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  • Life and Medical Sciences  (948)
  • AERODYNAMICS  (829)
  • EARTH RESOURCES AND REMOTE SENSING  (544)
  • Lunar and Planetary Science and Exploration
  • 1985-1989  (2,329)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 8 (1987), S. 312-323 
    ISSN: 0886-1544
    Keywords: plant cytoskeleton ; Chlamydomonas ; anti-IFA ; onion root tip cells ; immunoflurescence ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Four monoclonal antibodies were raised against polypetides present in a highsalt detergent-insoluble fraction from cells of Chlamydomonas reinhardtii. Indirect immunofluorescence microscopy of fibroblasts and epithelial cells grown in culture using these plant antibodies revealed staining arrays identical to those obtained with well characterised antibodies to animal intermediate filaments. Immunoflurescence microscopy of Chlamydomonas with these monoclonal antibodies and a monoclonal antibody that recognises all animal intermediate filaments (anti-IFA) gave a diffuse, patchy cytoplasmic staining pattern. Both the plant antibodies and anti-IFA stained interphase onion root tip cells in a diffuse perinuclear pattern. In metaphase through to telophase, the labelling patterns colocalised with those of microtubules. Labelling of the phragmoplast was also detected but not staining of the preprophase band. On Western blots of various animal cell lines and tissues, all the antibodies labelled known intermediate filament proteins. On Western blots of whole Chlamydomonas proteins, all the antiboides labelled a broad band in the 57,000 Mr range, and three antibodies labelled bands around 66,000 and 140,000 Mr but with varibale intensites. On Western blots of whole onion root tip proteins, all the antibodies labelled 50,0000 Mr (two to three bands) polypetides and a diffuse and around 60,000 Mr and three of the antibodies also labelled several polypeptides in the 90,000-200,000 Mr range. The consistent labelling of these different bands by several different monoclonal antibodies recognising animal intermediate filaments makes these polypetides putative plant intermediate filament proteins.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 24 (1989), S. 205-218 
    ISSN: 0148-7280
    Keywords: oocyte aging ; immature oocytes ; oocyte maturation ; chromosome abnormalities ; in vitro fertilization ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: It has been suggested that the abnormal maturation of the human oocyte during fertilization in vitro may result in chromosome imbalance and, induce embryonic loss. Using a mouse model, we have studied the influence of the degree of oocyte maturation (either immaturity or overmaturity) on the chromosome characteristics of embryos at the first-cleavage division. Immature oocytes were obtained 2-3 h or 3-4 h before the expected ovulation time (b.o.). Overmaturation was induced by aging the newly ovulated oocytes in vitro for 3,6, and 12 h. Our results show a significant decrease in the fertilization rate in the immature groups (65.53% at 2-3 h b.o. and 16.59% at 3-4 h b.o. vs. 78.22% at control) and after 12 h of in vitro aging (69.39%), while a significant increase of this parameter was found at 3 h of aging (82.59%) as compared to the other groups. No significant differences were found in the occurrence of aneup'.oidy or hypcrhaploidy in embryos obtained from immature, newly ovulated, and overmature oocytes. Finally, an increased incidence of polyploidy was detected in immature, 2-3 h b.o. (31.20%), and overmature, 3 h (23.04%) and 6 h (31.61%), groups as compared to the control group (14.59%).
    Additional Material: 3 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 21 (1988), S. 93-111 
    ISSN: 0148-7280
    Keywords: sperm-oocyte fusion ; reactive oxygen species ; motility ; calcium ; cAMP ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The possible role of calmodulin in regulating a number of calcium-dependent functions exhibited by human spermatozoa was investigated by using the antagonists trifluoperazine and calmidazolium. At high doses both antagonists inhibited the motility of human spermatozoa and induced a concomitant rise in [Ca2+]i and a decline in cAMP. Lower doses of these antagonists, particularly calmidazolium, suppressed the ability of human spermatozoa to generate reactive oxygen species and exhibit sperm-oocyte fusion, without influencing [Ca2+]i, cAMP, or motility. This inhibition of sperm-oocyte fusion was effective even if the spermatozoa were subsequently exposed to A23187, suggesting that calmodulin may regulate this aspect of human sperm function at a point downstream from calcium influx.Both radiolabelling and affinity chromatography techniques were used to detect a number of calcium-dependent and calcium-independent calmodulin acceptor proteins in the human spermatozoon. The major calcium-dependent acceptor proteins exhibited Mr values of 32,000 and 22,000-27,000, respectively, and did not appear to be associated with the sperm plasma membrane.
