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  • FLUID MECHANICS AND HEAT TRANSFER  (8)
  • GEOPHYSICS  (6)
  • Life and Medical Sciences  (4)
  • Astronomy
  • ddc:330
  • 1985-1989  (18)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Adhesion defective BHK cell mutant has cell surface heparan sulfate proteoglycan of altered properties (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 136 (1988), S. 226-236 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In the light of accumulating data that implicate cell surface heparan sulfate proteoglycans (HSPGs) with a role in cell interactions with extracellular matrix molecules such as fibronectin, we have compared the properties of these molecules in wild-type BHK cells and an adhesion-defective ricin-resistant mutant (RicR14). Our results showed that the mutant, unlike BHK cells, cannot form focal adhesions when adherent to planar substrates in the presence of serum. Furthermore, while both cell lines possess similar amounts of cell surface HSPG with hydrophobic properties, that of RicR14 cells had decreased sulfation, reduced affinity for fibronectin and decreased half-life on the cell surface when compared to the normal counterpart. Our conclusions based on this data are that these altered properties may, in part, account for the adhesion defect in the ricin-resistant mutant. Whether this results from the known alteration in assembly of N-linked glycans affecting the carbohydrate chains on the proteoglycan or some other combination of factors is discussed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041360204
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Functional differences in the interactions of glycosylation-deficient cell lines with fibronectin, laminin, and type IV collagen (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 128 (1986), S. 402-412 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Fibronectin isolated from the conditioned medium of monolayer cultures of baby hamster kindey (BHK) cells and several ricin-resistant (Ric®) mutants derived from them express differences in N-glycosylation. The asparaginelinked oligosaccharides of BHK cell-derived fibronectin consist largely of complex chains, whereas hybrid and/or high-mannose chains are present in the fibronectins of mutant cell lines. The fibronectins exhibiting different glycosylation patterns are incorporated to similar extents into the cell-layer of human skin fibroblasts. In contrast, mutant cells retain significantly less endogenously produced fibronectin than BHK cells and also incorporate less human cellular fibronectin into a pericellular matrix. In vitro adhesion assays show that mutant cells attach to and spread relatively poorly on fibronectinor type IV collagen-coated substrata but interact as well as do BHK cells with a laminin substratum. These results indicate that asparagine-linked oligosaccharides of fibronectin are not required for the binding and incorporation of the molecule into cell layers, but, as constituents of other cellular glycoproteins, they do modulate the ability of BHK cells to interact with some matrix components.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041280309
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetics of calcium-binding proteins in yeasts (1987)
    New York, NY : Wiley-Blackwell
    BioEssays 6 (1987), S. 229-231 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950060509
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  • 4
    Electronic Resource
    Electronic Resource
    Nuclear plasmids in the Dictyostelium slime molds (1988)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 9 (1988), S. 495-504 
    Details
    ISSN: 0192-253X
    Keywords: transformation ; extrachromosomal DNAs ; eukaryotic plasmids ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Cellular slime molds are one of only three types of eukaryotes known to contain circular nuclear plasmids. Unlike the 2-μm circle in Saccharomyces, different strains of Dictyostelium can carry different, nonhomologous plasmids. Covalently closed, circular DNA plasmids have been identified in D. discoideum, D. mucoroides, D. giganteum, and D. purpureum. These plasmids range in size from 1.3-27 kb and in copy number from 50-300 molecules per cell. Plasmids have been identified in approximately one-fifth of all isolates examined. The organization of their DNA in nucleosomes establishes their presence in the nucleus. We have successfully cotransformed endogenous Dictyostelium plasmids into D. discoideum using the G418 resistance shuttle vector B10S. Transformants carrying D. discoideum plasmids are recovered at much higher frequency than those carrying plasmids from the other Dictyostelium species. We have constructed recombinant plasmids based on the D. discoideum plasmid Ddp2 and the G418 resistance gene. With these extrachromosomal vectors, transformed cells are recovered at frequencies of up to 10-4 per input cell, the vectors are stably maintained at high copy number in the absence of selection, and the vectors can be used to introduce foreign DNA sequences into D. discoideum cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020090426
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  • 5
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    Enhancements of energetic ions associated with travelling compression regions in the deep geomagnetic tail (1987)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: This paper presents a representative example of an enhancement in energetic ion flux associated with the International Sun-Earth Explorer 3 (ISEE 3) spacecraft's encounter with a traveling compression region (TCR). Data from the energetic particle anisotropy spectrometer (EPAS) instrument on ISEE 3 are studied, along with magnetic field data from the vector helium magnetometer. It is concluded that the ion enhancements seen are spatial in nature, thus supporting the idea that TCRs are the lobe signatures of plasmoids moving along the magnetotail, away from earth.
