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Influence of E. coli lipopolysaccharide binding to rat alveolar type II cells on their functional properties (1985)

Aracil, F. M. ; Bosch, M. A. ; Municio, A. M.

Springer

Molecular and cellular biochemistry 68 (1985), S. 59-66

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ISSN: 1573-4919

Keywords: endotoxic shock ; lipopolysaccharide ; pneumocytes

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: Summary The interaction between lipopolysaccharide from E. coli0111:B4 and rat alveolar type II pneumocytes and its influence on the functional properties of the cells and their membranes were studied. Type II cells were isolated by a novel procedure involving digestion of the lung connective tissue with elastase and Percoll-gradient centrifugation. Binding of (14C)lipopolysaccharide to type II cells resulted in a partially reversible, non-specific, high affinity process. (l4C)Choline incorporation into phosphatidylcholine by type II cells was stimulated by lipopolysaccharide, the maximum effect being observed at 10–20 μg/ml. 45Ca2+ uptake by type II cells was also increased by lipopolysaccharide. Using plasma membranes from lung homogenates an increase of membrane microviscosity versus the amount of lipopolysaccharide was shown. These results indicate that E. coli lipopolysaccharide interacts with alveolar type 11 cells by binding reversibly to particular ingredients of the membrane bilayer and induces a modification of ion permeability and fluidity of the membrane.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00219389

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Microsomal membrane fluidity and phosphatidylcholine synthesis in rabbit lung under high oxygen tension (1989)

Casals, Cristina ; Herrera, Luz ; Gasset, Maria ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 7 (1989), S. 193-199

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ISSN: 0263-6484

Keywords: Lung ; phosphatidylcholine ; lysolecithin acyltansferase ; membrane fluidity ; hyperoxemia ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Phosphatidylcholine metabolism and membrane fluidity were studied in microsomes isolated from rabbit lung, which had been exposed to high oxygen tension for 30 min.In these microsomes the incorporation of [3H]-palmitate into phosphatidylcholine increased whereas the incorporation of [14C]-glycerol and [14C]-choline from CDP-[methyl-14C]-choline remained unchanged in comparison to the control microsomes. The enhanced [3H]-palmitate incorporation may be explained by an increase of the specific activity of acyl-CoA:lysophosphatidylcholine acyltransferase which was measured in microsomes from hyperoxic lung.Although microsomal parameters influencing membrane fluidity, such as the cholesterol/phospholipid molar ratio, unsaturation degree of phospholipid acyl chains and lipid/protein ratio, are altered after oxygen treatment in vivo, no change of fluorescence polarization (PDPH) and lipid structural order parameter (SDPH) could be measured. Probably, the membrane maintains its fluidity by counteracting effects on different factors on which the fluidity depends.

Additional Material: 4 Tab.