    Additional Material: 9 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 21 (1988), S. 185-192 
    ISSN: 0148-7280
    Keywords: oocyte ; fusion ; protein synthesis inhibition ; maturation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The method of polyethylene-glycol-induced fusion of mammalian oocytes was applied to study maturation-promoting factor (MPF) activity. After homologous fusions of one maturing-late diakinesis (LD), metaphase I (MI)-pig or mouse oocyte to one, two, or three immature-germinal vesicle (GV)-oocytes, giant cells were cultured in control or cycloheximide supplemented medium for 3 hours. The occurrence of germinal vesicle breakdown (GVBD) and premature chromosome condensation (PCC) served as a control of MPF activity. In giant cells composed of one maturing and one, two, or three immature oocytes, GVBD and PCC were observed in all cases after cultivation in the control medium. In the presence of cycloheximide, the completion of GVBD and PCC remained high when one maturing and one immature oocyte were fused (83.7% and 95.7% of GVBD in pig and mouse, respectively). However, in giant cells composed of one maturing and up to three immature oocytes, all GVs were broken down only occasionally (4.8% and 11.7% in pig and mouse, respectively). These results suggest that in pig and mouse oocytes MPF does not amplify autocatalytically, but requires active protein synthesis for its production.
    Additional Material: 3 Ill.
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  • 5
    Publication Date: 2011-08-19
    Description: A program is presented to perform coordinated global experiments designed to use the unique features of synthetic aperture radar (SAR) sensors such as the ones on ERS-1, SIR-C and EOS to characterize the physical nature of forest stands as input to global ecosystem and climatology models. Details about the objectives, program and expected results are presented.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
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  • 6
    Publication Date: 2014-09-17
    Description: The principal objectives of the UK SATMaP program are to determine thematic mapper (TM) performance with particular reference to spatial resolution properties and geometric characteristics of the data. So far, analysis is restricted to images from the U.S. and concentrates on spectra and radiometric properties. The results indicate that the data are inherently three dimensional compared with the two dimensional character of MSS data. Preliminary classification results indicate the importance of the near infrared band (TM 4), at least one middle infrared band (TM 5 or TM 6) and at least one of the visible bands (preferably either TM 3 or TM 1). The thermal infrared also appears to have discriminatory ability despite its coarser spatial resolution. For band 4 the forward and reverse scans show somewhat different spectral responses in one scene but this effect is absent in the other analyzed. From examination of the histograms it would appear that the full 8 bit quantization is not being effectively utilized for all the bands.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: NASA. Goddard Space Flight Center LANDSAT-4 Sci. Characterization Early Results, Vol. 4; p 369-386
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  • 7
    Publication Date: 2011-08-24
    Description: The infrared instrument IKS flown on board the VEGA space probes was designed for the detection of emission bands of parent molecules, and for a measurement of the size and temperature of the thermal emitting nuclear region. The instrument had three channels with cooled detectors: an "imaging channel" designed to modulate the signal of the nucleus and two spectroscopic channels operating at 2.5-5 and 6-12 micrometers, respectively, equipped with circular variable filters of resolving power approximately 50. This paper presents and discusses the results from the spectral channels. On VEGA 1, usable spectra were obtained at distances D from the comet nucleus ranging from 250,000 to 40,000 km corresponding to fields of view 4000 and 700 km in diameter, respectively. The important internal background signal caused by the instrument itself, which could not be cooled, had to be eliminated. Since no sky chopping was performed, we obtain difference spectra between the current spectrum and a reference spectrum with little or no cometary signal taken at the beginning of the observing sequence (D approximately 200,000 km). Final discrimination between cometary signal and instrumental background is achieved using their different time evolution, since the instrumental background is proportional to the slow temperature drift of the instrument, and the cometary signal due to parent molecules or dust grains is expected to vary in first order as D-1. The 2.5-5 micrometers IKS spectra definitely show strong narrow signals at 2.7 and 4.25 micrometers, attributed to the nu 3 vibrational bands of H2O and CO2, respectively, and a broader signal in the region 3.2-3.5 micrometers, which may be attributed to CH-bearing molecules. All these signals present the expected D-1 intensity variation. Weaker emission features at 3.6 and 4.7 micrometers could correspond to the nu 1 and nu 5 bands of H2CO and the (1 - 0) band of CO, respectively. Molecular production rates are