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  • FLUID MECHANICS AND HEAT TRANSFER  (8)
  • GEOPHYSICS  (6)
  • Engineering  (4)
  • Gossypium hirsutum  (3)
  • Astronomy
  • ddc:330
  • 1985-1989  (21)
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  • 1
    ISSN: 1617-4623
    Keywords: Restriction fragment length polymorphisms ; Gossypium hirsutum ; Gene copy number ; Lea, vicilin, and legumin genes ; Tetraploidization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have determined the copy number of 21 genes in an allotetraploid and several diploid species of cotton by gel and dot blot hybridization with cloned cDNAs. The legumin A, legumin B, and all 18 unique Lea (late embryogenesis-abundant) cDNA sequences isolated from the AD allotetraploid Gossypium hirsutum are present in one copy in A, D, E, and F diploid species and in two copies in G. hirsutum. Gel blot analysis of DNAs digested with EcoRI or BamHI usually detects different sized fragments in A and D diploids. Conservation of these restriction fragment length polymorphisms in G. hirsutum allows most of these fragments to be assigned to their respective subgenomes. Furthermore, both subgenomes in G. hirsutum can be distinguished from those in the interfertile allotetraploid G. barbadense. These results show that physical mapping of both sets of chromosomes in an allotetraploid should be possible by segregation analysis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 9 (1987), S. 301-313 
    ISSN: 1573-5028
    Keywords: Gossypium hirsutum ; embryo culture ; two-dimensional gel electrophoresis ; alloallele expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In earlier studies, only two major patterns of transcript accumulation were seen for 18 late embryogenesis abundant (Lea) gene families in cotton (Gossypium hirsutum L.) during embryogenesis and early germination. Each of these gene families probably comprises two active alloalleles. The two polypeptides encoded by seven of the Lea families can be distinguished, and analysis of their translation in vitro indicated that regulation of the homeologous transcript abundance was similar in each. In the present study, two-dimensional gel electrophoresis of polypeptides synthesized in excised embryos was employed to determine if LEA polypeptide synthesis is regulated at the translational level. The relative in vivo synthesis rate of each of the two polypeptides of 7 Lea families was compared with the relative concentration of their transcripts measured earlier by in vitro translation. For 4 families, the relative translational efficiencies of the homeologous mRNAs do not change during embryogenesis. However, there are changes of 1.5–3-fold in the other 3 families. The translation efficiencies of all transcripts of 9 of the Lea family mRNAs in vivo can be calculated from the fraction of total protein radioactivity incorporated in each LEA polypeptide family and the fractional abundance of Lea family transcripts measured by RNA dot hybridization. Lea mRNAs are found to be translated at near average rates throughout embryogenesis and early germination. These observations suggest that regulation of Lea gene expression at the translational level is minor.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 7 (1986), S. 155-170 
    ISSN: 1573-5028
    Keywords: embryo maturation ; cDNA cloning ; Gossypium hirsutum ; hybrid-arrest translation ; abscisic acid ; embryo culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Earlier studies found that cotton (Gossypium hirsutum L.) cotyledons contain several mRNAs which are more abundant during late embryogenesis than in mid-embryogenesis or early germination. They are here termed ‘Late embryogenesis-abundant’ mRNAs, encoded by Lea loci. Complementary DNA clones for 18 such mRNA sequences, defined at a hybridization criterion of Tm-15°C, were identified in a mature embryo cDNA library by differential cDNA hybridization. At a lower hybridization criterion, some sequence homology was found within several of these cloned Lea mRNA sequences. Each Lea mRNA sequence comprises 0.04–1.3% of mature embryo poly(A)+ mRNA, a level ten-fold to several hundred-fold higher than in young embryo or 24 h seedling poly(A)+ mRNA. Of 18 Lea mRNA sequences examined in cultured young embryos, the level of at least 13 are specifically increased by exogenous abscisic acid (ABA), several to a level near that in normal mature embryos. However, the abundance of several of the sequences does not appear to be significantly modulated by ABA. The LEA polypeptides encoded by 10 Lea mRNA sequences were identified by hybrid-arrested translation. They include most of the late embryogenesis-abundant, ABA-inducible, polypeptides previously identified. Preliminary results suggest that many of the individual Lea mRNA sequences are transcribed from 1–3 genes in each of cotton's two subgenomes.
