Publication Date:
1985-09-06
Description:
Melanocytes derived from fetal or adult skin do not propagate in vitro unless cultured in the presence of factors such as 12-O-tetradecanoylphorbol 13-acetate (TPA). In a search for physiological factors regulating the growth of melanocytes, extracts of various cultured cell types were tested. Factors produced by melanoma and astrocytoma cell lines support continued proliferation of melanocytes in the absence of TPA. WI-38, a fibroblast cell line derived from human embryonic lung, was the most active source of melanocyte growth factors. No melanocyte growth-promoting activity was found in extracts of cultured neuroblastoma, renal cancer, normal keratinocytes, or renal epithelium. Nerve growth factor, epidermal growth factor, melanocyte-stimulating hormone, transforming growth factor-beta, and platelet-derived growth factor did not have growth-promoting activity for melanocytes. The presence of melanocyte growth factors and TPA together resulted in the strongest mitogenic activity for melanocytes, permitting the recovery (at 20 days) of 4 to 20 times as many cells as in growth factor or TPA alone.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Eisinger, M -- Marko, O -- Ogata, S -- Old, L J -- 1R01 CA-32152/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Sep 6;229(4717):984-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/4023718" target="_blank"〉PubMed〈/a〉
Keywords:
Astrocytoma/physiopathology
;
Cell Division/drug effects
;
Cell Line
;
Cholera Toxin/pharmacology
;
Fibroblasts/physiology
;
Growth Substances/isolation & purification/*pharmacology
;
Humans
;
Melanocytes/*cytology
;
Melanoma/physiopathology
;
Tetradecanoylphorbol Acetate/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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