derived from the observed emissions, assuming that they are due to resonance fluorescence excited by the Sun's infrared radiation. For the strong bands of H2O and CO2, the rovibrational lines are optically thick, and radiative transfer is taken into account. We derive production rates, at the moment of the VEGA 1 flyby, of approximately 10(30) sec-1 for H2O, approximately 2.7 x 10(28) sec-1 for CO2, approximately 5 x 10(28) sec-1 for CO, and 4 x 10(28) sec-1 for H2CO, if attributions to CO and H2CO are correct. The production rate of carbon atoms in CH-bearing molecules is approximately 9 x 10(29) sec-1 assuming fluorescence of molecules in the gas phase, but could be much less if the 3.2-3.5 micrometers emission is attributed to C-H stretch in polycyclic aromatic hydrocarbons or small organic grains. In addition, marginal features are present at 4.85 and 4.45 micrometers, tentatively attributed to OCS and molecules with the CN group, respectively. Broad absorption at 2.8-3.0 micrometers, as well as a narrow emission at 3.15 micrometers, which follow well the D-1 intensity variation, might be due to water ice. Emission at 2.8 micrometers is also possibly present, and might be due to OH created in vibrationally excited states after water photodissociation. The 6-12 micrometers spectrum does not show any molecular emission, nor emission in the 7.5-micrometers region. The spectrum is dominated by silicate emission showing a double structure with maxima at 9.0 and 11.2 micrometers, which suggests the presence of olivine.
    Keywords: Lunar and Planetary Science and Exploration
    Type: Icarus (ISSN 0019-1035); Volume 76; 404-36
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  • 8
    Publication Date: 2019-06-28
    Description: Two complementary sensors, the DAEDALUS DEI-1260 Multispectral Scanner aboard the NASA U-2 aircraft and the Advanced Very High Resolution Radiometer aboard National Oceanographic and Atmospheric Administration orbiting satellites were tested for their applicability in monitoring and predicting parameters such as fire location, temperature and rate of spread, soil heating and cooling rates, and plume characteristics and dimensions. In addition, the satellite system was tested for its ability to extend the relationships found between fire characteristics and biospheric consequences to regional and global scales. An overall system design is presented, and special requirements are documented for the application of this system for fire research and management.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: AIAA PAPER 87-0187
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  • 9
    Publication Date: 2019-07-12
    Description: This paper develops and analyses individual construction aspects of an efficient and accurate finite element algorithm for prediction of viscous and turbulent flow fields of impact in aerodynamics. The theoretical construction employs a Taylor weak statement (TWS) for coincident embedding of stability mechanisms within a classic Galerkin finite element formulation of semidiscrete approximation error orthogonalization. A wide variety of the stabilizing mechanisms of independently derived CFD algorithms are contained within the TWS theory. An implicit construction that meets the requirement of efficient convergence to steady state is developed. The theoretical asymptotic error estimates of the TWS finite element algorithm for supersonic and viscous boundary layer flows are verified. Application to a three-dimensional turbulent flow is cited.
    Keywords: AERODYNAMICS
    Type: International Journal for Numerical Methods in Fluids (ISSN 0271-2091); 7; 1235-125
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  • 10
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This investigation relates the occlusal morphology of the continuously growing molars of common wombats (Vombatus ursinus) to the underlying enamel ultrastructure that was investigated using the techniques of light microscopy, scanning electron microscopy, and transmission electron microscopy. The main feature of the occlusal enamel was a prominent ridge, which followed the contour of the dentine-enamel junction (DEJ). It was found that the occlusal morphology depended upon the orientation of the dentinal and enamel tissues, variations in prism orientation, Hunter-Schreger bands (HSB), and presence or absence of cleavage. Cleavage of enamel promoted by sheets of parallel prisms occurred along the face between the DEJ and the ridge, whereas on the face between the ridge and the cementum-enamel junction (CEJ) cleavage was inhibited by HSB. The slope of the latter face was mainly due to a decrease in wear resistance going from the ridge, where prisms were intercepted transversely, toward the CEJ, where they were intercepted obliquely. Occasionally small surface undulations were observed on the face between the ridge and the CEJ. These undulations were found to correspond to gradually decussating enamel regions. The pronounced cleavage of enamel parallel to the face between the DEJ and the ridge played an important role in conferring on the continuously growing molars a distinct property to develop and maintain a self-sharpening ridge throughout the life of the tooth.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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