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  • Articles  (16)
  • Engineering General  (8)
  • Biochemistry and Biotechnology  (5)
  • Male
  • 1985-1989  (16)
  • 1
    Electronic Resource
    Electronic Resource
    Surface immobilization of plant cells (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 293-299 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A novel technique has been developed to immobilize plant cells. The cells are deposited on a surface of manmade fibrous material that provides for strong binding of the plant tissue biomass growing in the submerged culture. The immobilized plant cells remain fully viable. Relatively uniform biomass loadings of up to 20 mg d.w. plant cells/cm2 support material have been attained. All plant cells from the inoculum suspension became attached within the first 24-48 h depending on the support matrix configuration and hydraulic culture conditions. The advantages and scale-up potential of this technique are discussed and compared to other culturing modes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260330307
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Bacterial adsorption to smooth surfaces: Rate, extent, and spatial pattern (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1662-1667 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of bulk-water bacterial cell concentration and specific growth rate history on bacterial adsorption rates to surfaces was investigated using response surface analysis. A pure culture of Pseudomonas sp. 224S was grown in a chemostat and pumped into a continuous flow reactor where the bacteria were exposed to clean, glass surfaces under turbulent flow conditions for a period of six hours. Adsorption rate decreased approximately linearly with increasing specific growth rate history. Glass surfaces became saturated with 224S at ca. 0.1% coverage and the resulting spatial pattern of the adsorbed cells deviated from random in the direction of uniformity.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260271206
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  • 3
    Electronic Resource
    Electronic Resource
    Activity of Pseudomonas aeruginosa in biofilms: Effect of calcium (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 406-414 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aerobic glucose metabolism by Pseudomonas aeruginosa biofilms at various calcium loading rates was investigated. The influence of calcium on specific growth rate, extracellular polymeric substance (EPS) formation rate, biofilm detachment rate, and biofilm calcium concentrations was determined. Calcium accumulated in the biofilm in proportion to the liquid phase concentration. Increasing calcium concentration increased the cohesiveness of the biofilm as indicated by a lower relative detachment rate. Specific activity in the biofilm was the same as that measured in a chemostat and was not influenced by changing calcium concentration. EPS formation rate in the biofilm was unaffected by calcium concentration but was higher than that observed in a chemostat.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260330405
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  • 4
    Electronic Resource
    Electronic Resource
    Routine luminescence immunoassays - dream or reality (1989)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 4 (1989), S. 79-87 
    Details
    ISSN: 0884-3996
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170040114
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  • 5
    Electronic Resource
    Electronic Resource
    Conservative calculations of non-isentropic transonic flows (1985)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 5 (1985), S. 1047-1057 
    Details
    ISSN: 0271-2091
    Keywords: Transonic Flow ; Modified Potential ; Finite Elements ; Non-isentropic Flow ; Conservative Method ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The classical potential formulation of inviscid transonic flows is modified to account for non-isentropic effects. The density is determined in terms of the speed as well as the pressure, which in turn is calculated from a second-order mixed-type equation derived via differentiating the momentum equations.The present model differs in general from the exact inviscid Euler equations since the flow is assumed irrotational. On the other hand, since the shocks are not isentropic, they are weaker and are placed further upstream compared to the classical potential solution. Furthermore, the streamline leaving the aerofoil does not necessarily bisect the trailing edge.Results for the present conservative calculations are presented for non-lifting and lifting aerofoils at subsonic and transonic speeds and compared to potential and Euler solutions.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650051204
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  • 6
    Electronic Resource
    Electronic Resource
    Finite element stream function-vorticity solutions of the incompressible Navier-Stokes equations (1987)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 7 (1987), S. 17-27 
    Details
    ISSN: 0271-2091
    Keywords: Finite Element ; Navier-Stokes Stream ; Function Vorticity ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The incompressible, two-dimensional Navier-Stokes equations are solved by the finite element method (FEM) using a novel stream function/vorticity formulation. The no-slip solid walls boundary condition is applied by taking advantage of the simple implementation of natural boundary conditions in the FEM, eliminating the need for an iterative evaluation of wall vorticity formulae. In addition, with the proper choice of elements, a stable scheme is constructed allowing convergence to be achieved for all Reynolds numbers, from creeping to inviscid flow, without the traditional need for upwinding and its associated false diffusion. Solutions are presented for a variety of geometries.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650070103
