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  • Cell & Developmental Biology  (568)
  • AERODYNAMICS  (555)
  • METEOROLOGY AND CLIMATOLOGY  (279)
  • 1985-1989  (1,402)
  • 1
    Electronic Resource
    Electronic Resource
    Differential expression of metastasis-associated cell surface glycoproteins and mRNA in a murine large cell lymphoma (1986)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 31 (1986), S. 305-312 
    Details
    ISSN: 0730-2312
    Keywords: tumor metastasis ; gene expression ; oncogenes ; virus antigens ; glycoproteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A metastatic variant cell subline of the Abelson virus-transformed murine large lymphoma/lymphosarcoma RAW 117 has been selected in vivo ten times for liver colonization. Highly metastatic subline RAW117-H10 forms greater than 200 times as many gross surface liver tumor nodules as the parental line RAW117-P. Analysis of cellular proteins and glycoproteins indicates reduced expression of murine Moloney leukemia virus-associated p15, p30, and gp70, and increased expression of a sialoglycoprotein, gp150, in the highly metastatic H10 cells. Northern analyses of oncogene expression suggested that mRNA of various oncogenes was expressed equally or not expressed in the RAW117 cells of differing metastatic potential. Differential gene expression was examined using a cDNA library of 17,600 clones established from poly A+ mRNA isolated from H10 cells. The cDNA library was screened by the colony hybridization technique using probes made from both RAW117-P and -H10 cells. Approximately 99.5% of these cDNA clones were expressed identically in P and H10 cells. Of the few differentially expressed cDNA clones (approx. 150/17,600), one-half of these were identified as Moloney leukemia virus sequences in a separate probing with a radiolabeled Moloney leukemia virus probe. The remainder of the differentially expressed mRNA detected by colony hybridization of the cDNA library were expressed at higher levels (approx. 1/6) or lower levels (approx. 1/3) in the highly metastatic H10 cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240310408
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of sulfated glycoconjugates on capacitation and the acrosome reaction of bovine and hamster spermatozoa (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 24 (1989), S. 403-413 
    Details
    ISSN: 0148-7280
    Keywords: heparin ; fertilization ; dextran sulfate ; fucose sulfate glycoconjugate ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of sulfated glycoconjugates on the preparation of mammalian sperm for fertilization were investigated. The three sulfated glycoconjugates tested were heparin, dextran sulfate, and the fucose sulfate glycoconjugate (FSG) from the sea urchin egg jelly coat. In vivo, FSG induces the acrosome reaction in sea urchin sperm. Bovine sperm were found to be capacitated by heparin and FSG as judged both by ability of lysophosphatidylcholine (LC) to induce an acrosome reaction and by ability to fertilize bovine oocytes in vitro. The mechanism by which heparin or FSG capacitated bovine sperm appeared similar, since glucose inhibited capacitation by both glycoconjugates. In contrast to effects on bovine sperm, heparin and FSG induced the acrosome reaction in capacitated hamster sperm. When hamster sperm were incubated under noncapacitating conditions, heparin had no effect on capacitation or the acrosome reaction. Three molecular weights (MW) of dextran sulfate (5,000, 8,000, 500,000) were found to capacitate bovine sperm as judged by the ability of LC to induce an acrosome reaction. Whereas bovine sperm incubated with 5,000 or 8,000 M W dextran sulfate fertilized more bovine oocytes than control sperm (P 〈0.05), sperm treated with 500,000 M W dextran sulfate failed to penetrate oocytes. The high-MW dextran sulfate appeared to interact with the zona pellucida and/or sperm to prevent sperm binding. Results suggest that sulfated glycoconjugates may prepare sperm for fertilization across a wide range of species.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120240407
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  • 3
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    Nimbus-7 global cloud climatology. I - Algorithms and validation (1988)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: An improved version of the Nimbus-7 cloud retrieval algorithm was validated using data from Nimbus-7 Temperature Humidity Infrared Radiometer and Total Ozone Mapping Spectrometer to determine cloudiness parameters for the globe. Quantitative validation of total cloud amount was performed by comparing the algorithm results with estimates derived from GOES images and auxiliary meteorological data. The systematic errors of the Nimbus-7 total cloud-amount algorithm, relative to the GOES-derived estimates, were found to be less than 10 percent. The random errors of daily estimates ranged between 7 and 16 percent, day or night.
    Keywords: METEOROLOGY AND CLIMATOLOGY
    Type: Journal of Climate (ISSN 0894-8755); 1; 445-470
    Format: text
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    NASA TECHNICAL REPORTS
  • 4
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    Angular radiation models for Earth-atmosphere system. Volume 1: Shortwave radiation (1988)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: Presented are shortwave angular radiation models which are required for analysis of satellite measurements of Earth radiation, such as those fro the Earth Radiation Budget Experiment (ERBE). The models consist of both bidirectional and directional parameters. The bidirectional parameters are anisotropic function, standard deviation of mean radiance, and shortwave-longwave radiance correlation coefficient. The directional parameters are mean albedo as a function of Sun zenith angle and mean albedo normalized to overhead Sun. Derivation of these models from the Nimbus 7 ERB (Earth Radiation Budget) and Geostationary Operational Environmental Satellite (GOES) data sets is described. Tabulated values and computer-generated plots are included for the bidirectional and directional modes.
