Publication Date:
1988-07-01
Description:
High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant approximately equal to 1.0 nM) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity with estrogen binding. RNA blot analysis with a complementary DNA probe to the human estrogen receptor revealed putative receptor transcripts of 6 to 6.2 kilobases in both rat and human osteosarcoma cells. Type I procollagen and transforming growth factor-beta messenger RNA levels were enhanced in cultured human osteoblast-like cells treated with 1 nM estradiol. Thus, estrogen can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Komm, B S -- Terpening, C M -- Benz, D J -- Graeme, K A -- Gallegos, A -- Korc, M -- Greene, G L -- O'Malley, B W -- Haussler, M R -- New York, N.Y. -- Science. 1988 Jul 1;241(4861):81-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, University of Arizona College of Medicine, Tucson 85724.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3164526" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Binding, Competitive
;
Cell Nucleus/metabolism
;
DNA/genetics
;
Estradiol/*metabolism/pharmacology
;
Humans
;
Iodine Radioisotopes
;
Nucleic Acid Hybridization
;
Osteoblasts/drug effects/*metabolism
;
Osteosarcoma/*metabolism
;
Peptides/genetics
;
Procollagen/genetics
;
RNA, Messenger/*metabolism
;
Rats
;
Receptors, Estrogen/genetics/*metabolism
;
Transcription, Genetic
;
Transforming Growth Factors
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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