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  • 7
    Electronic Resource
    Electronic Resource
    On the computation of weakly separated laminar flows in a smoothly expanding channel (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Communications in Applied Numerical Methods 3 (1987), S. 327-333 
    Details
    ISSN: 0748-8025
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In Reference 1, a summary of results of numerical solutions to steady-state laminar flows in an expanding planar channel from 15 research groups was presented. Both vorticity and pressure values computed along a wall of the channel were compared. This paper presents some possible explanations for the wide discrepancies in these computed results.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cnm.1630030415
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  • 8
    Electronic Resource
    Electronic Resource
    Direct solution procedure for solution of harmonic problems using complete, non-singular, Trefftz functions (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Communications in Applied Numerical Methods 5 (1989), S. 159-169 
    Details
    ISSN: 0748-8025
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Boundary approximation solutions using a singular Green function basis have been widely applied to the solution of a variety of linear problems. To avoid the difficulties associated with integration over singularities it is convenient to use sets of non-singular, complete Trefftz functions, and this procedure has been applied recently with success using an indirect formulation. In the paper we observe that direct formulation is here again possible and that for some problems it performs in a superior way.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cnm.1630050304
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  • 9
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    Physical mapping of a translocation breakpoint in neurofibromatosis (1989)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1989-06-02
    Description: The gene for von Recklinghausen neurofibromatosis (NF1), one of the most common autosomal-dominant disorders of humans, was recently mapped to chromosome 17 by linkage analysis. The identification of two NF1 patients with balanced translocations that involved chromosome 17q11.2 suggests that the disease can arise by gross rearrangement of the NF1 locus, and that the NF1 gene might be identified by cloning the region around these translocation breakpoints. To further define the region of these translocations, a series of chromosome 17 Not I-linking clones has been mapped to proximal 17q and studied by pulsed-field gel electrophoresis. One clone, 17L1 (D17S133), clearly identifies the breakpoint in an NF1 patient with a t(1;17) translocation. A 2.3-megabase pulsed-field map of this region was constructed and indicates that the NF1 breakpoint is only 10 to 240 kilobases away from 17L1. This finding prepares the way for the cloning of NF1.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fountain, J W -- Wallace, M R -- Bruce, M A -- Seizinger, B R -- Menon, A G -- Gusella, J F -- Michels, V V -- Schmidt, M A -- Dewald, G W -- Collins, F S -- NS22224/NS/NINDS NIH HHS/ -- NS23410/NS/NINDS NIH HHS/ -- NS23427/NS/NINDS NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1989 Jun 2;244(4908):1085-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, University of Michigan, Ann Arbor 48109.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2543076" target="_blank"〉PubMed〈/a〉
    Keywords: *Chromosome Mapping ; *Chromosomes, Human, Pair 17 ; Cloning, Molecular ; DNA Restriction Enzymes ; Electrophoresis ; Female ; Genetic Linkage ; Humans ; Hybrid Cells ; Male ; Neurofibromatosis 1/*genetics ; *Translocation, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 10
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    Long-term neuropathological and neurochemical effects of nucleus basalis lesions in the rat (1987)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1987-11-13
    Description: The long-term effects of excitotoxic lesions in the nucleus basalis magnocellularis of the rat were found to mimic several neuropathological and chemical changes associated with Alzheimer's disease. Neuritic plaque-like structures, neurofibrillary changes, and neuronal atrophy or loss were observed in the frontoparietal cortex, hippocampus, amygdala, and entorhinal cortex 14 months after the lesions were made. Cholinergic markers in neocortex were reduced, while catecholamine and indoleamine metabolism was largely unaffected at this time. Bilateral lesions of the nucleus basalis magnocellularis increased somatostatin and neuropeptide Y in the cortex of the rat by at least 138 and 284 percent, respectively, suggesting a functional interaction between cholinergic and peptidergic neurons that may differ from that in Alzheimer's disease.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Arendash, G W -- Millard, W J -- Dunn, A J -- Meyer, E M -- HD 17933/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1987 Nov 13;238(4829):952-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, University of South Florida, Tampa 33620.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2890210" target="_blank"〉PubMed〈/a〉
    Keywords: Acetylcholinesterase/metabolism ; Animals ; Biogenic Amines/metabolism ; Brain/metabolism/*pathology ; Cerebral Cortex/metabolism/*pathology ; Choline/metabolism ; Choline O-Acetyltransferase/metabolism ; Male ; Neuropeptide Y/analysis ; Olivary Nucleus/*physiology ; Organ Specificity ; Rats ; Rats, Inbred Strains ; Somatostatin/analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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