    Keywords: GEOPHYSICS
    Type: Journal of Geophysical Research (ISSN 0148-0227); 92; 64-70
    Format: text
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  • 6
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    CDAW 8 observations of plasmoid signatures in the geomagnetic tail - An assessment (1989)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: Magnetotail observations from the ISEE 3 distant (1983) tail mission taken during the Coordinated Data Analysis Workshop 8 (CDAW 8) A and G events are investigated. The ISEE 3 magnetic field, plasma, and energetic particle measurements taken in these two plasmoids have been analyzed and compared with various equilibrium structures and propagating waves/tail oscillation modes. Results indicate general agreement with either the closed-loop (Hones, 1977) or very small pitch angle flux rope (Hughes and Sibeck, 1987; Birn et al., 1989) models of plasmoid structure and poorer agreement with other hypotheses. Calculations based upon typical plasmoid and tail parameters are presented, indicating that the J and B force associated with the disconnected lobe field lines may be sufficient to accelerate plasmoids up to the speeds observed by ISEE 3. Overall, the energy expended in accelerating the plasmoids down the tail appears comparable to that dissipated in the inner magnetosphere and ionosphere. The study produces strong evidence in favor of the plasmoid model of substorm tail dynamics.
    Keywords: GEOPHYSICS
    Type: Journal of Geophysical Research (ISSN 0148-0227); 94; 15153-15
    Format: text
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  • 7
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    Dynamics of face and annular seals with two-phase flow (1989)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: A detailed study was made of face and annular seals under conditions where boiling, i.e., phase change of the leaking fluid, occurs within the seal. Many seals operate in this mode because of flashing due to pressure drop and/or heat input from frictional heating. High pressure, water pumps, industrial chemical pumps, and cryogenic pumps are mentioned as a few of many applications. The initial motivation was the LOX-GOX seals for the space shuttle main engine, but the study was expanded to include any face or annular seal where boiling occurs. Some of the distinctive behavior characteristics of two-phase seals were discussed, particularly their axial stability. While two-phase seals probably exhibit instability to disturbances of other degrees of freedom such as wobble, etc., under certain conditions, such analyses are too complex to be treated at present. Since an all liquid seal (with parallel faces) has a neutral axial stiffness curve, and is stabilized axially by convergent coning, other degrees of freedom stability analyses are necessary. However, the axial stability behavior of the two-phase seal is always a consideration no matter how well the seal is aligned and regardless of the speed. Hence, axial stability is thought of as the primary design consideration for two-phase seals and indeed the stability behavior under sub-cooling variations probably overshadows other concerns. The main thrust was the dynamic analysis of axial motion of two-phase face seals, principally the determination of axial stiffness, and the steady behavior of two-phase annular seals. The main conclusions are that seals with two-phase flow may be unstable if improperly balanced. Detailed theoretical analyses of low (laminar) and high (turbulent) leakage seals are presented along with computer codes, parametric studies, and in particular a simplified PC based code that allows for rapid performance prediction. A simplified combined computer code for the performance prediction over the laminar and turbulent ranges of a two-phase seal is described and documented. The analyses, results, and computer codes are summarized.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: NASA-CR-4256 , E-4999 , NAS 1.26:4256
    Format: application/pdf
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  • 8
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    Analysis of some fully-discrete algorithms for the one-dimensional heat equation (1985)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: The present investigation is concerned with a fully discrete accuracy and stability analysis of the one-dimensional heat equation, taking into account the evaluation of two-pass explicit schemes which simultaneously employ lumped and coupled capacity matrices. Schemes of the considered characteristics are not amenable to uncoupled semidiscrete and ordinary differential equation analyses. The obtained results illustrate that superior behavior may be achieved by schemes of the employed type when compared with the performance of the standard one-pass explicit schemes. The key idea in the considered approach is related to the utilization of a reduced-quadrature capacity matrix in the evaluation of the right-hand-side residual.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: International Journal for Numerical Methods in Engineering (ISSN 0029-5981); 21; 163-168
    Format: text
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  • 9
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    On the 3-dimensional structure of plasmoids (1987)
    In:  Other Sources
    Details
    Publication Date: 2019-07-12
    Description: The hypothesis that the IMF penetrates plasmoids causing them to be three- rather than two-dimensional is tested by comparing observations of By within plasmoids and related tail structures to upstream IMF By data. The magnetic topologies that result from the mergings of closed plasma sheet flux tubes and open tail lobe flux tubes at a near-earth neutral line, and merging near the tail flanks are described and studied. The particle signals and isotropic electron distributions are examined. It is observed that the IMF By penetrates plasmoids and that their structure is three-dimensional. In the three-dimensional model of plasmoids the reconnected plasma sheet field lines form a magnetic flux-ropelike structure. The three-dimensional model is utilized to analyze stagnant, slowly moving and earthward moving structures.
    Keywords: GEOPHYSICS
    Type: Geophysical Research Letters (ISSN 0094-8276); 14; 636-639
    Format: text
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  • 10
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    Recent progress in the development and understanding of SUPG methods with special reference to the compressible Euler and Navier-Stokes equations (1987)
    In:  Other Sources
    Details
    Publication Date: 2019-07-12
    Description: The current status of streamline-upwind/Petrov-Galerkin (SUPG) methods for the analysis of flow problems is surveyed in an analytical review. Problem areas addressed include classical Galerkin, upwind, artificial-diffusion, SUPG, discontinuous Galerkin, space-time FEM, and discontinuity-capturing approaches to the scalar advection-diffusion equation; incompressible flows; advective-diffusive systems; and the compressible Euler and Navier-Stokes equations. Graphs and diagrams are provided, and the good stability properties of state-of-the-art SUPG methods are pointed out.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: International Journal for Numerical Methods in Fluids (ISSN 0271-2091); 7; 1261-127
    Format: text
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