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  • GEOPHYSICS  (1,103)
  • Biochemistry and Biotechnology  (487)
  • 1985-1989  (1,590)
  • 1
    Electronic Resource
    Electronic Resource
    Vanadium containing bromoperoxidase: An example of an oxidoreductase with high operational stability in aqueous and organic mediaSend all correspondence to Ms. G. E. E. van Noppen, F.I.L., Publications Secretary, Laoratory of Biochemistry, University of Amsterdam, P.O. Box 20151, 1000 HD Amsterdam, The Netherlands. (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 607-610 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The conversion is described of phenolsulphonephtalein (phenol red) to 3,3′,5,5′-tetrabromophenolsulphonephthalein (bromophenol blue) by bromoper-oxidase from the brown alga Ascophyllum nodosum. This reaction provides a convenient assay for the detection of bromoperoxidase activity in vitro. Bromoperoxidase was shown to be stable under turnover conditions for three weeks at room temperature, catalyzing the bromination of phenol red into bromophenol blue. When stored at room temperature in organic sol vents such as acetone, methanol, ethanol [present up to 60% (v/v)], and 1-propanol [40% (v/v)], bromoperoxidase was stable for more than one month. As far as we know this is the first example of an oxidoreductase which displays such great stability. This enhances the applicability of the enzyme in organic synthesis.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260300504
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  • 2
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    Measurements of nitric oxide and total reactive nitrogen in the Antarctic stratosphere - Observations and chemical implications (1989)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: Results are presented on measurements of NO and the sum of reactive nitrogen species, NO(y), which include NO, NO2, NO3, N2O5, HNO3, and ClONO2 (in addition to ClO, O3, H2O, and N2O measurements), obtained aboard the NASA ER-2 aircraft flying over the Antarctica between the latitudes of 53 and 72 deg S during the Airborne Antarctic Ozone Experiment. The boundary of the chemically perturbed region (CPR), as indicated by a sharp increase in the level of ClO, occurred near 66 deg S; outside or equatorward of the CPR, the NO(y) mixing ratios ranged between 6 and 12 ppbv, with values decreasing poleward and reaching total NO(y) levels of 4 ppbv or less within 5-deg poleward of the boundary. Data presented in this paper clearly associate the Antarctic ozone decrease with perturbed conditions of ClO, NO(y), and H2O, which are in turn associated with processes defined as nonstandard heterogeneous chemistry, denitrification, and dehydration, respectively.
    Keywords: GEOPHYSICS
    Type: Journal of Geophysical Research (ISSN 0148-0227); 94; 16665-16
    Format: text
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    NASA TECHNICAL REPORTS
  • 3
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    Implications of AAOE observations for proposed chemical explanations of the seasonal and interannual behavior of Antarctic ozone (1989)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: Model simulations were used to investigate the seasonal and interannual behavior of ozone for different choices of initial odd nitrogen concentration in July and different assumptions on the heterogeneous reactions, with particular consideration given to the possible contribution of chlorine chemistry to the ozone hole phenomenon. The numerical experiments were selected based on the simulations of the observed trace gas concentrations during the Airborne Antarctic Ozone Experiment in 1987. In all cases considered, the catalytic cycle associated with the formation and photolysis of Cl2O2 could account for more than half of the photochemical removal of O3 within the Antarctic vortex through mid-September. The reaction of BrO with ClO, which accounts for 15-20 percent of O3 removal in the same period, tends to play a more important role toward the end of September, when the concentration of ClO is expected to decrease. No simple relationship was found between the increase in chlorine lavel and the interannual decrease in Antarctic O3.
    Keywords: GEOPHYSICS
    Type: Journal of Geophysical Research (ISSN 0148-0227); 94; 16705-16
    Format: text
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    NASA TECHNICAL REPORTS
  • 4
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    Development of Archean crust in the Wind River Mountains, Wyoming (1986)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: The Wind River Mountains are a NW-SE trending range composed almost entirely of high-grade Archean gneiss and granites which were thrust to the west over Phanerozoic sediments during the Laramide orogeny. Late Archean granites make up over 50% of the exposed crust and dominates the southern half of the range, while older orthogneisses and magnatites form most of the northen half of the range. Locally these gneisses contain enclaves of supracrustal rocks, which appear to be the oldest preserved rocks in the range. Detailed work in the Medina Mountain area of the central Wind River Mountains and reconnaissance work throughout much of the northern part of the range has allowed definition of the sequence of events which marked crustal development in this area. The sequence of events are described.