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  • 4
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 21 (1985), S. 163-168 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A fully discrete stability and accuracy analysis of some algorithms for the one-dimensional heat equation is presented. Results illustrate that 2-pass explicit schemes which simultaneously employ lumped and coupled capacity matrices are capable of improved performance over the standard 1-pass explicit scheme.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 28 (1989), S. 1485-1485 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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  • 6
    ISSN: 0271-2091
    Keywords: Advection-diffusion equation ; Advective-diffusive systems ; Artificial-diffusion ; Compressible flows ; Discontinuous Galerkin method ; Entropy Error analysis ; Euler equations ; Finite elements ; Galerkin method ; Hyperbolic systems ; Incompressible flow ; Navier-Stokes equations ; Petrov-Galerkin method ; Space-time formulation ; Upwind methods ; Weighted residual methods ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: SUPG methods were originally developed for the scalar advection-diffusion equation and the incompressible Navier-Stokes equations. In the last few years successful extensions have been made to symmetric advective-diffusive systems and, in particular, the compressible Euler and Navier-Stokes equations. New procedures have been introduced to improve resolution of discontinuities and thin layers. In this paper a brief overview is presented of recent progress in the development and understanding of SUPG methods.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0748-8025
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: If bending moments converge at a sufficiently fast rate, it is proved that transverse shear stress resultants calculated from the moment equilibrium equation also converge. The result holds independent of the plate theory or type of element employed.
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  • 8
    Publication Date: 2011-08-19
    Description: This paper presents a representative example of an enhancement in energetic ion flux associated with the International Sun-Earth Explorer 3 (ISEE 3) spacecraft's encounter with a traveling compression region (TCR). Data from the energetic particle anisotropy spectrometer (EPAS) instrument on ISEE 3 are studied, along with magnetic field data from the vector helium magnetometer. It is concluded that the ion enhancements seen are spatial in nature, thus supporting the idea that TCRs are the lobe signatures of plasmoids moving along the magnetotail, away from earth.
    Keywords: GEOPHYSICS
    Type: Journal of Geophysical Research (ISSN 0148-0227); 92; 64-70
    Format: text
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  • 9
    Publication Date: 2011-08-19
    Description: Magnetotail observations from the ISEE 3 distant (1983) tail mission taken during the Coordinated Data Analysis Workshop 8 (CDAW 8) A and G events are investigated. The ISEE 3 magnetic field, plasma, and energetic particle measurements taken in these two plasmoids have been analyzed and compared with various equilibrium structures and propagating waves/tail oscillation modes. Results indicate general agreement with either the closed-loop (Hones, 1977) or very small pitch angle flux rope (Hughes and Sibeck, 1987; Birn et al., 1989) models of plasmoid structure and poorer agreement with other hypotheses. Calculations based upon typical plasmoid and tail parameters are presented, indicating that the J and B force associated with the disconnected lobe field lines may be sufficient to accelerate plasmoids up to the speeds observed by ISEE 3. Overall, the energy expended in accelerating the plasmoids down the tail appears comparable to that dissipated in the inner magnetosphere and ionosphere. The study produces strong evidence in favor of the plasmoid model of substorm tail dynamics.
    Keywords: GEOPHYSICS
    Type: Journal of Geophysical Research (ISSN 0148-0227); 94; 15153-15
    Format: text
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  • 10
    Publication Date: 2019-06-28
    Description: A detailed study was made of face and annular seals under conditions where boiling, i.e., phase change of the leaking fluid, occurs within the seal. Many seals operate in this mode because of flashing due to pressure drop and/or heat input from frictional heating. High pressure, water pumps, industrial chemical pumps, and cryogenic pumps are mentioned as a few of many applications. The initial motivation was the LOX-GOX seals for the space shuttle main engine, but the study was expanded to include any face or annular seal where boiling occurs. Some of the distinctive behavior characteristics of two-phase seals were discussed, particularly their axial stability. While two-phase seals probably exhibit instability to disturbances of other degrees of freedom such as wobble, etc., under certain conditions, such analyses are too complex to be treated at present. Since an all liquid seal (with parallel faces) has a neutral axial stiffness curve, and is stabilized axially by convergent coning, other degrees of freedom stability analyses are necessary. However, the axial stability behavior of the two-phase seal is always a consideration no matter how well the seal is aligned and regardless of the speed. Hence, axial stability is thought of as the primary design consideration for two-phase seals and indeed the stability behavior under sub-cooling variations probably overshadows other concerns. The main thrust was the dynamic analysis of axial motion of two-phase face seals, principally the determination of axial stiffness, and the steady behavior of two-phase annular seals. The main conclusions are that seals with two-phase flow may be unstable if improperly balanced. Detailed theoretical analyses of low (laminar) and high (turbulent) leakage seals are presented along with computer codes, parametric studies, and in particular a simplified PC based code that allows for rapid performance prediction. A simplified combined computer code for the performance prediction over the laminar and turbulent ranges of a two-phase seal is described and documented. The analyses, results, and computer codes are summarized.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: NASA-CR-4256 , E-4999 , NAS 1.26:4256
    Format: application/pdf
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