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  • Chemistry  (2)
  • cDNA sequence  (1)
  • 1985-1989  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of cDNA clones coding for spinach nitrite reductase: Complete sequence and nitrate induction (1988)
    Springer
    Molecular genetics and genomics 212 (1988), S. 20-26 
    Details
    ISSN: 1617-4623
    Keywords: Nitrite reductase ; cDNA sequence ; Nitrate assimilation ; Nitrate induction ; Transit peptide sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The main nitrogen source for most higher plants is soil nitrate. Prior to its incorporation into amino acids, plants reduce nitrate to ammonia in two enzymatic steps. Nitrate is reduced by nitrate reductase to nitrite, which is further reduced to ammonia by nitrite reductase. In this paper, the complete primary sequence of the precursor protein for spinach nitrite reductase has been deduced from cloned cDNAs. The cDNA clones were isolated from a nitrate-induced cDNA library in two ways: through the use of oligonucleotide probes based on partial amino acid sequences of nitrite reductase and through the use of antibodies raised against purified nitrite reductase. The precursor protein for nitrite reductase is 594 amino acids long and has a 32 amino acid extension at the N-terminal end of the mature protein. These 32 amino acids most likely serve as a transit peptide involved in directing this nuclearencoded protein into the chloroplast. The cDNA hybridizes to a 2.3 kb RNA whose steady-state level is markedly increased upon induction with nitrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00322440
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of surface pretreatments of carbon fiber-polyimide matrix composites (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 14 (1989), S. 496-504 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Various surface pretreatments of carbon fiber-polyimide matrix composites have been studied by XPS, ISS, PASFTIR (photoacoustic Fourier transform infrared spectroscopy) and high-resolution SEM. Reduction of fluorocarbons from release cloth contamination and increase in carbon-oxygen-containing functional groups was observed on the surface of composite samples treated in an oxygen plasma. These plasma-treated samples also showed differences in surface topography, but no change in the photoacoustic infrared spectra when compared to a methanol-washed sample.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740140903
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  • 3
    Electronic Resource
    Electronic Resource
    Reaction rates for nucleophiles with cyanogen chloride: Comparison with two other digonal carbon compounds (1986)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 18 (1986), S. 165-180 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The hydrolysis kinetics of CICN have been reinvestigated from pH 0.0-10.5 and from 18-40°C. In the pH range from 1-5, the hydrolysis rate is invariant and the activation parameters (ΔH
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550180204
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