    Keywords: GEOPHYSICS
    Type: Lunar and Planetary Inst. Workshop on Early Crustal Genesis: The World's Oldest Rocks; p 41-45
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 5
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    SAGE II aerosol correlative observations - Profile measurements (1989)
    In:  Other Sources
    Details
    Publication Date: 2019-07-12
    Description: Profiles of the aerosol extinction measurements from the Stratospheric Aerosol and Gas Experiment (SAGE) II are compared with profiles from five correlative experiments between November 1984 and July 1986. The correlative profiles were derived from six-channel dustsonde measurements and two-wavelength lidar backscatter data. The correlation between the dustsonde- and lidar-derived measurements and the SAGE II data is good, validating the SAGE II lower stratospheric aerosol extinction measurements.
    Keywords: GEOPHYSICS
    Type: Journal of Geophysical Research (ISSN 0148-0227); 94; 8353-836
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
    Electronic Resource
    Electronic Resource
    Simulation of the growth pattern of a single cell of Escherichia coli under anaerobic conditions (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1027-1035 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A computer model based on a previous model for aerobic growth of Escherichia coli is described which simulates cell composition, size, and shape; length of C and D periods; cell yields; and the rate of product formation of anaerobically grown cells of E. coli B/r-A on glucose-limited minimal medium. To verify the simulation results, the values of cell volume, cell content of DNA, RNA, and protein, substrate yield, ATP yield, and fermentation products for various growth rates were obtained experimentally. Model predictions are in good agreement with experimental results. Such agreement supports a hypothesis that only those equations describing energy metabolism need to be modified and other cell functions are not grossly altered by a switch from aerobic to an aerobic growth. The model's ability to predict reasonable growth responses under anaerobic conditions by only modifying energy metabolism is a further indication of the robust nature of the description of cell physiology included in the development of the aerobic model.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260270714
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  • 7
    Electronic Resource
    Electronic Resource
    Simulation of CFSTR through development of a mathematical model for anaerobic growth of Escherichia coli cell population (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1051-1055 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A computer model is described that simulates a population of Escherichia coli B/r-A cells growing under anaerobic conditions. This population model is an ensemble of single-cell models. The ability of the model of predict the dynamic response of a cell population in a CFSTR to a change in feed flowrates or concentrations was investigated. With glucose as the limiting nutrient the feed concentration of glucose was shifted from 1.0 to 1.88 g/L. With a fixed concentration of glucose (1.0 g/L) step changes in residence time (4.1-1.95 h) were examined. The predicted changes of cell size distribution, substrate concentration, RNA content, and cell dry weight during the transition period compared reasonably well to those observed experiementally. We believe this model is the only model currently available that can make such predictions on an a priori basis.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260270717
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  • 8
    Electronic Resource
    Electronic Resource
    Lipase-catalyzed ester exchange reactions in organic media with controlled humidity (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 258-266 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Immobilized lipase activity is studied in organic solvent systems of controlled water content under the influence of a variety of reaction parameters, such as temperature, relative humidity, substrate concentrations, and type of fatty acid used. Control of the amount of water in the reaction system was found to be a valuable tool for the orientation of the reaction process and for the determination of the final reaction products. The properties of the immobilized lipase were studied using the interesterification of triolein and palmitic acid as the model system.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260300216
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular regulation of methanol oxidase activity in continuous cultures of Hansenula polymorpha (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 32 (1988), S. 577-583 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The regulation of methanol oxidase (MOX) in Hansenula polymorpha has been studied in continuous cultures using a mixture of glucose and methanol (4:1 w/w) as carbon source. The study focused on the identification of stages in the biosynthesis affecting the formation of active MOX in glucose-methanol-grown cells. The levels of MOX mRNA, MOX protein in monomeric and octameric from, the ratio FAD/MOX, and the actual MOX activity have been quantified as functions of the dilution rate D. Hybridization studies with MOX mRNA probes showed an induction of MOX mRNA formation up to D = 0.29 h-1. The induction of MOX protein synthesis (up to 37% of the cellular protein) is determined at low D values on the transcriptional level. The MOX activity at high D values is tuned by FAD incorporation and (post-) translation. Despite the high levels of MOX mRNA, decreasing levels of MOX activity and MOX protein were found at D values ranging from 0.14 t 0.29 h-1. The maximal ratio FAD/MOX(6) was determined at D = 0.1 h-1, which correlated with the maximal specific activity of MOX. In glucose-methanol media both protein level and MOX activity are repressed by increasing levels of residual glucose at high D values.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260320502
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  • 10
    Electronic Resource
    Electronic Resource
    The degradation of lignocellulosics by extremely thermophilic microorganisms (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 1495-1499 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Five extremely thermophilic cellulose-degrading isolates obtained from New Zealand thermal springs were tested for their ability to degrade a number of natural lignocellulosic substrates. Degradation by three of the isolates was generally similar to that by the moderate thermophile, Clostridium thermocellum but occurred at a higher temperature. The New Zealand isolates were also found to grow on xylan as sole carbohydrate source, which probably extends their attack to hemicellulose.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260331118
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