    Keywords: METEOROLOGY AND CLIMATOLOGY
    Type: NASA-RP-1184 , L-16414 , NAS 1.61:1184
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 5
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    Angular radiation models for earth-atmosphere system. Volume 2: Longwave radiation (1989)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: The longwave angular radiation models that are required for analysis of satellite measurements of Earth radiation, such as those from the Earth Radiation Budget Experiment (ERBE) are presented. The models contain limb-darkening characteristics and mean fluxes. Limb-darkening characteristics are the longwave anisotropic factor and the standard deviation of the longwave radiance. Derivation of these models from the Nimbus 7 ERB (Earth Radiation Budget) data set is described. Tabulated values and computer-generated plots are included for the limb-darkening and mean-flux models.
    Keywords: METEOROLOGY AND CLIMATOLOGY
    Type: NASA-RP-1184-VOL-2 , L-16503 , NAS 1.61:1184-VOL-2
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 6
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    Operating Characteristics of the Multiple Critical Venturi System and Secondary Calibration Nozzles Used for Weight-Flow Measurements in the Langley 16-Foot Transonic Tunnel (1985)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: An investigation has been conducted in the Langley 16 Foot Transonic Tunnel to determine the weight flow measurement characteristics of a multiple critical Venturi system and the nozzle discharge coefficient characteristics of a series of convergent calibration nozzles. The effects on model discharge coefficient of nozzle throat area, model choke plate open area, nozzle pressure ratio, jet total temperature, and number and combination of operating Venturis were investigated. Tests were conducted at static conditions (tunnel wind off) at nozzle pressure ratios from 1.3 to 7.0.
    Keywords: AERODYNAMICS
    Type: NASA-TM-86405 , L-15960 , NAS 1.15:86405
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 7
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    TRANAIR - A computer code for transonic analyses of arbitrary configurations (1987)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: Attention is given to a new approach to solving full potential equations about arbitrary configurations. Numerical algorithms from such fields as finite elements, preconditioned Krylov subspace methods, discrete Fourier analysis, and integral equations are combined to take advantage of the size and speed of current and emerging supercomputers. On the basis of this appraoch, a robust, efficient and easy to use computer code referred to as TRANAIR has been developed for transonic analysis of complex geometries.
    Keywords: AERODYNAMICS
    Type: AIAA PAPER 87-0034
    Format: text
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    NASA TECHNICAL REPORTS
  • 8
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    Application of an upwind Navier-Stokes code to two-dimensional transonic airfoil flow (1987)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: An upwind-biased implicit approximate factorization Navier-Stokes algorithm is applied to a variety of steady transonic airfoil cases, using the NACA 0012, RAE 2822, and Jones supercritical airfoils. The thin-layer form of the compressible Navier-Stokes equations is used. Both the CYBER 205 and CRAY 2 supercomputers are utilized, with average computational speeds of about 18 and 16 microsec/gridpoint/iteration, respectively. Lift curves, drag polars, and variations in drag coefficient with Mach number are determined for the NACA 0012 and Jones supercritical airfoils. Also, several cases are computed for comparison with experiment. The effect of grid density and grid extent on a typical turbulent airfoil solution is shown. An algebraic eddy-viscosity turbulence model is used for all of the computations.
    Keywords: AERODYNAMICS
    Type: AIAA PAPER 87-0413
    Format: text
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    NASA TECHNICAL REPORTS
  • 9
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    Finite-volume scheme for transonic potential flow about airfoils and bodies in an arbitrarily-shaped channel (1985)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: A conservative finite-volume difference scheme is developed for the potential equation to solve transonic flow about airfoils and bodies in an arbitrary channel. The scheme employs a mesh which is a nearly-conformal 'O' mesh about the airfoil and nearly orthogonal at the channel walls. The mesh extends to infinity upstream and downstream, where the mapping is singular. Special procedures are required to treat the singularities at infinity, including computation of the metrics near those points. Channels with exit areas different from inlet areas are solved; a body with a sting mount is an example of such a case.
    Keywords: AERODYNAMICS
    Type: Symposium on Numerical and Physical Aspects of Aerodynamic Flows; Jan 21, 1985 - Jan 24, 1985; Long Beach, CA
    Format: text
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    NASA TECHNICAL REPORTS
  • 10
    Electronic Resource
    Electronic Resource
    Spermiogenesis in the bullfrog (Rana catesbeiana): A study of cytoplasmic events including cell volume changes and cytoplasmic elimination (1988)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 198 (1988), S. 303-319 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The process by which spermatid cytoplasmic volume is reduced and cytoplasm eliminated during spermiogenesis was investigated in the bullfrog Rana catesbeiana. At early phases of spermiogenesis, newly formed, rounded spermatids were found within spermatocysts. As acrosomal development, nuclear elongation, and chromatin condensation occurred, spermatid nuclei became eccentric within the cell. A cytoplasmic lobe formed from the caudal spermatid head and flagellum and extended toward the seminiferous tubule lumen. The cytoplasmic lobe underwent progressive condensation whereby most of its cytoplasm became extremely electron dense and contrasted sharply with numerous electron-translucent vesicles contained therein. At the completion of spermiogenesis, many spermatids with their highly condensed cytoplasm still attached were released from their Sertoli cell into the lumen of the seminiferous tubule. There was no evidence of the phagocytosis of residual bodies by Sertoli cells. Because spermatozoa are normally retained in the testis in winter and are not released until the following breeding season, sperm were induced to traverse the duct system with a single injection of hCG. Some spermatids remained attached to their cytoplasm during the sojourn through the testicular and kidney ducts; however, by the time the sperm reached the Wolffian duct, separation had occurred. The discarded cytoplasmic lobe (residual body) appeared to be degraded within the epithelium of the Wolffian duct. It was determined that the volume of the spermatid was reduced by 87% during spermiogenesis through a nuclear volume decrease of 76% and cytoplasmic volume decrease of 95.3%.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051980305
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