publisher
Years
  • 1985-1989  (3)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Identification of the 21 monosomic lines in Avena byzantina C. Koch cv. ‘Kanota’ (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 271-278 
    Details
    ISSN: 1432-2242
    Keywords: Oats ; Monosomic series ; Nullisomics ; Avena byzantina ; Chromosome identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary All of the 21 possible monosomic lines have been screened and confirmed from 33 monosomic stocks of Avena byzantina C. Koch cv. ‘Kanota’. All of them, except Mono-21 which was a progeny of monosomic ‘Cherokee’ (A. sativa) repeatedly backcrossed with ‘Kanota’, were obtained in the progenies of haploid (2n = 3x), aneuploid (2n = 6X±) and autotriploid (2n = 9X) partners of twins. Identification of the monosomics was carried out by means of the double monosomic method, monosomic analysis on marker genes, leaf peroxidase isozyme analysis, karyotype analysis and nullisomic analysis. The monosomic lines were numbered from Mono-1 through to Mono-21, mainly in the order of monosome length from the longest to the shortest. Most monosomic lines were hardly distinguishable by morphological characteristics from each other or from normal disomics. In the selfed progenies of four monosomic lines, Mono-8, -9, -17 and-19, segregation of nulli-, mono- and disomics was observed, but no nullisomics were found in the other lines. In most cases the frequency of monosomics ranging from 35.5 to 97.8% was, compared to those of nulli- and disomics, highest in the selfed progeny of monosomics. The monosomic lines were easily maintained and can be used for genetic analysis because of their good seed fertility and high monosome transmission rate. They have the near isogenic background of ‘Kanota’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304911
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  • 2
    Electronic Resource
    Electronic Resource
    Absence in monocotyledonous plants of the diffusible plant factors inducing T-DNA circularization and vir gene expression in Agrobacterium (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 221-226 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Monocotyledonous plants ; Plant factors ; T-DNA circularization ; vir gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary T-DNA circularization is one of the molecular events specifically induced in agrobacterial cells upon their infection of dicotyledonous plant cells. We developed a seedling co-cultivation procedure to determine whether or not monocotyledonous plants have the ability to induce T-DNA circularization and vir gene expression. Co-cultivation of Agrobacterium tumefaciens with seedlings of dicotyledonous plants showed that the circularization event takes place efficiently. The exudates and extracts of the seedlings also effectively induced T-DNA circularization and vir gene expression, indicating that dicotyledonous seedlings contain diffusible factors capable of inducing these molecular events. In contrast, neither T-DNA circularization nor vir gene expression was detectable when Agrobacterium was incubated with seedlings of monocotyledonous plants. Supplementing with acetosyringone, a known inducer of vir gene expression and T-DNA circularization, resulted in the induction of circularization during co-cultivation with monocotyledonous seedlings. These results indicate that the seedlings of monocotyledonous plants have no detectable amounts of diffusible inducers, unlike dicotyledonous seedlings. Therefore, it is unlikely that the vir genes are expressed in Agrobacterium inoculated in monocotyledonous plants. This may be one of the blocks in tumorigenesis of monocotyledonous plants by Agrobacterium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329646
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  • 3
    Electronic Resource
    Electronic Resource
    Sensitivity of preadipose 3T3 cells to growth hormone (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 128 (1986), S. 293-298 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cultured preadispose 3T3 cells are able to undergo a process of differentiation through which they are converted into adipose cells. Growth hormone induces this conversion in resting cultures but not in growing cultures. It was of interest to determine the period of cell sensitivity to the hormone and the timing of the induction of glycerophosphate dehydrogenase, a key enzyme in lipogenesis. It was found that 3T3-F442A cells became highly sensitive to rat growth hormone at confluence but that high sensitivity remained for only 3 days; thereafter, the responsiveness to the rat growth hormone declined rapidly. Refeeding of the cells with fresh medium did not lead to the recovery of the hormone sensitivity, indicating that the decrease in sensitivity was not due to exhaustion of medium components but that it seemed to be a specific property of F442A cells. As glycerophosphate dehydrogenase activity was detected at nearly the same time as its mRNA was measurable, it is likely that the mRNA is translated immediately after its synthesis.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041280222
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